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p35.pA.NGFR.mCMV.hPGK.mSrtA(Pasqual)TM.WPRE[sequenced]

Plasmid

#226760

Purpose

sortase A (Pasqual)- TM in p36

Depositor

Ferdinando Pucci

Article

Hamilton et al Adv Biosyst. 2020 Dec;4(12):e2000007. do

Insert

sortase A (srtA )

Expression

Mammalian

Available Since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAN102

Plasmid

#220045

Purpose

Mammalian expression of type III-A crRNA and Cas6 crRNA-processing ribonuclease from Streptococcus thermophilus

Depositor

Blake Wiedenheft

Article

Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

Insert

crRNA; Cas6

Expression

Mammalian

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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AA020

Plasmid

#216004

Purpose

Fragmid fragment: (Cas protein) binds PP7 stem loops

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

PP7 Core Protein (PCP)_v1.1

Use

CRISPR; Fragment

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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AA366

Plasmid

#215970

Purpose

Fragmid fragment: (Pol 2 promoter) Gal4-induced expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

5xUAS_v1; 5xUAS_v1; 5xUAS_v1; 5xUAS_v1; Hsp70_v2; Mhc_v1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA151

Plasmid

#215938

Purpose

Fragmid fragment: (guide cassette) for dual expression sgRNA

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; BsmBI_v0; BsmBI_v4; rev{H1_v1}

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA401

Plasmid

#215959

Purpose

Fragmid fragment: (guide cassette) guide expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; DR_v1 [Dj]; BsmBI_v1; BsmBI_v12

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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LI D-E TurboID-GFP

Plasmid

#209387

Purpose

Golden Gate LI D-E module

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Type

Empty backbone

Use

Cloning vector

Mutation

TurboID gene sequence C249A

Available Since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pJS237

Plasmid

#200713

Purpose

ADH2 terminator from yeast (MoClo 3´U +Ter)

Depositor

Jens Boch

Article

Fuhrmeister et al Bio Protoc. 2023 Aug 5;13(15):e4733. doi

Insert

ADH2 Terminator

Use

Synthetic Biology

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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NK809

Plasmid

#196406

Purpose

Strong expression of firefly and nanoluc luciferases codon-optimized for S. rosetta

Depositor

Nicole King

Article

Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

Insert

Sro5EFL-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin

Use

Luciferase

Mutation

N/A (synthetic)

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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NK810

Plasmid

#196407

Purpose

Nanoluc luciferase under S. rosetta SPAG6 promoter with internal actin-firefly control

Depositor

Nicole King

Article

Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

Insert

Sro5spag6-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin

Use

Luciferase

Mutation

N/A (synthetic)

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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NK811

Plasmid

#196408

Purpose

Nanoluc luciferase under S. rosetta SPAG6 promoter with mutated RFX binding site with internal actin-firefly control

Depositor

Nicole King

Article

Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

Insert

Sro5spag6_mut-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin

Use

Luciferase

Mutation

N/A (synthetic)

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pILAC2

Plasmid

#185889

Purpose

In vivo gene amplification (HapAmp) of lycopene synthetic module at RPL25 locus

Depositor

Claudia Vickers

Article

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Insert

see comments

Expression

Yeast

Mutation

ERG20 F96C; Xd.CRtYB E83K

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pFGC-T7RibJ-RRvT

Plasmid

#173226

Purpose

Encodes RRvT fluorescent protein. To be used in cell-free system

Depositor

Jim Haseloff

Article

Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

Insert

RRvT

Use

Cell-free system

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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SGP11_Halo_N_allele

Plasmid

#178171

Purpose

Donor vector for endogenous tagging of human SGP11 at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (SPG11 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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KCNC3_Halo_N_allele

Plasmid

#178129

Purpose

Donor vector for endogenous tagging of human KCNC3 at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (KCNC3 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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PLEKHG4_Halo_C_allele

Plasmid

#178128

Purpose

Donor vector for endogenous tagging of human PLEKHG4 at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (PLEKHG4 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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TIA1_Halo_N_allele

Plasmid

#178149

Purpose

Donor vector for endogenous tagging of human TIA1 at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (TIA1 Human)

Use

Donor vector

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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DAO_Halo_C_allele

Plasmid

#178155

Purpose

Donor vector for endogenous tagging of human DAO at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (DAO Human)

Use

Donor vector

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pZIC3-donor

Plasmid

#176361

Purpose

CRISPR donor plasmid to create GFP fusion proteins

Depositor

Barbara Stranger, Kevin White

Insert

ZIC3 (ZIC3 Human)

Use

CRISPR

Tags

GFP

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCHCHD3-donor

Plasmid

#176347

Purpose

CRISPR donor plasmid to create GFP fusion proteins

Depositor

Barbara Stranger, Kevin White

Insert

CHCHD3 (CHCHD3 Human)

Use

CRISPR

Tags

GFP

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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