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We narrowed to 4,443 results for: erf

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Showing: 4281 - 4300 of 4443 results

  8. pCMVtrunc sfCherry2ΔC4-Lifeact

    Plasmid
    #231557
    Purpose
    Mammalian expression of Lifeact fused to C-terminally truncated superfolder Cherry2, for actin filament organization measurements using fluorescence polarization microscopy
    Depositor
    Manos Mavrakis
    Article
    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.
    Insert
    Lifeact (ABP140 Budding Yeast)
    Tags
    sfCherry2
    Expression
    Mammalian
    Promoter
    CMVtrunc
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231557-plasmid-map-sequence-id-459687

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  9. Lenti-EFS-BirAopt-GSQ-RNF4ca-P2A-BLAST

    Plasmid
    #208047
    Purpose
    Enables constitutive expression of N-terminal BirAopt-fused RNF4ca (catalytically inactive RING mutant); to perform bioE3; selection with blasticidin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    RNF4 (RNF4 Human)
    Use
    Lentiviral
    Tags
    BirAopt
    Mutation
    Cys>Ala mutations in conserved RING domain
    Promoter
    EFS
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208047-plasmid-map-sequence-id-410205

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  11. pSLPB2B-ER50-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187948
    Purpose
    ER50 degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    ER50-SpdCas9-tagRFPt-P2A-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Mutation
    6 mutations, T371A, L384M, M421G, G521R, Y537S, N…
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187948-plasmid-map-sequence-id-373701

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  12. CA-RIT-NFAT1 (PGK-Puro)

    Plasmid
    #63214
    Purpose
    Constitutively active NFAT1, mutated to interfere selectively with the NFAT:AP-1 interactio. Contains Puromycin selection marker
    Depositor
    Anjana Rao
    Article
    Martinez et al Immunity. 2015 Feb 6. pii: S1074-7613(15
    Insert
    CA-RIT-NFAT1 (Nfatc2 Mouse)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Amino acids 1-3 removed; CA: PASSGSSASF mutated t…
    Available Since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. pSLPB2B-ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187950
    Purpose
    ecDHFR degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187950-plasmid-map-sequence-id-373501

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  15. pcDNA3-sACE2v2-sfGFP

    Plasmid
    #145173
    Purpose
    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 2
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available Since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145173-plasmid-map-sequence-id-292589

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  16. pdCas9-M-G6

    Plasmid
    #73437
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant G6.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor G6 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available Since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    73437-plasmid-map-sequence-id-135336

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  17. pcDNA3-sACE2v6-sfGFP

    Plasmid
    #145177
    Purpose
    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 6
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available Since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145177-plasmid-map-sequence-id-292606

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  18. pdCas9-M-3A2

    Plasmid
    #73435
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3A2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 3A2 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available Since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    73435-plasmid-map-sequence-id-135340

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  19. pcDNA3-sACE2v1-sfGFP

    Plasmid
    #145172
    Purpose
    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 1
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available Since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    145172-plasmid-map-sequence-id-292576

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  20. pCC_12 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139097
    Purpose
    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dxCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139097-plasmid-map-sequence-id-273896

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Showing: 4281 - 4300 of 4443 results