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Showing: 10751 - 10800 of 167282 results

  1. L0I75_tUBQ10_GG5-GG6

    Plasmid
    #219714
    Purpose
    Level 0 tUBQ10 terminator, GG5 and GG6 BsaI sites for golden gate assembly.
    Depositor
    Jennifer Nemhauser
    Insert
    GG5-tUBQ10-GG6
    Use
    Synthetic Biology
    Mutation
    WT terminator with added golden gate linkers
    Available Since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219714-plasmid-map-sequence-id-439236

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  2. pOCC102-MBP-eIF4G-SkA

    Plasmid
    #219977
    Purpose
    eIF4G-SkA protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4G-SkA (TIF4631 Budding Yeast)
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    eIF4A binding domain of S. kudriavzevii
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219977-plasmid-map-sequence-id-436800

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  3. pOCC102-MBP-eIF4G-RAR

    Plasmid
    #219973
    Purpose
    eIF4G-RAR protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4G-RAR (TIF4631 Budding Yeast)
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    Truncation: amino acids 490 to 942
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219973-plasmid-map-sequence-id-436750

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  4. pFHMSPN-avi-TEV-LIC V

    Plasmid
    #220885
    Purpose
    Donor vector to generate recombinant baculovirus by site-specific transposition in an E. coli host.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus expresssion
    Tags
    Honeybee melittin signal followed by His, avi and…
    Available Since
    June 20, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    220885-plasmid-map-sequence-id-436698

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  5. L0I89-pMUTE_GG0-1

    Plasmid
    #219708
    Purpose
    Level 0 pMUTE promoter, GG0 and GG1 BsaI sites for golden gate assembly.
    Depositor
    Jennifer Nemhauser
    Insert
    GG0-pMUTE-GG1
    Use
    Synthetic Biology
    Mutation
    WT promoter with added golden gate linkers
    Available Since
    June 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    219708-plasmid-map-sequence-id-435737

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  6. His-GST-MPK6

    Plasmid
    #215170
    Purpose
    Encodes the glutathionine S-transferase with N-terminal His tag and fused to the N-terminus of a mitogen-activated protein kinase 6 from Arabidopsis thaliana
    Depositor
    Travis Beddoe, Bhuvana Shanbhag
    Insert
    His-GST-MPK6
    Tags
    His-tag
    Expression
    Bacterial
    Promoter
    T7 lac
    Available Since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    215170-plasmid-map-sequence-id-430166

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  7. L0I74-_tUBQ10_GG4-GG6

    Plasmid
    #219713
    Purpose
    Level 0 tUBQ10 terminator, GG4 and GG6 BsaI sites for golden gate assembly.
    Depositor
    Jennifer Nemhauser
    Insert
    GG4-tUBQ10-GG6
    Use
    Synthetic Biology
    Mutation
    WT terminator with added golden gate linkers
    Available Since
    June 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    219713-plasmid-map-sequence-id-435864

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  8. L0I87-SPCHPromoter_GG0-1

    Plasmid
    #219707
    Purpose
    Level 0 pSPCH promoter, GG0 and GG1 BsaI sites for golden gate assembly.
    Depositor
    Jennifer Nemhauser
    Insert
    GG0-pSPCH-GG1
    Use
    Synthetic Biology
    Mutation
    WT promoter with added golden gate linkers
    Available Since
    June 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    219707-plasmid-map-sequence-id-435861

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  9. L0I80_Bxb1mut(noBsaI)_GG1-GG4

    Plasmid
    #219715
    Purpose
    Level 0 Bxb1 integrase with mutated BsaI sites, GG1 and GG4 BsaI sites for golden gate assembly.
    Depositor
    Jennifer Nemhauser
    Insert
    GG1-Bxb1(noBsaI0)-GG4
    Use
    Synthetic Biology
    Available Since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    219715-plasmid-map-sequence-id-435740

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  10. mPLD3-sgRNA-Cas9-mcherry

    Plasmid
    #199700
    Purpose
    encodes sgRNA for mouse PLD3 KO, (target 217-223aa) plus Cas9-P2A-mCherry
    Depositor
    David Nemazee
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    P2A-RFP
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available Since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    199700-plasmid-map-sequence-id-390617

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  11. MUT_S1-N

    Plasmid
    #218398
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, neutral region in second intron, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218398-plasmid-map-sequence-id-433922

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  12. MUT_N-H1

    Plasmid
    #218397
    Purpose
    3-exon minigene, with neutral middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218397-plasmid-map-sequence-id-433918

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  13. WT_S7-H1

    Plasmid
    #218393
    Purpose
    3-exon minigene, with SRSF7 motif-rich middle exon, and hnRNPA1 motif-rich region in second intron
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218393-plasmid-map-sequence-id-433906

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  14. pK2-J11

    Plasmid
    #218224
    Purpose
    Terminates AtU6-promoter driven sgRNA array with two guides, MoClo Compatible
    Depositor
    Daniel Voytas
    Insert
    linker sequence
    Use
    CRISPR
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218224-plasmid-map-sequence-id-431992

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  15. pR6K-GFP-CASTV

    Plasmid
    #199657
    Purpose
    R6K plasmid encoding a type V CAST but no CRISPR array
    Depositor
    IIya Finkelstein
    Insert
    CAST V systems
    Expression
    Bacterial
    Promoter
    J23119 promoter
    Available Since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    199657-plasmid-map-sequence-id-412267

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  16. pK4-J11

    Plasmid
    #218225
    Purpose
    Terminates AtU6-promoter driven sgRNA array with four guides, MoClo Compatible
    Depositor
    Daniel Voytas
    Insert
    linker sequence
    Use
    CRISPR
    Available Since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    218225-plasmid-map-sequence-id-431986

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  17. pK6-J11

    Plasmid
    #218226
    Purpose
    Terminates AtU6-promoter driven sgRNA array with tsix guides, MoClo Compatible
    Depositor
    Daniel Voytas
    Insert
    linker sequence
    Use
    CRISPR
    Available Since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    218226-plasmid-map-sequence-id-431985

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  18. pK8-J11

    Plasmid
    #218227
    Purpose
    Terminates AtU6-promoter driven sgRNA array with eight guides, MoClo Compatible
    Depositor
    Daniel Voytas
    Insert
    linker sequence
    Use
    CRISPR
    Available Since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    218227-plasmid-map-sequence-id-431982

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  19. pCLX-KRT7-GFP

    Plasmid
    #216853
    Purpose
    This plasmid is for the production of lentiviral vectors that can be used to identify and live sort pancreatic progenitor expresssing the Keratin7 gene.
    Depositor
    Patrick Salmon
    Insert
    GFP
    Use
    Lentiviral
    Promoter
    Keratin7
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216853-plasmid-map-sequence-id-431654

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  20. gWiz_BM40-vMAT1-VL-hIgG1-CL

    Plasmid
    #218441
    Purpose
    mammalian expression plasmid for the light chain fragment of an anti-vMAT1 Fab domain
    Depositor
    Axel Brunger
    Insert
    vMAT1-VL-hIgG1-CL
    Use
    Affinity Reagent/ Antibody
    Tags
    BM40
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    218441-plasmid-map-sequence-id-431103

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  21. pINER3R

    Plasmid
    #185883
    Purpose
    In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2)
    Expression
    Yeast
    Mutation
    None
    Available Since
    April 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    185883-plasmid-map-sequence-id-430023

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  22. pLSU1/t35s[TMV]:MCS+:NosT

    Plasmid
    #212160
    Purpose
    This binary vector has an empty backbone with extended multiple cloning site (MSC) where you can inserta a gene into a cassette with a strong promoter (truncated 35sCAMV) and UTR (Tomato Mosaic Virus)
    Depositor
    Wayne Curtis
    Type
    Empty backbone
    Expression
    Plant
    Available Since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    212160-plasmid-map-sequence-id-422551

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  23. pLSU1/t35S[TMV]:mNeonGreen:NosT

    Plasmid
    #212162
    Purpose
    This binary vector expresses mNeonGreen with a truncated 35sCAMV promoter with strong UTR (Tomato Mosaic Virus)
    Depositor
    Wayne Curtis
    Insert
    Tomato codon optimized version of mNeonGreen
    Expression
    Plant
    Mutation
    mNeonGreen codon-optimized for tomato
    Promoter
    truncated 35sCaMV
    Available Since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    212162-plasmid-map-sequence-id-422667

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  24. HA Display Part 1-4 DO

    Plasmid
    #206514
    Purpose
    CcdB Dropout vector for parts 1-4 of Hemagglutinin surface expression in mammalian cells
    Depositor
    IIya Finkelstein
    Type
    Empty backbone
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available Since
    Feb. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    206514-plasmid-map-sequence-id-424195

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  27. pCAG-ORF3cStrep

    Plasmid
    #208728
    Purpose
    Expresses ORF3c-Strep in mammalian cells
    Depositor
    Andrew Firth
    Insert
    ORF3c (ORF3a )
    Tags
    Strep
    Expression
    Mammalian
    Available Since
    Feb. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    208728-plasmid-map-sequence-id-420909

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  28. p_L_3c_SG_RLuc

    Plasmid
    #208729
    Purpose
    For assessing leaky scanning rates in mammalian cells
    Depositor
    Andrew Firth
    Insert
    ORF3c (ORF3a )
    Tags
    Renilla luciferase
    Expression
    Mammalian
    Available Since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    208729-plasmid-map-sequence-id-420912

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  29. pHflu3

    Plasmid
    #203881
    Purpose
    pHI1 with insertion of kanamycin-resistance gene
    Depositor
    Bogumil Karas
    Insert
    aminoglycoside O-phosphotransferase APH(3')-IIa
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    203881-plasmid-map-sequence-id-403829

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  30. pBP-P_TALEsp1_PUPsp1-lacI_Ptac-lacO

    Plasmid
    #204079
    Purpose
    Type 0 promoter part
    Depositor
    Mikhail Shapiro
    Insert
    TALEsp1-stabilized promoter driving lacI repressor; Ptac-lacO IPTG-inducible promoter
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204079-plasmid-map-sequence-id-413894

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  31. PTNA-c200

    Plasmid
    #205114
    Purpose
    PTN protein expression
    Depositor
    Paul Brennan
    Insert
    PTN (PTN Human)
    Tags
    AviTag-6His and PTPRSsp
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    205114-plasmid-map-sequence-id-411200

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  32. Kv1.3/RBG4

    Plasmid
    #196949
    Purpose
    Plasmid expressing an antigen targeting the Kv1.3 potassium channel subunit
    Depositor
    James Trimmer
    Insert
    Kv1.3 (Kcna3 Rat)
    Expression
    Mammalian
    Available Since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    196949-plasmid-map-sequence-id-388566

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  33. pCAGGS-iRFP713(C15S/V256C)-P2A-mCherry

    Plasmid
    #198069
    Purpose
    Mammalian expression of iRFP713(C15S/V256C) and EGFP
    Depositor
    Michiyuki Matsuda
    Insert
    iRFP713(C15S/V256C)
    Tags
    P2A-mCherry
    Expression
    Mammalian
    Mutation
    C15S/V256C mutation
    Promoter
    CAG
    Available Since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198069-plasmid-map-sequence-id-389877

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  34. pCSIIbleo-iRFP713(C15S/V256C)-P2A-EGFP

    Plasmid
    #198062
    Purpose
    The lentiviral vector for iRFP713(C15S/V256C) and EGFP
    Depositor
    Michiyuki Matsuda
    Insert
    iRFP713(C15S/V256C)
    Use
    Lentiviral
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    C15S/V256C mutation
    Promoter
    CAG
    Available Since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198062-plasmid-map-sequence-id-389830

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  35. pCSIIbleo-miRFP670nano(R57C/C86S)-P2A-EGFP

    Plasmid
    #197370
    Purpose
    The lentiviral vector for miRFP670nano(R57C/C86S) and EGFP
    Depositor
    Michiyuki Matsuda
    Insert
    miRFP670nano(R57C/C86S)
    Use
    Lentiviral
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    R57C/C86S mutation
    Promoter
    CAG
    Available Since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    197370-plasmid-map-sequence-id-389688

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  36. pCSIIbleo-6504-P2A-EGFP

    Plasmid
    #197368
    Purpose
    The lentiviral vector for NIR-GECOHI and EGFP
    Depositor
    Michiyuki Matsuda
    Insert
    NIR-GECO2(C18S/I426C)
    Use
    Lentiviral
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    C18S/I426C mutation
    Promoter
    CAG
    Available Since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    197368-plasmid-map-sequence-id-389693

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  40. pCI_E01_NtA-Fs1-mIgG1Fc-Myc-His-WPRE

    Plasmid
    #198134
    Purpose
    Express NtA-Fs1 in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    NtA-Fs1
    Tags
    mIgG1Fc, Myc, His
    Expression
    Mammalian
    Promoter
    CMV, beta-globin/IgG chimeric intron
    Available Since
    June 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    198134-plasmid-map-sequence-id-395024

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  42. pCEV-G2-Km ymTurquoise2

    Plasmid
    #193958
    Purpose
    TEF1 promoter controlled expression plasmid with G418 resistance for expressing ymTurquoise2 in budding yeast
    Depositor
    Bas Teusink
    Insert
    ymTurquoise2
    Expression
    Yeast
    Promoter
    TEF1
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    193958-plasmid-map-sequence-id-390052

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  43. pCI_E01_NtA-Fs1-Myc-His-WPRE

    Plasmid
    #198132
    Purpose
    Express NtA-Fs1 in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    NtA-Fs1
    Tags
    TEV-Myc-His
    Expression
    Mammalian
    Promoter
    CMV, beta-globin/IgG chimeric intron
    Available Since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198132-plasmid-map-sequence-id-389738

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  44. pLenti-hSyn1-ChR2(H134R)-EYFP

    Plasmid
    #197593
    Purpose
    Lentiviral transfer vector for the neuronal expression of ChR2(H134R)-EYFP (Channelrhodopsin for the inducing neuronal activity)
    Depositor
    Patrik Verstreken
    Insert
    ChR2(H134R)-EYFP
    Use
    Lentiviral
    Promoter
    hSynI
    Available Since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    197593-plasmid-map-sequence-id-389346

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  45. pQE80l B-CC-B

    Plasmid
    #196479
    Purpose
    Protein expression of SpyCatcher-ELP-CarHCC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCC
    Depositor
    Fei Sun
    Insert
    B-CC-B
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    196479-plasmid-map-sequence-id-387244

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  48. RENBP_11-427_BioN

    Plasmid
    #195869
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RENBP (RENBP Human)
    Use
    Pfbd-bira, baculovirus expression
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Promoter
    Polyhedrin
    Available Since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195869-plasmid-map-sequence-id-387258

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  49. NXPH1_22-271_AviN_HisC

    Plasmid
    #195872
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NXPH1 (NXPH1 Human)
    Use
    Pfhmsp-avin-lic-c, baculovirus expression
    Promoter
    Polyhedrin
    Available Since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195872-plasmid-map-sequence-id-387247

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  50. sfCherry11-Ex1-17-PTPN22

    Plasmid
    #195387
    Purpose
    Mammalian expression of a human PTPN22 mutant (exon 1-17) with sfCherry(11) tag
    Depositor
    Patrick Concannon
    Insert
    PTPN22 (PTPN22 Human)
    Tags
    sfCherry(11)
    Expression
    Mammalian
    Mutation
    PTPN22 mutant containing exon 1 to 17
    Available Since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195387-plasmid-map-sequence-id-387974

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Showing: 10751 - 10800 of 167282 results