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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 2,297 results for: pet28

Showing: 2161 - 2170 of 2297 results

  1. pJH859

    Plasmid
    #172177
    Purpose
    PylB overexpression and purification
    Depositor
    Matthew Bennett
    Article
    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:
    Insert
    PT7/Lac-[His6-TEV-SUMO-PylB.JM10.1deopt]
    Use
    Tags
    Expression
    Bacterial
    Mutation
    pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…
    Promoter
    Available since
    Sept. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172177-plasmid-map-sequence-id-338578

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  5. pLyGo-Ec-2

    Plasmid
    #163128
    Purpose
    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding MalE signal peptide and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    163128-plasmid-map-sequence-id-331492

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  7. pTH861-gst-optec P210T

    Plasmid
    #89595
    Purpose
    Plasmid for bacterial expression of a His-tagged P210T mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase P210T mutant, codon-optimised for bacterial expression
    Use
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed proline 210 to threonine
    Promoter
    T7
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89595-plasmid-map-sequence-id-206078

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  8. pTH860-gst-optec A180Q

    Plasmid
    #89594
    Purpose
    Plasmid for bacterial expression of a His-tagged A180Q mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase A180Q mutant, codon-optimised for bacterial expression
    Use
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed alanine 180 to glutamine
    Promoter
    T7
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89594-plasmid-map-sequence-id-206044

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  9. pTH858-gst-optec V156E

    Plasmid
    #89592
    Purpose
    Plasmid for bacterial expression of a His-tagged V156E mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase V156E mutant, codon-optimised for bacterial expression
    Use
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed valine 156 to glutamic acid
    Promoter
    T7
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89592-plasmid-map-sequence-id-206041

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  10. pTH859-gst-optec Y172M

    Plasmid
    #89593
    Purpose
    Plasmid for bacterial expression of a His-tagged Y172M mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase Y172M mutant, codon-optimised for bacterial expression
    Use
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed tyrosine 172 to methionine
    Promoter
    T7
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89593-plasmid-map-sequence-id-206040

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Showing: 2161 - 2170 of 2297 results