We narrowed to 22,830 results for: Sis

1. pHR_5xUAS-ETV2-P2A-BFP-WPRE_PGK-HygR

   Plasmid

   #216664

   Purpose

   Expressed Gal4-inducible ETV2 and BFP, with hygromycin resistance

   Depositor

   Leonardo Morsut

   Inserts

   ETV2 (Etv2 Mouse)
   BFP
   HygromycinR
   

   Use

   Lentiviral

   Available Since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3 Flag BimL

   Plasmid

   #24233

   Depositor

   Roger Davis

   Article

   Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

   Insert

   Bim L (BCL2L11 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   June 1, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pET-X(4130)B1

   Plasmid

   #168018

   Purpose

   Expresses X(4130)B1-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Blum et al Science. 2021 Feb 19;371(6531):803-810.

   Insert

   X(4130)B1-6xHis
   

   Tags

   Hisx6

   Expression

   Bacterial

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. GI-Gal4 DBD

   Plasmid

   #42500

   Purpose

   GIGANTEA fused with Gal4 DNA binding domain (DBD), part of a light-inducible transcription factor

   Depositor

   Ricardo Dolmetsch

   Article

   Yazawa et al Nat Biotechnol. 2009 Oct;27(10):941-5. d

   Insert

   GIGANTEA fused with Gal4 DBD
   

   Tags

   Gal4 DNA binding domain (DBD)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMA3287

   Plasmid

   #46883

   Purpose

   Lentiviral vector encoding Tet-inducible mitochondrially targeted Y147A mutant human Uracil-N-glycosylase

   Depositor

   Mikhail Alexeyev

   Article

   Shokolenko et al DNA Repair (Amst). 2013 Jul;12(7):488-99

   Insert

   Uracil-N-glycosylase, Y147A mutant (UNG Human)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Y147A

   Promoter

   TRE-Tight

   Available Since

   Aug. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMaCTag-P21

   Plasmid

   #120032

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 24xSunTag

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   24xSunTag
   

   Use

   Pcr template

   Tags

   24xSunTag

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMaCTag-P20

   Plasmid

   #120031

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 10xSuntag

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   10xSuntag
   

   Use

   Pcr template

   Tags

   10xSuntag

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCfB4906

   Plasmid

   #106150

   Purpose

   EasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying hygromycin resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Insert

   Cas9
   

   Expression

   Yeast

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSHDY-Prha-mVenus_rhaS

   Plasmid

   #137662

   Purpose

   Conjugative vector derived from pSHDY. Constitutive expression of the activator rhaS, as well as rhamnose-inducible expression of mVenus.

   Depositor

   Ilka Axmann

   Article

   Behle et al ACS Synth Biol. 2020 Apr 17;9(4):843-855

   Insert

   Prha:mVenus
   

   Expression

   Bacterial

   Mutation

   rhaS (Arg218Leu)

   Promoter

   Prha

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. p2T-CMV-EA-BE4max-NG-BlastR

    Plasmid

    #152992

    Purpose

    High purity C-to-T base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    BE4 H47E+S48A-nSpCas9-UGI-UGI
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMaCTag-P13

    Plasmid

    #120024

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mCherry
    

    Use

    Pcr template

    Tags

    mCherry

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBT346_pLambda-attB-pCA-GFP-pA

    Plasmid

    #52551

    Purpose

    for producing PhiC31 attB-containing minicircle in vitro

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    Lambda integrase attL1
    Lambda integrase attR1
    PhiC31o attB (full length)
    GFP
    

    Expression

    Mammalian

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-Tag1-NS5A

    Plasmid

    #17646

    Depositor

    Xin Wang

    Article

    Budhu et al Carcinogenesis. 2007 Jul . 28(7):1552-60

    Insert

    HCV NS5A (POLY Hepatitis C virus)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. APT3-Ykt6

    Plasmid

    #168003

    Purpose

    positive selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    Ykt6(L161-L177)-T3'PAP
    

    Use

    Synthetic Biology

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXPR_003 sgMSLN

    Plasmid

    #193588

    Purpose

    MSLN knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgMSLN (MSLN Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETSUMO-TtAgo

    Plasmid

    #210191

    Purpose

    Bacterial expression of the His- and SUMO-tagged Argonaute protein of Thermus thermophilus

    Depositor

    Phillip Zamore

    Article

    Smith et al Nat Commun. 2019 Jan 17;10(1):272. doi:

    Insert

    TtAgo (TT_P0026 Thermus thermophilus)
    

    Tags

    6x His tag and SUMO tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRGEB32-BAR

    Plasmid

    #126072

    Purpose

    For CRISPR/Cas9 delivery in maize. Expresses Cas9 with rice ubiquitin promoter, BAR with 35S promoter, and sgRNA/PTG with rice snoRNA U3 promoter (pol III). Can produce 1 or more gRNAs.

    Depositor

    Charles Hunter

    Article

    Hunter et al Plants (Basel). 2021 Apr 8;10(4). pii: p

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    35S for BAR; Rice UBI for Cas9; Rice snoRNA U3 fo…

    Available Since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. FSW-HRP-TM

    Plasmid

    #82540

    Purpose

    Lentiviral vector for expressing HRP-TM in neurons

    Depositor

    Alice Ting

    Article

    Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

    Insert

    HRP fused to TM domain of PDGFR
    

    Use

    Lentiviral

    Tags

    HRP

    Expression

    Mammalian

    Promoter

    Human Synapsin

    Available Since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. CRY2low-mCherry

    Plasmid

    #104065

    Purpose

    Expresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with mCherry

    Depositor

    Bianxiao Cui

    Article

    Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

    Insert

    CRY2low-mCherry
    

    Expression

    Mammalian

    Mutation

    CRY2 aa 1-491 truncation, R489E A491D

    Promoter

    CMV

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMaCTag-Z15

    Plasmid

    #120058

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with miRFP670

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    miRFP670
    

    Use

    Pcr template

    Tags

    miRFP670

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. YFP-HA-DAT

    Plasmid

    #90244

    Purpose

    Expresses YFP-HA-DAT in mammalian cells

    Depositor

    Alexander Sorkin

    Article

    Sorkina et al J Neurosci. 2006 Aug 2;26(31):8195-205.

    Insert

    DAT (dopamine transporter) (SLC6A3 Human)
    

    Expression

    Mammalian

    Mutation

    residues 193–203 of human DAT were replaced in YF…

    Promoter

    CMV

    Available Since

    June 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pMSCVhyg-3xT7-nSREBP1a

    Plasmid

    #171030

    Purpose

    Expresses 3xT7-tagged nuclear form of SREBP1a in mammalian cells

    Depositor

    Kai Ge

    Article

    Jang et al Genes Dev. 2021 Apr 22. pii: gad.347583.

    Insert

    nSREBP1a (Srebf1 Mouse)
    

    Use

    Retroviral

    Tags

    3xT7

    Expression

    Mammalian

    Mutation

    amino acids 1- 449

    Promoter

    5' LTR

    Available Since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. pMaCTag-Z08

    Plasmid

    #120051

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mClover3

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mClover3
    

    Use

    Pcr template

    Tags

    mClover3

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET-BoNT/X

    Plasmid

    #168017

    Purpose

    Expresses BoNT/X-LC(1-439)-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    BoNT/X-LC(1-439)-6xHis
    

    Tags

    Hisx6

    Expression

    Bacterial

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pSF280

    Plasmid

    #167675

    Purpose

    Intermediate vector for six gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. OSCA1.2-peGFP-N1

    Plasmid

    #228585

    Purpose

    Expresses OSCA1.2-eGFP in mammalian cells

    Depositor

    Huanghe Yang

    Article

    Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

    Insert

    hyperosmolality-gated Ca2+ permeable channel (OSCA) 1.2 (AT4G22120 Mustard Weed)
    

    Expression

    Mammalian

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. pCB6-HFE-EGFP

    Plasmid

    #12104

    Depositor

    Pamela Bjorkman

    Article

    Giannetti et al J Biol Chem. 2004 Jun 11. 279(24):25866-

    Insert

    HFE (HFE Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

28. pLminP_Luc2P_RE45

    Plasmid

    #90383

    Purpose

    EGR1 - Early growth response factor gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Promoter

    EGR1

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEGFP-C2 Chfr (1014)

    Plasmid

    #61853

    Purpose

    mammalian expresion of Chfr fused to EGFP

    Depositor

    Jonathon Pines

    Article

    Matsusaka et al J Cell Biol. 2004 Aug 16;166(4):507-16.

    Insert

    Chfr (CHFR Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

30. puc19-pCas13a

    Plasmid

    #118963

    Purpose

    Intermediate/cloning vector that can be used for sub-cloning into final destination vectors based on one's requirements.

    Depositor

    Magdy Mahfouz

    Article

    Aman et al Genome Biol. 2018 Jan 4;19(1):1. doi: 10

    Insert

    Cas13a
    

    Expression

    Plant

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMaCTag-Z12

    Plasmid

    #120055

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mScarlet

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mScarlet
    

    Use

    Pcr template

    Tags

    mScarlet

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGilesSHdI_redgreen_2395A_His6_triple red_pLeft1806_His6_double green

    Plasmid

    #170048

    Purpose

    Tri-Responsive Reporter Gene Construct for redgreen, triple red, and double green

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    mScarlet
    COmScarlet
    mRuby3
    mWasabi dN
    mWasabi CO
    

    Use

    Unspecified

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSJ2039 (pRS316-NOP1pr-GFP1-10-SCS2TM)

    Plasmid

    #86418

    Purpose

    ONM/ER split GFP1-10

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    NOP1pr-GFP1-10-Scs2TM
    

    Tags

    GFP1-10

    Expression

    Yeast

    Promoter

    NOP1

    Available Since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV-Tag1-NS3

    Plasmid

    #17645

    Depositor

    Xin Wang

    Article

    Budhu et al Carcinogenesis. 2007 Jul . 28(7):1552-60

    Insert

    HCV NS3 (POLY Hepatitis C virus)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAC-94N

    Plasmid

    #53281

    Purpose

    Contains crtI, crtY, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and produces beta-carotene in E. coli when complemented with a gene encoding geranylgeranyl diphosphate synthase

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    crtY, crtI, crtB
    

    Use

    Low copy number bacterial cloning vector

    Promoter

    endogenous promoters

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. FU- dio Teal-gephyrin - W

    Plasmid

    #73918

    Purpose

    To express the inhibitory scaffold gephyrin tagged to teal fluorescent protein. Expression is Cre dependant. Plasmid can also be used to make lentivirus.

    Depositor

    Elly Nedivi

    Article

    Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

    Insert

    Teal-gephyrin
    

    Use

    Lentiviral

    Tags

    Teal

    Expression

    Mammalian

    Available Since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAC-PHYTipi

    Plasmid

    #53283

    Purpose

    Contains crtE, crtB, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces phytoene in E. coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    crtE, idi, crtY, crtI, crtB
    

    Use

    Low copy number bacterial cloning vector

    Promoter

    endogenous promoters

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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38. 5aa SunTag VP64 CLV3

    Plasmid

    #115485

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to the CLV3 locus with two guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pHR_dSV40-Aurora A-GFP

    Plasmid

    #67924

    Purpose

    Expresses Aurora A-GFP in mammalian cells

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

    Insert

    GFP-Aurora A (AURKA Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. 5aa SunTag VP64 AP3

    Plasmid

    #115484

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to the AP3 locus with two guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge