We narrowed to 9,801 results for: pET

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pET28-hisSumo-KE-Des27.13

Plasmid

#248008

Purpose

Expression of synthetic Kemp eliminase (Des27.13)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.13

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-mNeptune2.5

Plasmid

#243765

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mNeptune2.5. VHH-mNeptune2.5 also contains a T7, HA, BAP and His6 epitope

Depositor

Dominik Buser, Martin Spiess

Article

Buser et al J Vis Exp. 2025 Oct 17;(224). doi: 10.37

Insert

anti-GFP nanobody VHH

Tags

T7-TEV site and mNeptune2.5-HA-AviTag-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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IQ-compete reporter (episomal)

Plasmid

#247217

Purpose

Single-copy plasmid expressing the IQ-compete reporter (GFP-TCS-Quencher-CL1).

Depositor

Johannes Herrmann

Article

Hoffman et al FEBS Lett. 2025 Oct 25. doi: 10.1002/187

Insert

GFP-TCS-Quencher-CL1

Expression

Yeast

Promoter

TEF1

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-mCardinal2

Plasmid

#243766

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCardinal2. VHH-mCardinal2 also contains a T7, HA, BAP and His6 epitope

Depositor

Dominik Buser, Martin Spiess

Article

Buser et al J Vis Exp. 2025 Oct 17;(224). doi: 10.37

Insert

anti-GFP nanobody VHH

Tags

T7-TEV site and mCardinal2-HA-AviTag-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-iRFP670

Plasmid

#243768

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to iRFP670. VHH-iRFP670 also contains a T7, HA, BAP and His6 epitope

Depositor

Dominik Buser, Martin Spiess

Article

Buser et al J Vis Exp. 2025 Oct 17;(224). doi: 10.37

Insert

anti-GFP nanobody VHH

Tags

T7-TEV site and iRFP670-HA-AviTag-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pET32a-Nb19-P4S

Plasmid

#236500

Purpose

Expresses anti-Apolipoprotein A1 nanobody Nb19 as a fusion protein with coiled coil P4S in bacteria.

Depositor

Alexander Green

Article

Kang et al ACS Synth Biol. 2025 Sep 3. doi: 10.1021

Insert

Nb19-P4S

Expression

Bacterial

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pET32a-301-P3S

Plasmid

#236498

Purpose

Expresses anti-RiVax nanobody 301 as a fusion protein with coiled coil P3S in bacteria.

Depositor

Alexander Green

Article

Kang et al ACS Synth Biol. 2025 Sep 3. doi: 10.1021

Insert

301-P3S

Expression

Bacterial

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pETA_21 tRNA genes

Plasmid

#242431

Purpose

Expresses 21 tRNAs. The plasmid contains a T7 promoter upstream and an Nt.BspQI recognition site as well as a T7 terminator downstream of each tRNA gene.

Depositor

Sebastian Maerkl

Article

Li et al Nat Commun. 2025 Jul 5;16(1):6212. doi:

Insert

21 tRNA genes

Expression

Bacterial

Available Since

Aug. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pET50del_SynPspA_R44K/E126Q/E179Q

Plasmid

#223819

Purpose

Bacterial expression of SynPspA R44K/E126Q/E179Q with N-terminal 6xHis-Tag and 3C cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Nat Struct Mol Biol. 2025 Jan;32(1):23-3

Insert

SynPspA

Tags

6xHis

Expression

Bacterial

Mutation

R44K, E126Q, E179Q

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-His6-UROD

Plasmid

#236739

Purpose

Protein overexpression of human uroporphyrinogen decarboxylase

Depositor

Christina Woo

Article

Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

Insert

Uroporphyrinogen decarboxylase human (UROD Human)

Tags

His6

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET15b-NHis6-NocL

Plasmid

#240398

Purpose

expresses N-His tagged NocL (fatty acid activating ligase) from Nodularia sp. 06071

Depositor

Emily Balskus

Article

Wang et al Biochemistry. 2025 May 20;64(10):2173-21

Insert

nocL(fatty acyl-AMP ligase)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a:His-MBP-PKD

Plasmid

#237521

Purpose

Recombinant protein expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4_pseudokinase

Expression

Bacterial

Mutation

Pseudokinase region of RWT4

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a:His-MBP-RWT4Ca

Plasmid

#237518

Purpose

Recombinant protein expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca

Expression

Bacterial

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a:His-MBP-RWT4Ca_noKDup

Plasmid

#237535

Purpose

Recombinant protein expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca_noKDup

Expression

Bacterial

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pEt28a-SilkTag-CBM-H6

Plasmid

#230903

Purpose

Bacterial expression of SilkTag

Depositor

Sesilja Aranko

Article

Fan et al Angew Chem Int Ed Engl. 2023 Mar 6;62(11

Insert

SilkTag-CBM-H6

Tags

HisTag

Expression

Bacterial

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pEt28a-H6-SUMO-SilkCatcher

Plasmid

#230902

Purpose

Bacterial expression of SilkCatcher

Depositor

Sesilja Aranko

Article

Fan et al Angew Chem Int Ed Engl. 2023 Mar 6;62(11

Insert

H6-SUMO-SilkCatcher

Tags

HisTag, thrombin, SUMO

Expression

Bacterial

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-Pchi-HMG

Plasmid

#231540

Purpose

Expresses the HMG of Pchi Sox with a 6xHis tag for protein purification

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Sox HMG

Tags

6xHis

Expression

Bacterial

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-Salhel-HMG

Plasmid

#231539

Purpose

Expresses the HMG of Salhel Sox-I with a 6xHis tag for protein purification

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Sox-I HMG

Tags

6xHis

Expression

Bacterial

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-Myflu-POU

Plasmid

#231542

Purpose

Expresses the DNA binding domain of Myflu POU with a 6xHis tag for protein purification

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

POU DBD

Tags

6xHis

Expression

Bacterial

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-Salhel-POU

Plasmid

#231541

Purpose

Expresses the DNA binding domain of Salhel POU with a 6xHis tag for protein purification

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

POU DBD

Tags

6xHis

Expression

Bacterial

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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