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pICE-HA-CtIP-siR-S664A-S679A-S745A

Plasmid

#82031

Purpose

Plasmid for constitutive or doxy-inducible expression of S664A+S679A+S745A human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Sébastien Britton, Patrick Calsou

Article

Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

Insert

CtIP (RBBP8 Human)

Tags

HA

Expression

Mammalian

Mutation

Non phosphorylatable mutations on 3 of the main A…

Promoter

CMV-tet

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SNORD27h_2ntmut

Plasmid

#73067

Purpose

expression clone for human mutated SNORD27 (C/D box snoRNA U27)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD27 (E2F7 Human)

Tags

no tag

Expression

Mammalian

Mutation

2 nt mutation in AS box that binds E2F7 pre-mRNA

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pCMV-myc-Sema4D-CD4-FLAG

Plasmid

#51601

Purpose

expresses non-cleavable Sema4D/CD4 chimera with extracellular Sema4D, intracellular CD4

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4D/CD4-FLAG (Sema4d Mouse, Human)

Tags

FLAG and myc

Expression

Mammalian

Mutation

Sema4D amino acids 660-861 were replaced with CD4…

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_hE2F7mg_PP7-tag

Plasmid

#73078

Purpose

human E2F7 minigene (exons 11,12,13/introns cassette) with PP7-tag on 5'-end

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

E2F7 (E2F7 Human)

Tags

5'-PP7-tag RNA

Expression

Mammalian

Mutation

partial gene, exons 11,12,13 spaced by introns

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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3159_pETcon-SARS-CoV-2-RBD-L452R-T478K

Plasmid

#184407

Purpose

yeast surface display of the SARS-CoV-2 Delta variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Delta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

L452R+T478K

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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-161+80pCalb2/E2F2/NRF-1(-64)-like

Plasmid

#66742

Purpose

luciferase reporter for Calb2 promoter (-161to +80, mutant)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp+80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CGCG(-64) to TACA

Promoter

Calb2

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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161+80pCalb2/mutCREB(-2)-like

Plasmid

#66751

Purpose

luciferase reporter for Calb2 promoter (-161to +80, mutant)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp +80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

GCGC (-2) to TAGA

Promoter

Calb2

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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-161+80pCalb2/mutNRF-1(-41)-like

Plasmid

#66744

Purpose

luciferase reporter for Calb2 promoter (-161to +80, mutant)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp+80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CGC (-41) to ATT

Promoter

Calb2

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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-161+80pCalb2/dmutNRF-1(-41;-35)-like

Plasmid

#66741

Purpose

luciferase reporter for Calb2 promoter (-161to +80, mutant)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp+80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CGCAGGCGC (-41;-35) to ATTAGGATT

Promoter

Calb2

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_hE2F7mg_2ntmut

Plasmid

#73074

Purpose

human E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

E2F7 (E2F7 Human)

Expression

Mammalian

Mutation

partial gene, exons 11,12,13 spaced by introns, 2…

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA5-3xFLAG-NRF1-FL_WobbleMut

Plasmid

#237455

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged full-length NRF1 isoform mutated to be resistant to NRF1 siRNA

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

NRF1 full length

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA5-3xFLAG-NRF1-∆ex7_WobbleMut

Plasmid

#237456

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged NRF1 isoform lacking the alternative exon-7 (∆ex7) mutated to be resistant to NRF1 siRNA

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

NRF1 ∆exon-7

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-RVdG-MCP:oScarlet:KASH-6xMBS

Plasmid

#231011

Purpose

CVS N2c RVdG genome plasmid. Utilizes MS2 tagging to deliver the MCP:oScarlet:KASH transcript to the outer nuclear membrane. Insert high complexity barcode between SacII RE sites distal to 6xMBS site.

Depositor

Tomasz Nowakowski

Article

Shin et al bioRxiv [Preprint]. 2024 Dec 11:2024.10.

Insert

MCP-oScarlet-KASH-6xMBS-SacIIMCS

Use

Neurotropic virus

Tags

KASH and MCP

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pJL388

Plasmid

#243717

Purpose

Stably expresses a Myc-tagged AKAP1 with a GDDG mutation and gRNA-resistant silent mutations

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

AKAP1 (AKAP1 Human)

Use

Lentiviral

Tags

Myc

Mutation

GXXG motif (GKQG 624-627) mutated to GDDG, and gR…

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBD-PYR1

Plasmid

#241278

Purpose

Yeast two hybrid vector expressing BD-PYR1 (wild type)

Depositor

Sean Cutler

Article

Park et al Science. 2009 May 22;324(5930):1068-71.

Insert

PYR1 (PYR1 Mustard Weed)

Expression

Yeast

Mutation

NA

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pLex307-UCOE-SFFV-puroR-MCP-MLLx3-HSF1

Plasmid

#218558

Purpose

Encodes SFFV promoter-driven MCP-MLLx3-HSF1 with an N-terminal bipartite SV40 NLS, along with puromycin resistance

Depositor

Alejandro Chavez

Article

Giddins et al Nat Commun. 2025 Nov 20;16(1):11114. doi

Insert

MCP-MLLx3-HSF1

Use

Lentiviral

Tags

SV40NLS

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pLex307-UCOE-SFFV-puroR-MCP-P65-HSF1

Plasmid

#218559

Purpose

Encodes SFFV promoter-driven MCP-P65-HSF1 with an N-terminal bipartite SV40 NLS, along with puromycin resistance

Depositor

Alejandro Chavez

Article

Giddins et al Nat Commun. 2025 Nov 20;16(1):11114. doi

Insert

MCP-P65-HSF1

Use

Lentiviral

Tags

SV40NLS

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA377 - pBA904 Puro-T2A-GFP CD55 CRISPRa guide 1 (pRCA360 backbone)

Plasmid

#238168

Purpose

Lentiviral CRISPR guide vector expressing CD55 targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

CD55 sgRNA CRISPRa (CD55 Synthetic, Human)

Use

CRISPR and Lentiviral

Tags

GFP

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA810 - pBA904 Puro-T2A-GFP HES7 g2 CRISPRa guide (pRCA360 backbone) 674

Plasmid

#238177

Purpose

Lentiviral CRISPR guide vector expressing a HES7 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

HES7 sgRNA CRISPRa (HES7 Synthetic, Human)

Use

CRISPR and Lentiviral

Tags

GFP

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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R2pH-LAMP-FRB

Plasmid

#225138

Purpose

R2pH-LAMP1-labelled lysosomes with FRB

Depositor

Massimiliano Stagi

Article

Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

Insert

Lamp1 (Lamp1 Mouse)

Expression

Mammalian

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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