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pYC119

Plasmid

#63916

Purpose

Recipient overexpressing vector to construct FLAG tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

FLAG

Expression

Yeast

Promoter

TEF1 (Saccharomyces cerevisiae)

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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pIF251

Plasmid

#133935

Purpose

Kanamycin selection marker flanked by ~200 bp of homology to bacteriophage lambda. The selection marker will recombineer 21.3-22.8 kb away from the cosL DNA end of the phage genome.

Depositor

IIya Finkelstein

Article

Kim et al Sci Rep. 2017 May 18;7(1):2071. doi: 10.

Insert

Kanamycin selection marker flanked by ~200 bp of homology to bacteriophage lambda

Use

Λ-phage recombineering plasmid

Available Since

March 3, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pTol2_(-3kb)nrg1_typeII:GFP-CAAX

Plasmid

#199394

Purpose

pDEL63; transgenic construct to express GFP-CAAX under nrg1 type II regulatory region control

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

(-3kb)nrg1 type II
GFP-CAAX
afp_3'_UTR

Use

Tol2 gateway destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAIO-AsCpf1

Plasmid

#137846

Purpose

AsCpf1 and gRNA expression plasmid for P. falciparum; no selection in parasites.

Depositor

Josh Beck

Article

Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

Insert

AsCpf1-NLS-FLAG

Use

CRISPR

Available Since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pOC4 Actb-mRuby3 KI

Plasmid

#183437

Purpose

FlpON knock-in for mRuby3-beta-actin (amino acid position: before start codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and mRuby3 donor

Use

CRISPR

Expression

Mammalian

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pOC5 Tubb3-GFP KI

Plasmid

#183440

Purpose

FlpOFF knock-in for beta3-tubulin GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP donor

Use

CRISPR

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGs-Hoxd10

Plasmid

#45721

Depositor

Jeremy Dasen

Article

Dasen et al Cell. 2008 Jul 25;134(2):304-16. doi: 10

Insert

Hoxd10 (HOXD10 Chicken)

Promoter

CMV / b-actin

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only
MSGV Rh Acceptor PGK-NGFR

Plasmid

#64272

Purpose

Retroviral golden gate cloning vector for expression of functional Rhesus T-Cell Receptor genes with NGFR

Depositor

David Ott

Article

Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

Type

Empty backbone

Use

Retroviral

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pYC44

Plasmid

#63903

Purpose

Initial Integrative Vector

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

FRT::3'UTRcta::NAT::FRT

Expression

Yeast

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-cfRab11a_2

Plasmid

#26710

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rab11a RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Rab11a RNAi.

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro-cfPar3_4

Plasmid

#26706

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Dog Par3 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Par3 RNAi

Available Since

Jan. 3, 2011

Availability

Academic Institutions and Nonprofits only
pORANGE Tubb3-4xGFP KI

Plasmid

#183445

Purpose

GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and 4xGFP donor

Use

CRISPR

Expression

Mammalian

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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FT1

Plasmid

#31435

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Fused Toes Homolog (AKTIP Human)

Tags

HA

Expression

Yeast

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pLKO.1-Blast-cfCdc42_2

Plasmid

#26703

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Cdc42

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

RNAi to canine Cdc42

Available Since

Dec. 14, 2010

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro-cfRab8a_2

Plasmid

#26707

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rab8a RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Rab8a RNAi.

Available Since

Dec. 14, 2010

Availability

Academic Institutions and Nonprofits only
pBN79

Plasmid

#86717

Purpose

DamID plasmid for C. elegans

Depositor

Peter Askjaer

Article

Gonzalez-Aguilera et al Genome Biol. 2014 Feb 3;15(2):R21. doi:

Insert

Phsp16.41::Dam::MYC::Emr-1

Expression

Worm

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pML1

Plasmid

#108332

Purpose

MIDAS Level-1 source vector for cloning of modules (synthetic biology parts)

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pYC64

Plasmid

#63928

Purpose

Recipient replicative vector to construct FLAG tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

FLAG

Expression

Yeast

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pYC62

Plasmid

#63927

Purpose

Recipient replicative vector to construct HA tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

HA

Expression

Yeast

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Blast-cfCdc42_3

Plasmid

#26704

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Dog Cdc42 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Cdc42 RNAi

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only