We narrowed to 11,278 results for: VARS

1. CoV2-N-R203M

   Plasmid

   #177952

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203M). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (R203M) (N )
   

   Expression

   Mammalian

   Mutation

   R203M

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGPTVII-SED1

   Plasmid

   #232615

   Purpose

   SED1 biosensor for expression in Arabidopsis thaliana

   Depositor

   Jose Dinneny

   Article

   Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

   Insert

   SED1 Biosensor
   

   Expression

   Plant

   Promoter

   pUBQ10

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDV06.2c_DART-VADAR_iRFP720-sensor

   Plasmid

   #200410

   Purpose

   pDV06 with iRFP720-targeted sensor sequence inserted. Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon detection of the mRNA produced by pDV05.0, pDV05.1 and pDV05.2

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   Closed-loop DART VADAR sensor detecting iRFP720
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO-CreERT2 stuffer v3

   Plasmid

   #158046

   Purpose

   lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Oct. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. CBE-T1.46

   Plasmid

   #193284

   Purpose

   Expression of CBE-T1.46 base editor in mammalian cells, CMV promoter

   Depositor

   Nicole Gaudelli

   Article

   Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   CBE-T1.46
   

   Expression

   Mammalian

   Mutation

   D10A (Cas9), TadA mutations within

   Promoter

   CMV

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pV1393

   Plasmid

   #111430

   Purpose

   CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. CoV2-N-WT-Hu1

   Plasmid

   #177937

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence) (N )
   

   Expression

   Mammalian

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. SF-U7snRNA-CypA-E4

   Plasmid

   #190695

   Purpose

   Control vector expressing a U7-based antisense oligonucleotide targeting CypA exon 4

   Depositor

   Pim Pijnappel

   Article

   van der Wal et al Mol Ther Nucleic Acids. 2017 Jun 16;7:90

   Insert

   U7 snRNA antisense CypA Exon 4
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U7 promoter

   Available Since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-ABEmax-NRTH

   Plasmid

   #136922

   Purpose

   Expresses ABEmax-NRTH in mammalian cells

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   ABEmax-NRTH
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   see manuscript

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA5/FRT/TO_CEP41-HaloTag7_T2A_EGFP

    Plasmid

    #169327

    Purpose

    Expression of CEP41-HaloTag7 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    CEP41-HaloTag7 (CEP41 Human)
    

    Tags

    HaloTag7

    Expression

    Mammalian

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHH0103_YES1-1/1

    Plasmid

    #91439

    Purpose

    Protein expression and purification of human SH3 domain construct YES1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    YES1-1/1 (YES1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-ABEmax-NRCH

    Plasmid

    #136923

    Purpose

    Expresses ABEmax-NRCH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    ABEmax-NRCH
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. dxCas(3.7)-VPR

    Plasmid

    #108383

    Purpose

    Mammalian expression vector for xCas9 3.7 with VP64-p65-Rta (VPR) fused to its C-terminus

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    dxCas(3.7)-VPR
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-U6-DR30(SapI)-EFS-CasRx-pA

    Plasmid

    #192488

    Purpose

    To express CasRX and a gRNA

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    CasRx
    

    Use

    AAV

    Mutation

    No

    Promoter

    EFS

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. p2,0_3M5M_eGFP

    Plasmid

    #135474

    Purpose

    Encodes Marburg virus minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoter

    Depositor

    Elke Mühlberger

    Article

    Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

    Insert

    Marburg virus minigenome, eGFP reporter
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    T7

    Available Since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL4_10xUAS-MLPmin-luc2

    Plasmid

    #194383

    Purpose

    Reporter plasmid for split TEV assays; firefly luciferase reporter gene driven by 10x clustered UAS binding sites linked to a minimal MLP promoter

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    10xUAS-luc2
    

    Expression

    Mammalian

    Promoter

    MLP minimal

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. BB44_pmc.DonorR5.TS

    Plasmid

    #199223

    Purpose

    Donor plasmid with CCR5 target sites for ITPN or HMEJ knock-in at the human CCR5 safe harbour locus

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

    Insert

    Expression unit for mCherry - monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)
    

    Use

    Gene targeting donor plasmid

    Expression

    Mammalian

    Promoter

    Human PGK1 gene promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. eSpCas9(1.1)

    Plasmid

    #138555

    Purpose

    Expresses human codon-optimized enhanced specificity SpCas9 and blasticidin resistance: EFS promoter-eSpCas9(1.1)-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    eSpCas9(1.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    K848A, K1003A, R1060A

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. CBE-T1.52

    Plasmid

    #193285

    Purpose

    Expression of CBE-T1.52 base editor in mammalian cells, CMV promoter

    Depositor

    Nicole Gaudelli

    Article

    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    CBE-T1.52
    

    Expression

    Mammalian

    Mutation

    D10A (Cas9), TadA mutations within

    Promoter

    CMV

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. His-PIMT

    Plasmid

    #34852

    Depositor

    Bruce Downie

    Article

    Dinkins et al Plant J. 2008 Jul;55(1):1-13.

    Insert

    PIMT (PCMT1 Human)
    

    Tags

    6XHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 24, 2012

    Availability

    Academic Institutions and Nonprofits only