We narrowed to 4,942 results for: puc

1. PB-TO-NGN2-EMX1 BSD-mApple

   Plasmid

   #182311

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 and EMX1 expression, bsd selection, mApple

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Inserts

   hNGN2-P2A-EMX1 (NEUROG2, EMX1 Human)
   bsd-T2A-mycNLS-mApple
   

   Use

   Tags

   T2A-mycNLS-mApple

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and TRE3G

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEMS2232

   Plasmid

   #158401

   Purpose

   High Copy number plasmid containing Ple316 (NR2E1 MiniPromoter) insert

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

   Insert

   Ple316 (NR2E1 Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Ple316

   Available since

   Feb. 4, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. FASTHDR-Cterm-HaloTag-Puromycin

   Plasmid

   #167208

   Purpose

   Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with Puromycin

   Depositor

   Oscar Perez

   Article

   Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKL123

   Plasmid

   #187889

   Purpose

   AAVS1 safe-harbor integration of dox-inducible NFIA-SOX9

   Depositor

   Martin Kampmann

   Article

   Leng et al Nat Neurosci. 2022 Nov;25(11):1528-1542.

   Insert

   NFIA-SOX9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. FASTHDR-Cterm-mRuby3-Zeo

   Plasmid

   #167206

   Purpose

   Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mRuby3 and selection with Zeocin

   Depositor

   Oscar Perez

   Article

   Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   mRuby3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-C-miRFP670nano3-Blast-H2BC11

   Plasmid

   #207762

   Purpose

   Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a miRFP670nano3-Blast Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. qTAG-C-mScarlet-Puro-H2BC11

   Plasmid

   #207761

   Purpose

   Donor template for mScarlet-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a mScarlet-Puro Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 17, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. FASTHDR-Cterm-mTagBFP2-Blasticidin

   Plasmid

   #167207

   Purpose

   Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mTagBFP2 and selection with Blasticidin

   Depositor

   Oscar Perez

   Article

   Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-C-mStayGold-Puro-PXN

   Plasmid

   #227317

   Purpose

   Donor template for mStayGold-2A-Puro insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   PXN Homology Arms flanking a mStayGold-2A-Puro Cassette (PXN Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-C-moxGFP-Zeo-H2BC11

    Plasmid

    #207759

    Purpose

    Donor template for moxGFP-2A-Zeo insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a moxGFP-Zeo Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pGGD_MiniTurboID-YFP

    Plasmid

    #222434

    Purpose

    Golden Gate / Green Gate module for adding MiniTurboID and YFP as C-terminus of target protein.

    Depositor

    Dominique Bergmann

    Article

    Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.

    Insert

    MiniTurboID (BirA mutant)
    

    Use

    Golden gate / green gate compatible cloning vector

    Tags

    Linker and YFP

    Expression

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT899

    Plasmid

    #228288

    Purpose

    Constitutive mutant rPhlTA expression with strong promoter

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    rphlTA mutant
    

    Use

    Synthetic Biology

    Tags

    Three copies of VP16 (VP48)-Nuclear localization …

    Expression

    Yeast

    Mutation

    P5S, S6P, K86T, F109L, Q117R, E143K

    Promoter

    KpGAPDH promoter

    Available since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. TRAV26-2

    Plasmid

    #123399

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRAV26-2 (TRAV26-2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGGB_YFP-MiniTurboID

    Plasmid

    #222433

    Purpose

    Golden Gate / Green Gate module for adding YFP and MiniTurboID as N-terminus of target protein.

    Depositor

    Dominique Bergmann

    Article

    Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.

    Insert

    MiniTurboID (BirA mutant)
    

    Use

    Golden gate / green gate compatible cloning vector

    Tags

    Linker and YFP

    Expression

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. TRBV6-5

    Plasmid

    #123425

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRBV6-5 (TRBV6-5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAVS1-shRNA

    Plasmid

    #82697

    Purpose

    For targeting shRNA construct into human AAVS1 locus, using genome editing. Expresses shRNA of interest (cloning: EcoRI and AgeI) under U6 promoter, flanked by AAVS1 homology arms.

    Depositor

    Alan Mullen

    Article

    Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

    Type

    Empty backbone

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    hPGK, U6

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSL5069 (pDonor(AAVS1),CRISPR_Pse)

    Plasmid

    #200904

    Purpose

    Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a splice acceptor-T2A-PuroR-T2A-eGFP cargo, and a CRISPR RNA under a U6 promoter. Total transposon size = 2,024 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    Mini Tn7016 (AAVS1 NGS), PseCRISPR (tSL425)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNKX2-5-T2A-GFP-PGK-Puro

    Plasmid

    #203607

    Purpose

    Donor plasmid to create 3' knockin of NKX2-5 with T2A-GFP

    Depositor

    Matthew Birket

    Article

    Douglas et al Stem Cell Res. 2023 Dec 3;74:103262. doi

    Insert

    NKX2-5-T2A-GFP-PGK-Puro-NKX2-5 (NKX2-5 Human)
    

    Use

    Human targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. CLYBL-TO-PAX7

    Plasmid

    #204346

    Purpose

    Knock-in vector to insert a dox-inducible PAX7 transgene into the CYBL locus in human pluripotent stem cells. PAX7 induction results in myogenic differentiation.

    Depositor

    Ivo Lieberam

    Article

    Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

    Insert

    human PAX7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSKC12 - DelQ

    Plasmid

    #110493

    Purpose

    Monitor the expression of Luciferase driven by a mutated NRAS 5'-UTR.

    Depositor

    Shankar Balasubramanian

    Article

    Kumari et al Nat Chem Biol. 2007 Apr;3(4):218-21. doi

    Insert

    NRAS 5'- DelQ (bp 30-254) (NRAS Human)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mutation

    Deletion of the first 29 base pairs of the NRAS 5…

    Promoter

    T7

    Available since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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