We narrowed to 18,681 results for: tec

1. pRAM28

   Plasmid

   #234314

   Purpose

   pColE1 origin Cat-RNA U1376, Multiplexing ribozyme splicing reactions for the purpose of comparing host range of origins of replications

   Depositor

   James Chappell

   Article

   Kalvapalle et al Nat Biotechnol. 2025 Mar 18. doi: 10.103

   Insert

   U1367 Targeting RAM, Barcode 2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   P. CymRC

   Available since

   March 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. mRNA-CleanCap entry vector

   Plasmid

   #188249

   Purpose

   Cloning vector for generating template plasmids to transcribe mRNA, with mNeonGreen dropout following BsaI digestion

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. VRQR-ABEmax

   Plasmid

   #119811

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   ecTadA-ecTadA*(7.10)-nSpCas9 (VRQR)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pICH86966::AtU6p::sgRNA_PDS

   Plasmid

   #46966

   Purpose

   Expresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoter

   Depositor

   Sophien Kamoun

   Article

   Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

   Insert

   AtU6p::sgRNA_PDS
   

   Use

   CRISPR; Plant expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. p201N Cas9

   Plasmid

   #59175

   Purpose

   Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

   Depositor

   Wayne Parrott

   Article

   Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

   Inserts

   Cas9
   nptII
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   2x35S and StUbi-3P

   Available since

   Sept. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGGF_RT_FAST_RFP

   Plasmid

   #136979

   Purpose

   Goldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescence

   Depositor

   Klaus Theres

   Article

   Rossmann et al Plant J. 2019 Oct 30. doi: 10.1111/tpj.1

   Insert

   seed coat marker RFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   BsaI site domestication in pOLE1:OLE1-RFP 688 G &…

   Promoter

   Available since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAG-miniGFP2-P2A-FusionRed

   Plasmid

   #188734

   Purpose

   Express miniGFP2 and FusionRed in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

   Insert

   miniGFP2
   

   Use

   AAV

   Tags

   FusionRed

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-M13-TEV-C-P2A-TdTomato

   Plasmid

   #92391

   Purpose

   produces AAV expressing Cal-light M13-TevC component and TdTomato

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

   Insert

   M13-TEV-C-P2A-TdTomato
   

   Use

   AAV and Synthetic Biology

   Tags

   TdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBT372

   Plasmid

   #125613

   Purpose

   Expresses evoCDA1-BE4max-NG in mammalian cells

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   evoCDA1-BE4max-NG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   F23S A123V I195F

   Promoter

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEMS-II-PEST

    Plasmid

    #204766

    Purpose

    GEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)

    Depositor

    Kate Meyer

    Article

    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    EGFP-PEST-m6Alinker-DHFR
    

    Use

    Tags

    Expression

    Mutation

    mutated all RACs in EGFP to RGC/RCC

    Promoter

    CMV

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. ABE8r-V106W

    Plasmid

    #208295

    Purpose

    Expresses ABE8r in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA8r-V106W-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106W, …

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. TurboID-V5_pRS415

    Plasmid

    #107167

    Purpose

    expresses V5-tagged TurboID in the yeast cytosol

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTlpA_mCherry_YafQ/DinJ

    Plasmid

    #229148

    Purpose

    Toxin-Antitoxin (Dinj/YafQ)

    Depositor

    Shrikrishnan Sankaran

    Article

    Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

    Insert

    mCherry, DinJ/YafQ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PtlpA (mCherry), DinJ/YafQ (Native)

    Available since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSIMcpf1

    Plasmid

    #153034

    Purpose

    For CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    Ascpf1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-GfaABC1D-GRAB_ATP1.0

    Plasmid

    #167579

    Purpose

    Express the ATP sensor GRAB_ATP1.0 in astrocytes

    Depositor

    Yulong Li

    Article

    Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

    Insert

    ATP sensor GRAB_ATP1.0 (P2RY1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    GfaABC1D

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFP-Tubulin-6

    Plasmid

    #56450

    Purpose

    Localization: Microtubules, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Tubulin (TUBA1B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pJIT163-eAFID-3

    Plasmid

    #167360

    Purpose

    For inducing predictable deletions in protoplasts and wheat plants

    Depositor

    Caixia Gao

    Article

    Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

    Insert

    A3Bctd-Cas9-UDG-T2A-AP lyase
    

    Use

    Tags

    Expression

    Plant

    Mutation

    WT

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. ND4.2-Right DdCBE-G1397-C- T1413I

    Plasmid

    #179685

    Purpose

    Expression of base editor in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_SOD2 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T1413I

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTF-lacZ-rfp

    Plasmid

    #153037

    Purpose

    CRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    CRISPR array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJL1-CRM197-4xDQNAT

    Plasmid

    #128395

    Purpose

    In vitro expression of CRM197 with C terminal 4xDQNAT glycosylation sites

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Diphtheria toxin
    

    Use

    Tags

    4xDQNAT glycosylation tag and 6xHis tag

    Expression

    Bacterial

    Mutation

    Inactivating G52E mutation

    Promoter

    T7

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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