We narrowed to 171,340 results for: addgene
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Plasmid#235314PurposeLentiviral expression of mRuby2 with 3' microRNA target site only (closed-loop)DepositorInsertmRuby2
UseLentiviral and Synthetic BiologyExpressionMammalianPromoterTRE3G (3rd-generation Tet system)Available SinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiX1-[TRE3G-TS.FF4x1-mRuby2(miRE.FF4)-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE
Plasmid#235322PurposeLentiviral expression of ComMAND closed-loop circuit regulating mRuby2, design 2DepositorInsertmRuby2
UseLentiviral and Synthetic BiologyExpressionMammalianPromoterTRE3G (3rd-generation Tet system)Available SinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiX1-[TRE3G-TS.FF4x1-mRuby2-miRE.FF4-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE
Plasmid#235324PurposeLentiviral expression of ComMAND closed-loop circuit regulating mRuby2, design 3DepositorInsertmRuby2
UseLentiviral and Synthetic BiologyExpressionMammalianPromoterTRE3G (3rd-generation Tet system)Available SinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 2xHA HCAR3
Plasmid#226811PurposeTet-inducible lentiviral vector for 2xHA HCAR3 expressionDepositorInsertHCAR3 (HCAR3 Human)
UseLentiviralTags2xHA tag followed by a GDPPVAT linkerMutationSequence optimized from NP_006009.2Available SinceMay 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-CKAP4(2xlumen)-WPRE-UbC-Emerald
Plasmid#225956PurposeLentiviral vector plasmid expressing human CKAP4 mutant with an additional luminal domain under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralExpressionMammalianMutationFull-length CKAP4 with additional luminal domain …PromoterSFFV and UbCAvailable SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-myc-STIM1-WPRE-UbC-Emerald
Plasmid#225939PurposeLentiviral vector plasmid expressing human stromal interaction molecule 1 (STIM1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsmyc-tagExpressionMammalianPromoterSFFV and UbCAvailable SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-myc-STIM2-WPRE-UbC-Emerald
Plasmid#225940PurposeLentiviral vector plasmid expressing human stromal interaction molecule 2 (STIM2) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsmyc-tagExpressionMammalianPromoterSFFV and UbCAvailable SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-myc-ATL1-WPRE-UbC-Emerald
Plasmid#225944PurposeLentiviral vector plasmid expressing human atlastin GTPase 1 (ATL1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsmyc-tagExpressionMammalianPromoterSFFV and UbCAvailable SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
CamKII-mtPyronicSF
Plasmid#228906PurposeExpression of mtPyronicSF in neurons targeted to the mitochondria serving as an optical sensor for mitochondrial pyruvate uptake. Backbone from Addgene plasmid 100834DepositorInsertpyronic SF
UseAAVTagsCox8 targettingExpressionMammalianPromoterCamKIIAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-Sun1-GFP-WPRE-BC12-pA
Plasmid#220764PurposeExpression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracingDepositorInsertSun1-GFP-WPRE-BC12
UseAAVPromoterCAGAvailable SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A 2xHA GPR137
Plasmid#226809PurposeGateway entry vector for GPR137DepositorInsertGPR137 (GPR137 Human)
UseGateway entryTags2xHA tag followed by a GDPPVAT linkerMutationSequence optimized from NP_001164351Available SinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
aTY50_pPGK1_Pcad_IRES_PuroR_WPRE
Plasmid#225348PurposeLentiviral vector for Pcad expressionDepositorAvailable SinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHC025 (∆CBBp)
Plasmid#226203PurposeElectroporation knockout plasmid (pK18sB) to delete the C. necator megaplasmid copy of the CBB operon (deltaCBBp).DepositorInsertHomology arms for megaplasmid copy of the CBB operonthe CBB operon (deltaCBBp).
UseSynthetic BiologyMutation∆cbbR ∆cbbLpSpXpYpEpFpPpTpZpGpKpApAvailable SinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCHC024 (∆SH)
Plasmid#226202PurposeElectroporation knockout plasmid (pK18sB) to delete the C. necator soluble hydrogenase operon (deltaSH).DepositorInsertHomology arms for soluble hydrogenase operon
UseSynthetic BiologyMutation∆hoxFUYHWI ∆hypA2B2F2Available SinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCHC023 (∆MBH)
Plasmid#226201PurposeElectroporation knockout plasmid (pK18sB) to delete the C. necator membrane-bound hydrogenase operon (deltaMBH).DepositorInsertHomology arms for membrane bound hydrogenase operon
UseSynthetic BiologyMutation∆hoxKGZMLOQRTV ∆hypA1B1F1CDEX ∆hoxABCJAvailable SinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-C-Solo-miRFP670nano3-H2BC11
Plasmid#227331PurposeDonor template for miRFP670nano3 insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)DepositorInsertH2BC11 Homology Arms flanking a miRFP670nano3 Tag (H2BC11 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-EFS-Puro-PLK4
Plasmid#227311PurposeDonor template for mStayGold-EFS-Puro insertion into the C-terminus of the PLK4 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px458-PLK4 (Addgene #227310)DepositorInsertPLK4 Homology Arms flanking a mStayGold-EFS-Puro Cassette (PLK4 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330-TP53
Plasmid#227318PurposeExpresses SpCas9 and a sgRNA targeting the N-terminus of TP53 for knock-in.DepositorAvailable SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-MAP4
Plasmid#227295PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of MAP4 for knock-in.DepositorAvailable SinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330-EZR
Plasmid#227293PurposeExpresses SpCas9 and a sgRNA targeting the C-terminus of EZR for knock-in.DepositorAvailable SinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-CEP192
Plasmid#227288PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of CEP192 for knock-in.DepositorInsertsgRNA Targeting C-terminus of CEP192 (CEP192 Human)
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
p40651-EFSNS-FMR1_e1-24xms2
Plasmid#222968PurposeMS2 mRNA reporter with intact FMR1 5' UTR and exon 1 sequenceDepositorInsertFMR1-31CGG 5' UTR and exon1
ExpressionMammalianAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG-sgRNA.PRDM1-ZEB2_CRISPRd
Plasmid#216171PurposeExpress the gRNA targeting the PRDM1-binding site in the human ZEB2 locus for the CRISPRd experimentDepositorAvailable SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-Sun1-GFP-WPRE-BC8-pA
Plasmid#220760PurposeExpression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracingDepositorInsertSun1-GFP-WPRE-BC8
UseAAVPromoterCAGAvailable SinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-Sun1-GFP-WPRE-BC10-pA
Plasmid#220762PurposeExpression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracingDepositorInsertSun1-GFP-WPRE-BC10
UseAAVPromoterCAGAvailable SinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-Sun1-GFP-WPRE-BC9-pA
Plasmid#220761PurposeExpression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracingDepositorInsertSun1-GFP-WPRE-BC9
UseAAVPromoterCAGAvailable SinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-Sun1-GFP-WPRE-BC11-pA
Plasmid#220763PurposeExpression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracingDepositorInsertSun1-GFP-WPRE-BC11
UseAAVPromoterCAGAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-eSpCas9-2A-GFP-sgRNA-Lig4
Plasmid#220494PurposeTo generate LIG4 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against LIG4 exon 3.DepositorAvailable SinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-Sun1-GFP-WPRE-BC7-pA
Plasmid#220759PurposeExpression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracingDepositorInsertSun1-GFP-WPRE-BC7
UseAAVPromoterCAGAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
p4A8_M59I_T94M_BC
Plasmid#217833Purpose4A8 variant in barcoded, yeast surface display plasmidDepositorInsert4A8 variant Fab
ExpressionYeastMutationVH: M59I, VL: T94MAvailable SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pInducer20-iCreb5(HA)
Plasmid#195023PurposeHA-tagged Creb5 in pInducer20 lentivirus destination vectorDepositorInsertHA-tagged Creb5 (full length, 508aa) (CREB5 Bovine)
UseLentiviralTags3xHAExpressionMammalianAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
GAL4-Creb5 (1- 128)
Plasmid#195031PurposeGAL4-Creb5 fusion construct (N-terminus of Creb5,128aa)DepositorInsertGAL4-Creb5 fusion construct (N-terminus of Creb5,128aa) (CREB5 Bovine)
ExpressionMammalianAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Neo-TNNT2-Blast
Plasmid#214014PurposeExpression of cardiac troponin TDepositorInsertTNNT2 (TNNT2 Human)
ExpressionMammalianAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Neo-TNNT2-Hygro
Plasmid#214015PurposeExpression of cardiac troponin TDepositorInsertTNNT2 (TNNT2 Human)
ExpressionMammalianAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Blast-TNNT2-Zeo
Plasmid#214018PurposeExpression of cardiac troponin TDepositorInsertTNNT2 (TNNT2 Human)
ExpressionMammalianAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Parkin(C431N)-A92mKO2
Plasmid#213545PurposeTransient mammalian expresssion of ligase-dead C431N Parkin, internally tagged with mKO2DepositorAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLv-EF1a-Parkin(R104A)-A92mKO2
Plasmid#213556PurposeLentiviral vector for stable expression of R104A Parkin, internally tagged with mKO2DepositorInsertParkin (PRKN Human)
UseLentiviralExpressionMammalianMutationR104A mutation in ParkinPromoterEF1aAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLv-CMV(trunc)-Parkin-A92mKO2
Plasmid#213549PurposeLentiviral vector for stable low expression of Parkin, internally tagged with mKO2DepositorAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLv-EF1a-Parkin(N273K)-A92mKO2
Plasmid#213555PurposeLentiviral vector for stable expression of N273K Parkin, internally tagged with mKO2DepositorInsertParkin (PRKN Human)
UseLentiviralExpressionMammalianMutationN273K mutation in ParkinPromoterEF1aAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLv-EF1a-Parkin(W403A)-A92mKO2
Plasmid#213558PurposeLentiviral vector for stable expression of W403A Parkin, internally tagged with mKO2DepositorInsertParkin (PRKN Human)
UseLentiviralExpressionMammalianMutationW403A mutation in ParkinPromoterEF1aAvailable SinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only