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pLKO.1-blast-cfRab8b_3

Plasmid

#26709

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rab8b RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Rab8b_3 RNAi.

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pAAVS1-NEO-CAG-BFP-pA-GFP-pA

Plasmid

#149344

Purpose

Vector for targeting the Replace reporter to the AAVS1 locus

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

BFP

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pYC25

Plasmid

#63914

Purpose

Recipient overexpressing vector to construct YFP tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

YFP

Expression

Yeast

Promoter

TEF1 (Saccharomyces cerevisiae)

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pYC66

Plasmid

#63929

Purpose

Recipient replicative vector to construct MYC tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

MYC

Expression

Yeast

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pML3

Plasmid

#108341

Purpose

MIDAS Level-3 destination vector. Used for initiating Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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Ube2i

Plasmid

#31420

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBE2I Human)

Tags

HA

Expression

Yeast

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pLKO.1-blast-cfRab25_4

Plasmid

#26712

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rab25 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Canine Rab25 RNAi.

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pYC16

Plasmid

#63913

Purpose

Recipient overexpressing vector to construct CFP tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

CFP

Expression

Yeast

Promoter

TEF1 (Saccharomyces cerevisiae)

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-cfRabin8_5

Plasmid

#26714

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rabin8 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Rabin8 alpha isoform RNAi

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pLKO.1-blast-cfRab25_2

Plasmid

#26711

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rab25 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

RNAi to canine Rab25.

Available Since

Dec. 14, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA5_TO_FRT_myc_GLUT4_2Ala_APEX2

Plasmid

#250767

Purpose

Mammalian cell expression of GLUT4 in which L500 and L503 are mutated to alanines

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

GLUT4(2Ala)

Tags

APEX2 and myc (in exofacial loop)

Expression

Mammalian

Mutation

L500 and L503 mutated to alanines

Promoter

CMV

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti_H1_VPS35_shRNA_UBC_SSF_SNAP_MOR_WT_P2A_GFP

Plasmid

#250765

Purpose

Mammalian cell expression of an shRNA against VPS35 and the mu opioid receptor

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

VPS35 shRNA

Tags

SSF-SNAP(for MOR)

Expression

Mammalian

Promoter

H1 for shRNA, UBC for MOR

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti_H1_Scramble_shRNA_UBC_SSF_SNAP_MOR_WT_P2A_GFP

Plasmid

#250764

Purpose

Mammalian cell expression of a scrambled shRNA and the mu opioid receptor

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

Scrambled VPS35 shRNA

Tags

SSF-SNAP (for MOR)

Expression

Mammalian

Promoter

H1 for shRNA, UBC for MOR

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti_SSF_MOR_2Ala_APEX2

Plasmid

#250774

Purpose

Mammalian cell expression of the mu opioid receptor with L387 and L390 mutated to alanines

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

MOR(2Ala)

Tags

APEX2 and Signal Sequence FLAG

Expression

Mammalian

Mutation

L387 and L400 are mutated to alanines

Promoter

UBC

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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GFP-RavZ C258A

Plasmid

#250879

Purpose

Transient mammalian expression of GFP-RavZ C258A

Depositor

Thomas Melia

Article

Fuller et al eLife 2025 14:RP107316

Insert

RavZ (ravZ Legionella pneumophila)

Expression

Mammalian

Mutation

C258A

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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GFP-RavZ

Plasmid

#250878

Purpose

Transient mammalian expression of GFP-RavZ

Depositor

Thomas Melia

Article

Fuller et al eLife 2025 14:RP107316

Insert

RavZ (ravZ Legionella pneumophila)

Expression

Mammalian

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK125

Plasmid

#245147

Purpose

pJAK081 carrying Pcwp2-mScarlet-I

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Insert

Pcwp2-mScarlet-I

Use

Bacterial knock-in

Promoter

Pcwp2

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK201

Plasmid

#245149

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 1 (AAGTCCTCG)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAK202

Plasmid

#245150

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 2 (TCTTGACCG)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAK203

Plasmid

#245151

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 3 (AACAACACC)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge