We narrowed to 354 results for: Rab1

1. RAB11A-BFP HDRT Source (pTR 145)

   Plasmid

   #112014

   Purpose

   DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with BFP

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   RAB11A-BFP HDRT (RAB11A Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET 15b+His SUMO Rab12 (Q101L)

   Plasmid

   #208371

   Purpose

   Bacterial expression of His-SUMO-tagged human Rab12 (Q101L)

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   His SUMO Rab12 (Q101L) (RAB12 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q101L

   Promoter

   Available since

   Dec. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-sgRab18.N-Cas9-T2A-mCherry-P2A-Puro

   Plasmid

   #129418

   Purpose

   Encoding Cas9 and sgRAB18.N for CRISPR/Cas9 mediated HDR tagging of endogenous human RAB18 N-terminus

   Depositor

   Elina Ikonen

   Article

   Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

   Insert

   SpCas9 and sgRAB18.N (RAB18 Human)
   

   Use

   CRISPR

   Tags

   T2A-mCherry-P2A-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiTet-RAB10-T2A-RABIF-P2A-mCherry BlastR

   Plasmid

   #186740

   Purpose

   Dox inducible RAB10-T2A-RABIF-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

   Depositor

   Richard Sherwood

   Article

   Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

   Insert

   RAB10, RABIF (RAB10, RABIF Human)
   

   Use

   Lentiviral

   Tags

   RAB10-T2A-RABIF-P2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-SFFV-RAB10 Q68L-WPRE-UbC-Emerald

   Plasmid

   #203803

   Purpose

   Lentiviral vector plasmid expressing human RAB10 mutation Q68L (constitutively active mutant) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis

   Inserts

   RAB10 (RAB10 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Q68L

   Promoter

   Available since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-SFFV-RAB10 T23N-WPRE-UbC-Emerald

   Plasmid

   #203802

   Purpose

   Lentiviral vector plasmid expressing human RAB10 mutation T23N (dominant negative mutant) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis

   Inserts

   RAB10 (RAB10 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   T23N

   Promoter

   Available since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLQ8069 - ACTB GFP-P2A + RAB11A mCherry-P2A CLIP Donor

   Plasmid

   #202689

   Purpose

   CLIP donor template to generate 2 knock-ins: GFP into ACTB, and mCherry in RAB11A.

   Depositor

   Stanley Qi

   Article

   Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

   Inserts

   CopGFP
   mCherry
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11

   Plasmid

   #174644

   Purpose

   Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11
   

   Use

   Tags

   Venus-SSPB and iLID-mCherry

   Expression

   Mammalian

   Mutation

   mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…

   Promoter

   Chicken beta-actin

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

   Plasmid

   #174645

   Purpose

   Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)-P2A-ILID-mCherry-RAB11
   

   Use

   Tags

   SSPB(micro)-Venus and iLID-mCherry

   Expression

   Mammalian

   Mutation

   SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…

   Promoter

   Chicken beta-actin

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA5-FRT-TO-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11

    Plasmid

    #174646

    Purpose

    Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes. Compatible with Flp-in TREX system.

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…

    Promoter

    CMV (with TetOn)

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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