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pDV17_pCMV-MCP-ADAR2dd(E488Q)-NES

Plasmid

#200406

Purpose

Expresses a compact, engineered version of ADAR2 constitutively in mammalian cells

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

MCP-ADAR2dd(E488Q)-NES

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

E488Q (hyperactive)

Promoter

CMV

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-hyPE2

Plasmid

#177173

Purpose

Expresses hyPE2 in mammalian cells

Depositor

Hyongbum Kim

Article

Song et al Nat Commun. 2021 Sep 23;12(1):5617. doi:

Insert

hyperPE2

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ5135: pCAG-BFP-GuideArray

Plasmid

#210606

Purpose

Guide array for hyperdCas12a-based transcriptional activator with AAAT synSeparator

Depositor

Stanley Qi

Article

Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

Insert

pCAG-BFP-GuideArray

Use

CRISPR

Tags

Expression

Mutation

Promoter

CAG

Available since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pZG61

Plasmid

#202575

Purpose

RNA editing in plants. Plasmid contains 35S-dADARcd-LIC1-ccdB-LIC2-35S-hptII

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Use

Tags

Expression

Plant

Mutation

E488Q

Promoter

Available since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-FOLD6

Plasmid

#186547

Purpose

FOLD6 "hyperfolder" green fluorescent protein: bacterial expression plasmid

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

FOLD6

Use

Tags

6xHis

Expression

Bacterial

Mutation

mGreenLantern-C48S/V68L/C70V/K101E/T105Y/S147P/K1…

Promoter

araB

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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MTK1_008

Plasmid

#123671

Purpose

Encodes ConLS and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector LS-2xHS4

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pZG71

Plasmid

#202577

Purpose

RNA editing in plants. Plasmid contains 35S-dADARcd-LIC1-ccdB-LIC2-dADARcd-35S-hptII

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Use

Tags

Expression

Plant

Mutation

E488Q

Promoter

Available since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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MTK1_009

Plasmid

#123672

Purpose

Encodes ConL1 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L1-2xHS4

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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MTK5_008

Plasmid

#123852

Purpose

Encodes ConRE' and the chicken hypersensitivity site 4 insulator as a Type 5 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector RE'-2xHS4

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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BC-A1-013

Plasmid

#78701

Purpose

A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a strong RBS.

Depositor

Brian Chow

Article

Magaraci et al ACS Synth Biol. 2016 Apr 25.

Insert

BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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BC-A1-014

Plasmid

#78702

Purpose

A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a strong RBS.

Depositor

Brian Chow

Article

Magaraci et al ACS Synth Biol. 2016 Apr 25.

Insert

BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP-Terminator

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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MTK1_010

Plasmid

#123673

Purpose

Encodes ConL2 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L2-2xHS4

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_011

Plasmid

#123674

Purpose

Encodes ConL3 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L3-2xHS4

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
BC-A1-011

Plasmid

#78699

Purpose

A strong promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a weak RBS.

Depositor

Brian Chow

Article

Magaraci et al ACS Synth Biol. 2016 Apr 25.

Insert

BBa_J23100-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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BC-A1-009

Plasmid

#78696

Purpose

A strong promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a strong RBS.

Depositor

Brian Chow

Article

Magaraci et al ACS Synth Biol. 2016 Apr 25.

Insert

BBa_J23100-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pGG-PIP61

Plasmid

#196799

Purpose

Contains HRM1 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

HYPERSENSITIVE RESPONSE MARKER 1 (AT5G17760 Mustard Weed)

Use

Greengate (gg) entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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OSCA1.2-K435L-peGFP-N1

Plasmid

#228586

Purpose

Expresses OSCA1.2-K435L-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

hyperosmolality-gated Ca2+ permeable channel (OSCA) 1.2 (AT4G22120 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

K435L

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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OSCA1.2-peGFP-N1

Plasmid

#228585

Purpose

Expresses OSCA1.2-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

hyperosmolality-gated Ca2+ permeable channel (OSCA) 1.2 (AT4G22120 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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OSCA1.2-A439K-peGFP-N1

Plasmid

#228589

Purpose

Expresses OSCA1.2-A439K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

hyperosmolality-gated Ca2+ permeable channel (OSCA) 1.2 (AT4G22120 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

A439K

Promoter

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-hfYFP-TEV-Bcx

Plasmid

#191195

Purpose

Fusion of hfYFP and Bacillus circulans xylanase separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

hfYFP & Bcx fusion

Use

Tags

6xHis and Bacillus circulans xylanase

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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