Addgene

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ZE45 Dvl-2 pCS2P+

Plasmid

#17086

Depositor

Randall Moon

Article

Waxman et al Development. 2004 Dec. 131(23):5909-21.

Insert

Dvl-2 (dvl2 Zebrafish)

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pHis-zfCSF3a

Plasmid

#65612

Purpose

express soluble His-tagged zebrafish CSF3a/Gcsfa in E. coli

Depositor

Petr Bartunek

Article

Stachura et al Blood. 2011 Aug 4;118(5):1274-82. doi: 1

Insert

Csf3a (csf3a Zebrafish)

Use

Tags

(His)6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only
ZE33 Prickle-1-GFPnopren-pCS2+

Plasmid

#17074

Depositor

Randall Moon

Article

Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

Insert

Prickle-1 (prickle1a Zebrafish)

Use

Zebrafish expression

Tags

GFP

Expression

Mutation

Pk1GFPnopren deletes the C-terminal six amino aci…

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pCS2-SauCas9

Plasmid

#174040

Purpose

In vitro transcription of SauCas9 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

SauCas9

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pT3TS-HiFi-nCas9n-A72

Plasmid

#125646

Purpose

micro-injection of poly-adenylated HiFi-nCas9n RNA

Depositor

Jason Berman

Article

Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

Insert

HiFi-nCas9n

Use

Rna in vitro transcription vector

Tags

Expression

Mutation

R691A from wild-type Cas9

Promoter

T3

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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ZE41 Dapper-1 pCS2P+

Plasmid

#17082

Depositor

Randall Moon

Article

Waxman et al Development. 2004 Dec. 131(23):5909-21.

Insert

Dapper-1 (dact1 Zebrafish)

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pCx55.5-TrpR

Plasmid

#86373

Purpose

for zebrafish transgenesis, expression of TrpR from Cx55.5 promoter, also expresses mCherry

Depositor

Rachel Wong

Article

Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

Insert

TrpR

Use

Zebrafish

Tags

Expression

Mutation

Promoter

cx55.5

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-kcnn1b

Plasmid

#164957

Purpose

Zebrafish kcnn1b gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnn1b (kcnn1b Zebrafish)

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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B3rec.zf1

Plasmid

#108354

Purpose

Zebrafish codon optimized B3 recombinase that acts on blown-out sites

Depositor

Harold Burgess

Article

Tabor et al Curr Biol. 2018 Aug 20;28(16):2527-2535.

Insert

B3 recombinase

Use

Synthetic Biology

Tags

Expression

Mutation

Zebrafish codon optimized

Promoter

lac

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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ZE42 myc-Dapper-1 pCS2P+

Plasmid

#17083

Depositor

Randall Moon

Article

Waxman et al Development. 2004 Dec. 131(23):5909-21.

Insert

Dapper-1 (dact1 Zebrafish)

Use

Zebrafish expression

Tags

myc

Expression

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pDEST-CMV-EGFPCAAX

Plasmid

#232140

Purpose

Expresses EGFP with a CAAX motif under control of a CMV promoter

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

EGFPCAAX

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

CMV

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCRII-mogat3b in situ probe

Plasmid

#232136

Purpose

zebrafish mogat3b anti-sense in situ probe, linearize with BamHI, T7 polymerase

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

mogat3b

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

SP6/T7

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pcRII-mogat3a in situ probe

Plasmid

#232135

Purpose

zebrafish mogat3a anti-sense in situ probe, linearize with NotI, SP6 polymerase

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

mogat3a

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

SP6/T7

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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mhc2dab:mCherry

Plasmid

#68997

Purpose

for making transgenic fish

Depositor

David Traver

Article

Wittamer et al Blood. 2011 Jun 30;117(26):7126-35. doi:

Insert

mhc2dab (mhc2dab Zebrafish)

Use

Zebrafish expression

Tags

mcherry

Expression

Mutation

Promoter

mhc2dab

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-Dnase1l1l-mRFP

Plasmid

#169002

Purpose

Express zebrafish Dnase1l1l-mRFP in zebrafish

Depositor

Noboru Mizushima

Article

Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

Insert

Dnase1l1l-mRFP (dnase1l3l Zebrafish)

Use

Unspecified

Tags

mRFP

Expression

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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nm-mhc-b

Plasmid

#78709

Purpose

Encodes zebrafish nm-mhc-b

Depositor

Sarah Childs

Article

Georgijevic et al Dev Dyn. 2007 Jun;236(6):1623-32.

Insert

nm-mhc-b (myh10 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only
pCRII HSPB7

Plasmid

#61034

Purpose

Probe for HSPB7 for in situ hybridization in zebrafish

Depositor

Lara Hutson

Article

Marvin et al Dev Dyn. 2008 Feb;237(2):454-63.

Insert

HSPB7 (hspb7 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
pTol2CG2-ubi:QFGal4-SV40pA

Plasmid

#155119

Purpose

Tol2 vector for ubiquitous expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

ubi:QFGal4 (ubb Budding Yeast, Zebrafish, Neurospora crassa)

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

ubiquitin B

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pT22gcfUbm5En25EE4PCh(#552)

Plasmid

#184092

Purpose

cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Amblard et al Commun Biol. 2020 Sep 29;3(1):536. doi:

Insert

5myc-chkEn2(5E)-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

5xMyc

Expression

Mutation

chkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT22Ubm4Eng2bKRPC(#493)

Plasmid

#184091

Purpose

cyclofen-inducible fish Eng2b (mut) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Amblard et al Commun Biol. 2020 Sep 29;3(1):536. doi:

Insert

4myc-ZfEng2b(WW>KK)-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

4xMyc

Expression

Mutation

ZfEng2b: W141K, W144K; ERT2: G400V, M543A, L544A,…

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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