We narrowed to 8,771 results for: ef1;

1. pSmart-EF1alpha-AcrIC9-NLS

   Plasmid

   #207397

   Purpose

   Expresses AcrIC9 with NLS in mammalian cells

   Depositor

   Yan Zhang

   Article

   Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

   Insert

   EF1a-AcrIC9-bGH polyA
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pOttc556 - pAAV EF1a iCre

   Plasmid

   #210416

   Purpose

   AAV viral vector packaging plasmid that expresses "improved Cre recombinase"

   Depositor

   Christopher Richie, Brandon Harvey

   Insert

   iCre
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti7-3_EF1ap-mRuby2_hU6p_sgSTK4i_#1 (BlasR)

   Plasmid

   #209776

   Purpose

   CRISPRi for STK4

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   Nluc, sgSTK4i, mRuby2 (STK4 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPV-EF1α-mRxxFP1(DMDex55_10)-iP-A

   Plasmid

   #204622

   Purpose

   Single-strand annealing (SSA) vector for monitoring crRNA(DMDex55)'s activity. DMD intron 55 region (downstream of exon 55). Puromycin selectable.

   Depositor

   Akitsu Hotta

   Article

   Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

   Insert

   mRFP1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPV-C1-crRNA-cloning-EF1a-BA

   Plasmid

   #204623

   Purpose

   piggyBac vector for crRNA cloning and expression. Blasticidin selectable.

   Depositor

   Akitsu Hotta

   Article

   Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLV-REPORT(EF1a no GFP)

   Plasmid

   #172339

   Purpose

   mnucTomato

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   monomeric nuclear Tomato
   HygR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3x SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLV-REPORT(EF1a)TetR

   Plasmid

   #172352

   Purpose

   Tet Repressor reporter

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   TetR
   d2eGFP
   HygR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3x SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV/EF1a::DIO-iLMO2

   Plasmid

   #129482

   Purpose

   AAV2 transfer plasmid for Cre-dependent expression of iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical

   Depositor

   Robert Gross

   Article

   Jaiswal et al J Neurosci Res. 2017 Aug 3. doi: 10.1002

   Insert

   iLMO2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Ef1a-DIO-Aion-citrine

   Plasmid

   #192571

   Purpose

   AAV-vector for optogenetic long-term silencing of Cre-positive neurons

   Depositor

   Simon Wiegert

   Insert

   Aion
   

   Use

   AAV

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

   Promoter

   Ef1-alpha

   Available since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Ef1a-DIO-somAion-citrine

    Plasmid

    #192573

    Purpose

    AAV-vector for optogenetic long-term silencing of Cre-positive neurons

    Depositor

    Simon Wiegert

    Insert

    somAion
    

    Use

    AAV

    Tags

    Citrine - soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

    Promoter

    Ef1-alpha

    Available since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKLV2-EF1a-GFP RanSen

    Plasmid

    #184697

    Purpose

    Lentiviral transfer plasmid for expression of GFP RanSen

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    GFP RanSen
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKLV2-EF1a-ArgSen (-)NLS

    Plasmid

    #184689

    Purpose

    Lentiviral transfer plasmid for expression of ArgSen (-)NLS

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    ArgSen (-)NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLV-EF1a-ArgSen (-)NLS

    Plasmid

    #184698

    Purpose

    Lentiviral transfer plasmid for expression of ArgSen (-)NLS

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    ArgSen (-)NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAID1.2-EF1a-NmClover3-hsLem2

    Plasmid

    #179524

    Purpose

    Express OsTIR1 and mClover3-mAID-hsLem2 in mammalian cells under the control of EF1a promoter

    Depositor

    Yasushi Hiraoka

    Article

    Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

    Insert

    LEMD2 (LEMD2 Human)
    

    Use

    Tags

    mClover3-mAID

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEF1α-mouse-CHMP3(147)-HA

    Plasmid

    #154177

    Purpose

    expresses mouse CHMP3(147) in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    CHMP3 (Chmp3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C-terminal truncation of CHMP3 at amino acid 147

    Promoter

    EF1alpha

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLV x EF1a_EKAREN4(TA)-ires-blast

    Plasmid

    #167831

    Purpose

    Control sensor for EKAREN4

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4(TA)
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPD1024 EF1alpha TetON3G in TU5

    Plasmid

    #139261

    Purpose

    Expresses TetON3G under hEF1alpha in TUPV5

    Depositor

    Joshua Leonard

    Insert

    TetON3G
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1alpha

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJM469 EF1alpha TetON3G in TU4

    Plasmid

    #139260

    Purpose

    Expresses TetON3G under hEF1alpha in TUPV4

    Depositor

    Joshua Leonard

    Insert

    TetON3G
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1alpha

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJM422 EF1alpha TetON3G in TU3

    Plasmid

    #139259

    Purpose

    Expresses TetON3G under hEF1alpha in TUPV3

    Depositor

    Joshua Leonard

    Insert

    TetON3G
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1alpha

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Clim EF1 MCH (CEM)

    Plasmid

    #104444

    Purpose

    Expresses mCherry under elongation factor 1 promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.

    Depositor

    Inaki Ruiz-Trillo, Elena Casacuberta

    Insert

    mCherry
    

    Use

    Expression in corallochytrium (single-celled euka…

    Tags

    Expression

    Mutation

    Promoter

    EF1

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRS416-PTEF1-MCP-3P-ECM33_intron

    Plasmid

    #127601

    Purpose

    low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 3P-ECM33 intron inserted into URA3

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    ECM33 intron with MS2 hairpins inserted near 3′ end of intron
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRS416-PTEF1-MCP-5M-ECM33_intron

    Plasmid

    #127600

    Purpose

    low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5M-ECM33 intron inserted into URA3

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    ECM33 intron with MS2 hairpins replacing sequence near 5′ end of intron
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRS416-PTEF1-MCP-5P-ECM33_intron

    Plasmid

    #127599

    Purpose

    low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5P-ECM33 intron inserted into URA3

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    ECM33 intron with MS2 hairpins inserted near 5′ end of intron
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRS416-PTEF1-MCP-ECM33_intron

    Plasmid

    #127598

    Purpose

    low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, ECM33 intron inserted into URA3

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    ECM33 intron
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRS416-PTEF1-MCP-no_intron

    Plasmid

    #127597

    Purpose

    low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    1xFLAG-MCP expression cassette
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSMPUW_6.18_Ex_Ef1a-HA-EGFP-SV40-Zeo

    Plasmid

    #128454

    Purpose

    lentiviral expression vector with Ef1a promoter, N-terminal HA tag, EGFP gene, SV40 promoter, and Zeo Resistance gene

    Depositor

    Lindy Barrett

    Insert

    Ef1a promoter, N-terminal HA tag, EGFP gene, SV40 promoter, and Zeocin Resistance gene
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. KA601: pMVP (L3-L2) pA; EF1a::TETa-pA

    Plasmid

    #121805

    Purpose

    pMVP L3-L2 entry plasmid, contains polyA + EF1a-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven TETa downstream of gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    polyA + EF1a::TETa-polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. IY704: pMVP (L1-R5) EF1a promoter

    Plasmid

    #121693

    Purpose

    pMVP L1-R5 entry plasmid, contains EF1a promoter for 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    EF1a promoter
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. EF1a-3NLS-Sox2TALE-GCN4

    Plasmid

    #120548

    Purpose

    Express 3xNLS-Sox2 TALE-GCN4 engineered to bind a site in the human SOX2 gene

    Depositor

    Yu Wang

    Article

    Zhao et al ACS Chem Biol. 2018 Mar 16;13(3):609-617

    Insert

    NLS, Sox2 TALE, GCN4 (SOX2 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. EF1a-3NLS-NGN2TALE-GCN4

    Plasmid

    #120547

    Purpose

    Express 3xNLS-NGN2 TALE-GCN4 engineered to bind a site in the human NGN2 ene

    Depositor

    Yu Wang

    Article

    Zhao et al ACS Chem Biol. 2018 Mar 16;13(3):609-617

    Insert

    NLS, NGN2 TALE, GCN4 (NEUROG2 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCDF1-R310_RASA2-EF1-Puro

    Plasmid

    #114097

    Purpose

    Express mutant RASA2 in mamalian cells

    Depositor

    Yardena Samuels

    Article

    Arafeh et al Nat Genet. 2015 Dec;47(12):1408-10. doi:

    Insert

    RASA2 (RASA2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    R310*

    Promoter

    CMV

    Available since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMSCV40-EF1a-hBCL10(1-114)-SO

    Plasmid

    #75257

    Purpose

    retroviral expression vector for expression of StrepOne-Tagged human BCL10 (AS 1-116)

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    Bcl10 (BCL10 Human)
    

    Use

    Retroviral

    Tags

    StrepOne Tag

    Expression

    Mammalian

    Mutation

    AS 1-116 (CARD-domain)

    Promoter

    pMSCV-LTRs, EF1a

    Available since

    June 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCDH-EF1-Fon-copGFP

    Plasmid

    #72260

    Purpose

    Express copGFP when FLP is expressed by another vector

    Depositor

    Kazuhiro Oka

    Insert

    copGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    elongation factor-1

    Available since

    Jan. 11, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pGEX-2TK-WW-EF1+EF2

    Plasmid

    #18080

    Depositor

    Marius Sudol

    Article

    Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

    Insert

    Dystrophin (DMD Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2008

    Availability

    Academic Institutions and Nonprofits only

35. pDSM-bc-Psmtef1-CAD-Tyhi9

    Plasmid

    #127730

    Purpose

    Yeast pathway position 3. CAD transcription unit with the S. mikatae TEF1 promoter and YHI9 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    cis-aconitate decarboxylase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Psmtef1

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDSM-cd-Ptef1-ACO-Tyol036w

    Plasmid

    #127733

    Purpose

    Yeast pathway position 4. ACO transcription unit with the TEF1 promoter and YOL036w terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    aconitase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Ptef1

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDSM-cd-Psptdh3-ACO-Tagtef1

    Plasmid

    #127734

    Purpose

    Yeast pathway position 4. ACO transcription unit with the S. paradoxus TDH3 promoter and A. gossypii TEF1 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    aconitase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Psptdh3

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDSM-ef-Ptef1-CSC-Tpdc1

    Plasmid

    #127739

    Purpose

    Yeast pathway position 6. CSC transcription unit with the TEF1 promoter and PDC1 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    citrate synthase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Pfba1

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. lenti-EF1a-dCas9-VPR-Puro

    Plasmid

    #99373

    Purpose

    3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    (Sp)dCas9-VPR-P2A-Puro
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. EF1a-dCas9-KRAB-GFP backbone

    Plasmid

    #194281

    Purpose

    Vector for CRISPRi-GFP expression ready for gateway cloning of sgRNA

    Depositor

    Elizabeth Kirby

    Article

    Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

    Insert

    dCas9-KRAB-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. U6-NT sgRNA; EF1a-dCas9-KRAB-GFP

    Plasmid

    #194284

    Purpose

    EF1a-dCas9-KRAB-GFP with nontarget sgRNA

    Depositor

    Elizabeth Kirby

    Article

    Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

    Insert

    dCas9-KRAB-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pT3-EF1A-MYC-IRES-luc

    Plasmid

    #129775

    Purpose

    Transposon based vector that expresses human MYC followed by IRES and firefly luciferase

    Depositor

    Amaia Lujambio

    Article

    Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82

    Insert

    human MYC (MYC Human)
    

    Use

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE

    Plasmid

    #198516

    Purpose

    Expresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Cre-dependent recombination.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Has Service

    AAV1 and AAV5

    Insert

    PdCO
    

    Use

    AAV and Cre/Lox

    Tags

    Rho1D4 and mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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44. pAAV-Ef1a-fDIO-eGFP-WPRE

    Plasmid

    #203843

    Purpose

    Flp-dependent EGFP expression

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCDH-EF1-rtTA-PGK-BSD

    Plasmid

    #192885

    Purpose

    expression of rtTA

    Depositor

    Kazuhiro Oka

    Insert

    rtTA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pCDH-EF1a-mCherry-EGFP-LC3B

    Plasmid

    #170446

    Purpose

    Lentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.

    Depositor

    Sang-Hun Lee

    Article

    Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

    Insert

    mCherry-GFP-LC3 (MAP1LC3B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pLenti EF1a GFP-mCherry-mPlin1

    Plasmid

    #189005

    Purpose

    Fluorescent reportor for lipophagy

    Depositor

    Atsushi Hoshino

    Article

    Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

    Insert

    GFP, mChery, Plin1 (Plin1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pAAV-EF1a-Con/Fon-GCaMP6M

    Plasmid

    #137119

    Purpose

    Intersectional viral expression of GCaMP6M in cells expressing both Cre AND Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-GCaMP6M
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    Removed RSET tag

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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49. plenti2-EF1a-ChR2-EYFP-WRPE

    Plasmid

    #125255

    Purpose

    3rd gen lentiviral expression of ChR2 with mutation fused to EYFP driven by EF1a promoter for optogenetic activation

    Depositor

    Roger D. Kamm

    Article

    Osaki et al Sci Adv. 2018 Oct 10;4(10):eaat5847. doi

    Insert

    hChR2
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. pLV-SV40-puro-EF1a-HA-NLGN1-bc

    Plasmid

    #200167

    Purpose

    Expresses HA-tagged human NLGN1

    Depositor

    Paul Blainey

    Article

    Le et al Cell Rep. 2025 Jun 24;44(7):115889. doi:

    Insert

    Neuroligin-1 (NLGN1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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