Addgene

We narrowed to 1,174 results for: Aspa

Showing: 421 - 440 of 1174 results

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pTYF-super-mfSERT-0.5k-Venus-WPRE

Plasmid

#156395

Purpose

Expresses Venus under SERT gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfvAChT-1.1k-Venus-WPRE

Plasmid

#156390

Purpose

Expresses Venus under vAChT gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfCCK-3.9k-Venus-WPRE

Plasmid

#156391

Purpose

Expresses Venus under CCK gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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HA-Casp-2L

Plasmid

#157932

Purpose

Over expression of Casp-2L

Depositor

Baoji Xu

Article

Xu et al Nat Commun. 2019 Aug 9;10(1):3622. doi:

Insert

Casp2 (Casp2 Mouse)

Use

Tags

HA and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pJK382

Plasmid

#71574

Purpose

Produces Thermoplasma acidophilum citrate synthase, Asp317>Asn mutant (TpCS-D317N)

Depositor

T. J. Kappock

Article

Kurz et al Biochemistry. 2009 Aug 25;48(33):7878-91

Insert

citrate synthase

Use

Tags

Expression

Bacterial

Mutation

changes aspartate-317 to asparagine; silent mutat…

Promoter

recA

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pET151-hUCH37 DM

Plasmid

#61931

Purpose

Expression of hUCH37 (ISF1) M269E/N291D in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

hUCH37 (ISF1) M269E/N291D (UCHL5 Human)

Use

Tags

6x HIS

Expression

Bacterial

Mutation

Changed methionine 269 to glutamate and asparagin…

Promoter

T7

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pClneoEGFPC-let60 K16N

Plasmid

#66861

Purpose

Express GFP-tagged dominat negative let60, in which Lysine 16 is mutated to asparagine, in mammalian cells

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

Insert

Let-60 (let-60 Nematode)

Use

Tags

GFP

Expression

Mammalian

Mutation

Lysine 16 is mutated to asparagine

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pFloxin-Ofd1-Myc-KDD359-361FSY

Plasmid

#24578

Depositor

Jeremy Reiter

Article

Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Insert

Ofd1 (Ofd1 Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

Myc

Expression

Mutation

Lysine-Aspartate-Aspartate 359-361 changed to Phe…

Promoter

Available since

May 27, 2010

Availability

Academic Institutions and Nonprofits only

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pCAG.CASP3-Myc

Plasmid

#200119

Purpose

Expresses C-terminal Myc-tagged caspase 3 in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

caspase 3 (CASP3 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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PMXS-SLC1A3

Plasmid

#72873

Purpose

The retroviral SLC1A3 vector was generated by cloning an human SLC1A3 PCR fragment (https://www.addgene.org/32813/)into the pMXS-ires-blast vector by Gibson Assembly

Depositor

David Sabatini

Article

Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

Insert

SLC1A3 (SLC1A3 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP/golgin-160

Plasmid

#180630

Purpose

Expression of WT golgin-160

Depositor

Carolyn Machamer

Article

Mancini et al J Cell Biol. 2000 May 1;149(3):603-12. d

Insert

golgin-160 (GOLGA3 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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2XMyc-LRRK2-3XKD

Plasmid

#25366

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Tags

2XMyc

Expression

Mammalian

Mutation

"Kinase Dead" Lysine 1906 mutated to A…

Promoter

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-mCasp8

Plasmid

#208782

Purpose

Inducibly expresses mouse caspase8 fused to light-activatable Cry2

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

mCherry mCaspase8 Cry2 (Casp8 Mouse)

Use

Lentiviral

Tags

Cry2 and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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FL MALT1 WT in pET29b-Strep

Plasmid

#48968

Purpose

Expresses Full length wild type MALT1 in e.coli

Depositor

Guy Salvesen

Article

Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

Insert

MALT1 isoform A (MALT1 Human)

Use

Tags

His and Strep

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only
pET26b-SET

Plasmid

#8822

Depositor

Judy Lieberman

Article

Beresford et al J Biol Chem 2001 Nov 16;276(46):43285-93

Insert

SET (SET Human)

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only
pTripz Casp1-IRES-mCherry

Plasmid

#187294

Purpose

This is Caspase1 from mouse (NM_009807.2) with its CARD domain replaced by the FKBP-V domain (dimerising domain).

Depositor

Alpha Yap

Article

Bonfim-Melo et al Cell Rep. 2022 Feb 1;38(5):110316. doi:

Insert

Caspase1 (Casp1 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Promoter

CMV

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCS6-StaPLd-Casp9

Plasmid

#111511

Purpose

Expresses a tandem caspase-9 dimer, governed by a StaPL(AI) module, in mammalian cells. [AI = asunaprevir inhibited].

Depositor

Michael Lin

Article

Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi

Insert

StaPLd-Casp9

Use

Tags

Expression

Mammalian

Mutation

The HCV NS3 protease carries V36M, T54A, and S122…

Promoter

CMV

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pTU387

Plasmid

#178981

Purpose

pACYC Duet-1 containing full Type III-E system Candidatus “Scalindua brodae” CRISPR array

Depositor

Stan Brouns

Article

van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

Insert

CRISPR array from Candidatus Scalindua brodae

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pTU425

Plasmid

#178984

Purpose

pACYC containing Candidatus “Scalindua brodae” TPR-CHAT H585A C627A (codon-optimized)

Depositor

Stan Brouns

Article

van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

Insert

TPR-CHAT H585A C627A

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag4A-MITF-M (D380N)

Plasmid

#31248

Depositor

Yardena Samuels

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

MITF-M (MITF Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Aspartic acid 380 changed to Asparagine

Promoter

Available since

July 22, 2011

Availability

Academic Institutions and Nonprofits only