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Plasmid#40076DepositorTypeEmpty backboneUseTagsDendra2 PAFP, S-peptide, and TEV protease cleavag…ExpressionWormMutationPromoterpie-1Available sinceAug. 24, 2012AvailabilityAcademic Institutions and Nonprofits only
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pEPV-DsRed2-ER-5
Plasmid#170571PurposeExpresses DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide after signal peptide cleavage.DepositorInsertEPV-DsRed2-ER-5
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSM03
Plasmid#139043PurposeMosSCI SapTrap assembly: Cassette 3 for 4-cassette systemDepositorInsertdendra2 (includes STOP codon)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pSM12
Plasmid#139035PurposeMosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette systemDepositorInsertdendra2 (no STOP codon)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLCS105
Plasmid#209358PurposeGateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal Dendra2 fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAPV-DsRed2-ER-5
Plasmid#170570PurposeExpresses DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after signal peptide cleavage.DepositorInsertAPV-DsRed2-ER-5
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pAPV-DsRed2-FLAG-ER-5
Plasmid#170622PurposeExpresses DsRed2-FLAG targeted to the ER lumen with an N-terminal APV tripeptide after signal peptide cleavage. The protein is efficiently retrieved due to exposure of KDEL.DepositorInsertAPV-DsRed2-ER-5
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJRA105
Plasmid#209338PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal Dendra2 fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAPV-FLAG-DsRed2-ER-5
Plasmid#170620PurposeExpresses FLAG-DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after SP cleavage.DepositorInsertAPV-FLAG-DsRed2-ER-5
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPV-FLAG-DsRed2-ER-5
Plasmid#170621PurposeExpresses FLAG-DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide after signal peptide cleavage.DepositorInsertEPV-FLAG-DsRed2-ER-5
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAPV-mEmerald-DsRed2-ER-5
Plasmid#170681PurposeExpresses mEmerald-DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV and poorly exposed KDEL.DepositorInsertAPV-mEmerald-DsRed2-ER-5
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAPV-DsRed2-mEmerald-ER-5
Plasmid#170715PurposeExpresses DsRed2-mEmerald targeted to the ER lumen. The encoded protein is effectively retrieved back to the ER due to exposed KDEL despite the N-terminal APV.DepositorInsertAPV-DsRed2-mEmerald-ER-5
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
TPC2-G-GECO1.2
Plasmid#207141PurposeFusion of Ca2+ reporter and lysosomal Ca2+-permeable channelDepositorInsertTPC2N2 (TPCN2 Human)
UseTagsG-GECO1.2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
TPC1-G-GECO1.2
Plasmid#207143PurposeFusion of Ca2+ reporter and endosomal Ca2+-permeable channelDepositorInsertTPCN1 (TPCN1 Human)
UseTagsG-GECO1.2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEG584
Plasmid#40191DepositorInsertMex-5 (RNAi resistant) (mex-5 Nematode)
UseTagsDendra2 PAFP (sequence optimized for worm express…ExpressionWormMutationExon 2 recoded to be RNAi-resistantPromoter4.4 kb mex-5 promoter fragmentAvailable sinceSept. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEG587
Plasmid#40196DepositorInsertMex-5 (S458A) (RNAi resistant) (mex-5 Nematode)
UseTagsDendra2 PAFP (sequence optimized for worm express…ExpressionWormMutationS458A; exon 2 recoded to be RNAi-resistantPromoter4.4 kb mex-5 promoter fragmentAvailable sinceSept. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEG585
Plasmid#40192DepositorInsertMex-5 (S404A) (RNAi resistant) (mex-5 Nematode)
UseTagsDendra2 PAFP (sequence optimized for worm express…ExpressionWormMutationS404A; exon 2 recoded to be RNAi-resistantPromoter4.4 kb mex-5 promoter fragmentAvailable sinceSept. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEG634
Plasmid#40121DepositorInsertMex-5 (aa244-468) (mex-5 Nematode)
UseGateway destination vectorTagsDendra2 PAFP (sequence optimized for worm express…ExpressionWormMutationcontains aa244-468Promoter4.4 kb mex-5 promoter fragmentAvailable sinceSept. 19, 2012AvailabilityAcademic Institutions and Nonprofits only