We narrowed to 2,324 results for: PUC19

1. pdadhDN-tefAO217

   Plasmid

   #227341

   Purpose

   Replacement of the adh gene with the expression cassette of the AO217 gene

   Depositor

   Jun-ichi Maruyama

   Insert

   Flanking regions of adh (AO090009000634) and expression cassette of AO217 (AO090120000217)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ppG-ple12

   Plasmid

   #227339

   Purpose

   Heterologous production of pleuromutilin in Aspergillus oryzae

   Depositor

   Jun-ichi Maruyama

   Insert

   The upstream/downstream regions of the pyrG gene and expression cassettes of the ple1 and ple2 genes
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pwA-ple34

   Plasmid

   #227337

   Purpose

   Heterologous production of pleuromutilin in Aspergillus oryzae

   Depositor

   Jun-ichi Maruyama

   Insert

   The upstream/inside regions of the wA gene and expression cassettes of the ple3 and ple4 genes
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSLQ5429_pUC_hU6-crScaffold_EF1a-BFP

   Plasmid

   #155306

   Purpose

   Rfx Cas13d guide cloning pUC19 vector with constitutively expressed tagBFP

   Depositor

   Stanley Qi

   Article

   Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

   Insert

   Rfx Cas13d CRISPR RNA BFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, EF1a

   Available since

   July 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTOX-donor

   Plasmid

   #132516

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   TOX (TOX Human)
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   none

   Promoter

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTBX18-donor

   Plasmid

   #176370

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Kevin White, Barbara Stranger

   Insert

   TBX18 (TBX18 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pZNF468-donor

   Plasmid

   #176350

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Kevin White, Barbara Stranger

   Insert

   ZNF468 (ZNF468 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUC-dirTopo-P2A-aGFP

   Plasmid

   #61974

   Purpose

   A directional cloning vector of PCR-amplified cDNAs for mammalian expression

   Depositor

   Hiroshi Udo

   Inserts

   lacZ alpha
   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   It contains silent mutations

   Promoter

   Available since

   Feb. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUC-dirTopo-AU1-aGFP

   Plasmid

   #61973

   Purpose

   A directional cloning vector of PCR-amplified cDNAs for mammalian expression

   Depositor

   Hiroshi Udo

   Inserts

   lacZ alpha
   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   It contains silent mutations

   Promoter

   Available since

   Feb. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIAeBlue

    Plasmid

    #185891

    Purpose

    In vivo gene amplification (HamAmp) of AeBlue expression module at RPL25 locus

    Depositor

    Claudia Vickers

    Insert

    PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>AeBlue>TPGK1- PBTS1> RPL25(partial; Arm2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAW8-aid-Turg1:kanMX6

    Plasmid

    #169354

    Purpose

    C-terminal aid degron cassette with kanMX6 marker for S. pombe

    Depositor

    Tony Carr

    Insert

    auxin-inducible degron (aid)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pOTX2-donor

    Plasmid

    #176358

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White, Barbara Stranger

    Insert

    OTX2 (OTX2 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIEforRed

    Plasmid

    #185892

    Purpose

    In vivo gene amplification (HamAmp) of EforRed expression module at RPL25 locus

    Depositor

    Claudia Vickers

    Insert

    PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>EforRed>TPGK1-PBTS1> RPL25(partial; Arm2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSTAT5B-donor

    Plasmid

    #176339

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White, Barbara Stranger

    Insert

    STAT5B (STAT5B Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMDW133

    Plasmid

    #227248

    Purpose

    Lambda DNA for torsional templates

    Depositor

    Michelle Wang

    Insert

    Lambda DNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pXylP-HygR

    Plasmid

    #168131

    Purpose

    Expression of HygR from the xylose-inducible xylP promoter

    Depositor

    Michael Bromley

    Insert

    XylP-HygR
    

    Use

    Aspergillus expression

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    xylP

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOSR1-donor

    Plasmid

    #176363

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White, Barbara Stranger

    Insert

    OSR1 (OSR1 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pZNF627-donor

    Plasmid

    #176365

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White, Barbara Stranger

    Insert

    ZNF627 (ZNF627 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pZNF79-donor

    Plasmid

    #176371

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White, Barbara Stranger

    Insert

    ZNF79 (ZNF79 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSNAPC5-donor

    Plasmid

    #176341

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White, Barbara Stranger

    Insert

    SNAPC5 (SNAPC5 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge