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Plasmid#50725Purposeoverexpression of the human TPI1 Ile170Thr allele in E.coliDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTags6xHIS tagExpressionBacterialMutationchanged Isoleucine 170 to ThreoninePromoterT7Available sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
TPI in pCW57_tTA_Blast
Plasmid#154209PurposeDox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of TPIDepositorInsertTPI1 (TPI1 Human)
UseLentiviralTagsNoneExpressionMammalianMutationPromoterTRE repeats (dox off)Available sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
TPI-PTC160
Plasmid#130698PurposeExpresses TPI-PTC160 NMD reporter (It has a Premature termination codon (PTC) at 160th Amino acid)DepositorInsertTPI-PTC160 (TPI1 Human)
UseTagsExpressionMammalianMutationPTC160PromoterPcmvAvailable sinceDec. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
TPI-PTC1
Plasmid#130699PurposeExpresses TPI-PTC1 NMD reporter (It has a Premature termination codon (PTC) at First Amino acid)DepositorInsertTPI-PTC1 (TPI1 Human)
UseTagsExpressionMammalianMutationPTC1PromoterPcmvAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI-TPI_PTC160-xrRNA-4H
Plasmid#108369PurposeExpresses TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertPTC160 TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
UseTagsExpressionMammalianMutationPTC160PromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI-TPI_WT-xrRNA-4H
Plasmid#108368PurposeExpresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertWild type TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-TPI_PTC160-xrRNA-4H
Plasmid#108378PurposeFRT integration of TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertPTC160 TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-TPI_WT-xrRNA-4H
Plasmid#108377PurposeFRT integration of wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertWild type TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3.2mar(T->G)-GFP
Plasmid#63695Purpose3.2 kb marine armor plate enhancer with a single T->G bp change driving GFP reporterDepositorInsert3.2 kb marine stickleback armor plate enhancer with a single T->G base pair change
UseTagsEGFPExpressionMammalianMutationT->G bp changePromoterhsp70Available sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRMC2-msfGFP
Plasmid#194913PurposeTetracycline inducible expression of msfGFP in StaphylococciDepositorInsertShine Dalgarno Sequence followed by monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…TagsExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
HP21-TPI-WT-no_introns
Plasmid#131044PurposeExpresses TPI-Wildtype NMD reporter without introns and with a 21 nt hairpin in 5'UTRDepositorInsertTPI-Wildtype-no introns (TPI1 Human)
UseTagsExpressionMammalianMutationPromoterPcmvAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
NG0886 pCI-TPI-WT-4H
Plasmid#65801PurposeNMD reporter mRNADepositorInsertTPI (TPI1 Human)
UseTags4H (4 copies of short beta-globin 3'UTR for …ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
NG0750 pCI-TPI-PTC48-4H
Plasmid#65803PurposeNMD reporter mRNADepositorInsertTPI (TPI1 Human)
UseTags4H (4 copies of short beta-globin 3'UTR for …ExpressionMammalianMutationpremature stop codon at 48PromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
HP21-TPI-PTC1
Plasmid#131043PurposeExpresses TPI-PTC1 NMD reporter (It has a Premature termination codon (PTC) at First Amino acid) with a 21 nt hairpin in 5'UTRDepositorInsertTPI-PTC1 (TPI1 Human)
UseTagsExpressionMammalianMutationPTC1PromoterPcmvAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
NG1237 pCI-TPI-PTC160-4H
Plasmid#65804PurposeNMD reporter mRNADepositorInsertTPI (TPI1 Human)
UseTags4H (4 copies of short beta-globin 3'UTR for …ExpressionMammalianMutationpremature stop codon at 160PromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCI-TPI_WT-xrRNA-4MS2-4H
Plasmid#108370PurposeExpresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag); 4MS2 tethering sites downstream of xrRNA and allows detection via northern blot (4H binding sites)DepositorInsertWild type TPI reporter with 4MS2 binding sites downstream of MVE xrRNA and 4H probe binding sites (TPI1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI-TPI_WT-4MS2-xrRNA-4H
Plasmid#108371PurposeExpresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag); 4MS2 tethering sites upstream of xrRNA and allows detection via northern blot (4H binding sites)DepositorInsertWild type TPI reporter with 4MS2 binding sites upstream of MVE xrRNA and 4H probe binding sites (TPI1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
NG0888 pCI-TPI-PTC48-HBB
Plasmid#65802PurposeNMD reporter mRNADepositorInsertTPI (TPI1 Human)
UseTagsHBB (beta-globin 3'UTR for probe binding)ExpressionMammalianMutationpremature stop codon at 48PromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
NG0911 pCI-TPI-SMG5(short)-4H
Plasmid#65805PurposeNMD reporter mRNADepositorInsertTPI (TPI1 Human)
UseTags4H (4 copies of short beta-globin 3'UTR for …ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRMC2-tat:msfGFP
Plasmid#194915PurposeTetracycline inducible expression of msfGFP fused to Tat signal peptide in StaphylococciDepositorInsertShine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…TagsExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only