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lentiCRISPR v2-Nr4a1sgRNA1

Plasmid

#160945

Purpose

Guide RNA 1 to generate Nr4a1 knockout by CRISPR

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nr4a1 gRNA 1 (Nr4a1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDY1622 NOLC1 sgRNA 1

Plasmid

#234833

Purpose

sgRNA 1 for NOLC1 STITCHR insertion

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

Insert

NOLC1 sgRNA (NOLC1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCW57.1-Gata6-3xT7

Plasmid

#200889

Purpose

Inducible lentiviral expression of Gata6

Depositor

Kai Ge

Article

Xie et al Nat Genet. 2023 Apr 3. doi: 10.1038/s415

Insert

Gata6 (Gata6 Mouse)

Use

Lentiviral; Doxycycline inducible

Tags

3xT7

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet ON

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-Nr4a1sgRNA2

Plasmid

#160946

Purpose

Guide RNA 2 to generate Nr4a1 knockout by CRISPR

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nr4a1 gRNA 2 (Nr4a1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX304-ARF5-T161A-mNeonGreen

Plasmid

#162026

Purpose

Fast cycling ARF mutant

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

ARF5 (ARF5 Human)

Use

Lentiviral

Tags

Expression

Mutation

T161A

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1D V5-His-TOPO-KGA.wt

Plasmid

#110332

Purpose

Mammalian expression of glutaminase isoform KGA (wild type) with V5 and 6xHis tags

Depositor

Sandra Martha Gomes Dias

Article

Cassago et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

Insert

KGA glutaminase (GLS Human)

Use

Tags

6xHis and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-HA-Nr4a1-AA

Plasmid

#160942

Purpose

Generation of retrovirus for the overexpression of Nr4a1 mutant (287C 288EAA)

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nr4a1 (Nr4a1 Mouse)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

Change Cys 287 Glu 288 into Ala Ala

Promoter

MSCV-LTR

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-HA-Nr4a1-Zf2

Plasmid

#160944

Purpose

Generation of retrovirus for the overexpression of Nr4a1 mutant (zinc finger 2 deletion)

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nr4a1 (Nr4a1 Mouse)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

Deletion of Zinc finger 2 (C306 to C330)

Promoter

MSCV-LTR

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-HA-Nr4a1-Zf1

Plasmid

#160943

Purpose

Generation of retrovirus for the overexpression of Nr4a1 mutant (zinc finger 1 deletion)

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nr4a1 (Nr4a1 Mouse)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

Deletion of Zinc finger 1 (C270 to C290)

Promoter

MSCV-LTR

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pQP-AR10

Plasmid

#138980

Purpose

Endogenous RAS regulation using light-controlled intrabody

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

BglII-BphP1-NES-T2A- iB(RAS)-NES-mCherry-QPAS1- NLS-NotI

Use

Tags

Expression

Mutation

Promoter

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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PP160

Plasmid

#78992

Purpose

Supplemental Figure 4. Promoter GAP2. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PP155

Plasmid

#78994

Purpose

Supplemental Figure 4. Promoter GAP4. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PP163

Plasmid

#78996

Purpose

Supplemental Figure 4. Promoter GAP6. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PP245

Plasmid

#78965

Purpose

Promoter: GAP, minimal Promoter: AOX1. Figure 4. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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PP259

Plasmid

#78961

Purpose

Promoter: scTEF1, minimal Promoter: mCYC. Figure 4. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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PP273

Plasmid

#78971

Purpose

Promoter: ppTEF1, minimal Promoter: GCW14. Figure 4. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PP257

Plasmid

#78963

Purpose

Promoter: GAP, minimal Promoter: mCYC. Figure 4. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PP277

Plasmid

#78967

Purpose

Promoter: scTEF1, minimal Promoter: GCW14. Figure 4. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PP190

Plasmid

#78969

Purpose

Promoter: ppTEF1, minimal Promoter: AOX1. Figure 4. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pGAPTrap-IRESMuro

Plasmid

#82336

Purpose

Base vector targeting genes to the GAPDH locus of human cells. Inserted genes are expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

Depositor

Ed Stanley

Article

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

IRESMuro

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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