We narrowed to 18,365 results for: Tec

1. pcDNA3.1(+)-ExRai-AKAR

   Plasmid

   #118405

   Purpose

   cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AKAR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)-FKBP-(GGS)X6-HaloTag C58

   Plasmid

   #171428

   Purpose

   Expression of FKBP-(GGS)X6-HaloTag C58 in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   FKBP-(GGS)X6-HaloTag C58
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. NLS-SpCas9mSA-His

   Plasmid

   #182951

   Purpose

   Express SpCas9mSA in bacteria

   Depositor

   Wen Xue

   Article

   Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

   Insert

   NLS-SpCas9mSA-His
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)-HaloTag N58-(GGS)X6-c-Jun

   Plasmid

   #171438

   Purpose

   Expression of HaloTag N58-(GGS)X6-c-Jun in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   HaloTag N58-(GGS)X6-c-Jun
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSH1/M-FRBl2-E

   Plasmid

   #15289

   Depositor

   David Spencer

   Article

   Li et al Gene Ther. 2002 Feb . 9(4):233-44.

   Insert

   FRAP-rapamycin binding domain (MTOR Human)
   

   Use

   Tags

   HA epitope and Myristoylation-targeting domain c-…

   Expression

   Mammalian

   Mutation

   res. 2025-2113, T2098L

   Promoter

   Available since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMM146

   Plasmid

   #127218

   Purpose

   BeYDV replicon with constitutive Luc expression, BBM, sgRNA targeting PDS

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   Luc, BBM, sgRNA
   

   Use

   Tags

   Expression

   Plant

   Mutation

   codon optimized CDSs

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMM135

   Plasmid

   #127216

   Purpose

   BeYDV replicon with constitutive Luc expression, WUS2, sgRNA targeting PDS

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   Luc, WUS2, sgRNA
   

   Use

   Tags

   Expression

   Plant

   Mutation

   codon optimized CDSs

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUCBB-ctH6-eGFP

   Plasmid

   #32562

   Depositor

   Claudia Schmidt-dannert

   Article

   Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

   Insert

   eGFP (eGFP )
   

   Use

   Synthetic Biology

   Tags

   6 His tag with Thrombin site

   Expression

   Mutation

   Promoter

   Consitutive lac

   Available since

   Oct. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-hsyn-GRAB_ATP1.0mut

   Plasmid

   #167578

   Purpose

   Express the ATP-insensitive control sensor GRAB_ATP1.0mut in neurons

   Depositor

   Yulong Li

   Article

   Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

   Insert

   ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBT13-splitC

    Plasmid

    #122596

    Purpose

    clone M13 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    M13 bacteriophage genes
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG-Cre

    Plasmid

    #125574

    Purpose

    Expresses Cre under the CAG promoter in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTK313

    Plasmid

    #59570

    Purpose

    TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak EGFP-PEST ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP XbaI attB1 Kozak EGFP-PEST ochre attB2 AscI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FKBP_LD 0_TEV

    Plasmid

    #58877

    Purpose

    MESA protease chain with Rapamycin FKBP ectodomain and no linker domain

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA protease chain with FKBP rapamycin-binding ectodomain, TEV protease and mCherry fusion
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. UBQ10:sXVE:(MCS)-S11

    Plasmid

    #108179

    Purpose

    Binary vectors used for the inducible expression of tripartite split-sfGFP components in plants

    Depositor

    Tzu-Yin Liu

    Article

    Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    UBQ10:sXVE:

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pC0075 TtCsm6 His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115270

    Purpose

    Expresses TtCsm6 from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    TtCsm6
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-Cas9-162A

    Plasmid

    #84918

    Purpose

    for in vitro transcription of Cas9 with polyA tail encoded

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Cas9
    

    Use

    Tags

    Flag, NLS, and polyA tail

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. PclpB riboswitch GFP pZa

    Plasmid

    #170078

    Purpose

    GFP expression under the control of E. coli clpB promoter engineered with riboregulators

    Depositor

    Urartu Seker

    Article

    Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

    Insert

    Green fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PclpB

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTK317

    Plasmid

    #59571

    Purpose

    TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(16) 2xK-turn Kozak EGFP attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP(16) 2xK-turn Kozak EGFP attB2 AscI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUPD2_miniDFR (GB2566)

    Plasmid

    #160595

    Purpose

    Minimal promoter of slDFR gene containing 62bp upstream the transcription start site and the 5'UTR region

    Depositor

    Diego Orzaez

    Article

    Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

    Insert

    miniDFR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC-H1-gRNA

    Plasmid

    #61089

    Purpose

    CRISPR/Cas9 system gRNA cloning

    Depositor

    Shenglin Huang

    Article

    Zheng et al Biotechniques. 2014 Sep 1;57(3):115-24.

    Insert

    H1 promoter; gRNA sequences
    

    Use

    CRISPR; /cas9 grna cloning vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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