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pET28.His.3C.86b.20D3Q.Tau2N4R

Plasmid

#226401

Purpose

Expresses his-tagged, 3C-cleavable, 86b (split luciferase fragment)-tagged 20D3Q mutant 2N4R tau for recombinant protein production in bacteria

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

His.3C.86b

Expression

Bacterial

Mutation

20D3Q, Tau2N4R

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-CREM-Brd 328

Plasmid

#219258

Purpose

Transcription factor CREM with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 21;17(1):101457. doi

Insert

CREM (CREM Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-CREB3-Brd 275

Plasmid

#219217

Purpose

Transcription factor CREB3 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 21;17(1):101457. doi

Insert

CREB3 (CREB3 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-HOXB2-Brd 250

Plasmid

#219193

Purpose

Transcription factor HOXB2 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 21;17(1):101457. doi

Insert

HOXB2 (HOXB2 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-TARPGamma2/4/8 [N245/36R-2b]

Plasmid

#220426

Purpose

Mammalian Expression Plasmid of anti-TARPGamma2/4/8 (Rat) subclass-switched IgG2b R-mAb. Derived from hybridoma N245/36.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-TARPGamma2/4/8 (Rattus norvegicus) recombinant mouse monoclonal antibody. (Tarp Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac dual_Human full length _CDK7 (S164D, T170E)_del327-346

Plasmid

#217012

Purpose

Expression of CDK7 in insect cells

Depositor

Manjeet Mukherjee

Article

Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018

Insert

CDK7 (S164D, T170E)_del327-346 (CDK7 Human)

Tags

TEV cleavable his tag

Expression

Insect

Mutation

(S164D, T170E)_del327-346

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac dual_Human full length CDK7(W132R, S164D, T170E)

Plasmid

#217013

Purpose

Expression of CDK7 in insect cells

Depositor

Manjeet Mukherjee

Article

Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018

Insert

CDK7(W132R, S164D, T170E) (CDK7 Human)

Tags

TEV cleavable his tag

Expression

Insect

Mutation

CDK7(W132R, S164D, T170E)

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(POU5F1_g1)-PGKpuroBFP-W

Plasmid

#211980

Purpose

Express gRNA against POU5F1 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting POU5F1 (POU5F1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(POU5F1_g15-14)-PGKpuroBFP-W

Plasmid

#211981

Purpose

Express gRNA against POU5F1 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting POU5F1 (POU5F1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(JARID2_5-5)-PGKpuroBFP-W

Plasmid

#211967

Purpose

Express gRNA against JARID2 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting JARID2 (JARID2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-hSOX2-C17

Plasmid

#206385

Purpose

Expresses human SOX2-C17 in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

Insert

SOX2-C17 chimera (SOX2, SOX17 Human)

Use

Lentiviral

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-CSF3R-COMP5AP-AviTag-9xHis

Plasmid

#157487

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CSF3R (CSF3R Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-CRLF1-COMP5AP-AviTag-9xHis

Plasmid

#157378

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CRLF1 (CRLF1 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-HAPLN4-COMP5AP-AviTag-9xHis

Plasmid

#157373

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

HAPLN4 (HAPLN4 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-IL1RAPL1-COMP5AP-AviTag-9xHis

Plasmid

#157342

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

IL1RAPL1 (IL1RAPL1 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-HAPLN4-Fc(DAPA)-AviTag-6xHis

Plasmid

#156809

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

HAPLN4 (HAPLN4 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-CRLF1-Fc(DAPA)-AviTag-6xHis

Plasmid

#156814

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CRLF1 (CRLF1 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pX335_U6-Chimeric_BB-CBh-hSpCas9n(D10A)_SOX17_Ex2_KI

Plasmid

#195502

Purpose

Cas9 nickase expression vector bearing a sgRNA targeting Exon 2 of human SOX17

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

sgRNA (SOX17 Human)

Use

CRISPR

Tags

HA

Expression

Mammalian

Promoter

U6

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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dCas9-p300 PHD-HAT

Plasmid

#179545

Purpose

encodes S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) driven by EF-1alpha promoter

Depositor

Isaac Hilton

Article

Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

Insert

S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) (EP300 Human)

Use

Lentiviral

Tags

Flag Tag

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

EF-1alpha

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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dCas9-p300 RING-PHD-HAT

Plasmid

#179546

Purpose

encodes S. pyogenes dCas9 with c-terminal fusion of human p300 RING-PHD-HAT domain (aa 1155-1664) driven by EF-1alpha promoter

Depositor

Isaac Hilton

Article

Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

Insert

S. pyogenes dCas9 with c-terminal fusion of human p300 RING-PHD-HAT domain (aa 1155-1664) (EP300 Human)

Use

Lentiviral

Tags

Flag Tag

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

EF-1alpha

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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