We narrowed to 18,395 results for: tec

1. pSplit2-NO-PRPF6

   Plasmid

   #51740

   Purpose

   Express LucN-PRPF6 fusion protein in mammalian cells

   Depositor

   Hiroyoshi Ariga

   Article

   Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

   Insert

   PRP6 (PRPF6 Human)
   

   Use

   Tags

   FLAG and Luciferase (1-398aa)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfB6364

   Plasmid

   #106151

   Purpose

   EasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying D-serine marker, integration into Yarrowia lipolytica ku70 locus, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Ca-FLARE (TF)

   Plasmid

   #92213

   Purpose

   Transcription factor component of FLARE

   Depositor

   Alice Ting

   Article

   Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

   Insert

   NRX-TM-Nav1.6 -CaMbp(M2)-eLOV-TEVcs(ENLYFQ▲M)-tTA-VP16
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   synapsin

   Available since

   June 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. mVenus-P115-C1

   Plasmid

   #112931

   Purpose

   p115RhoGEF tagged with mVenus for detection of Ga13 activity

   Depositor

   Dorus Gadella

   Article

   Mastop et al PLoS One. 2018 Mar 5;13(3):e0193705. doi

   Insert

   p115 RhoGEF
   

   Use

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTS1017-Tier1-OTetO7-PCMVmin-SEAP

   Plasmid

   #169586

   Purpose

   Tier-1 vector encoding PTetO7-driven SEAP (OTetO7-PCMVmin-SEAP-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   tetracycline-controlled SEAP expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO7-PCMVmin

   Available since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. LbCas12a-D156R

   Plasmid

   #213744

   Purpose

   For circular RNA-mediated prime editor using LbCas12a-D156R in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   LbCas12a
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   D156R

   Promoter

   CMV

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDonor14-ShoHELIX-KanR (BO4)

   Plasmid

   #181795

   Purpose

   pDonor for ShoHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   KanR, R6K origin of replication
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmCherry-T2A-moxMaple3

   Plasmid

   #120875

   Purpose

   Mammalian expression of mCherry and moxMaple3

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

   Inserts

   mCherry
   moxMaple3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   from mMaple3, moxMaple3 was engineered by replaci…

   Promoter

   Available since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EFS-DIO-GRAB_ATP1.0

   Plasmid

   #167581

   Purpose

   Express the ATP sensor GRAB_ATP1.0 in Cre positive cells

   Depositor

   Yulong Li

   Article

   Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

   Insert

   ATP sensor GRAB_ATP1.0 (P2RY1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. TCARZ-SV40-GFP-PAmin-GF3-R4A22

    Plasmid

    #203769

    Purpose

    For production of template RNA for use in PRINT, GFP under SV40 promoter

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    PRINT template RNA for GFP
    

    Use

    Ivt template

    Tags

    Expression

    Mutation

    Promoter

    SV40

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDY1461_SERPINA1_CCA32_5avidity Cre

    Plasmid

    #193193

    Purpose

    Express Cre conditional upon RNA transcript

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

    Insert

    Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQC.Flip-nluc(LgBiT1-8)CP[TEV]

    Plasmid

    #200122

    Purpose

    Retroviral expression of Flip-nluc(LgBiT1-8)CP[TEV] protease reporter and T2A mCherry in mammalian cells

    Depositor

    Eiji Morita

    Article

    Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

    Insert

    Flip-nluc(LgBiT1-8)CP[TEV] T2A mCherry
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAC1430

    Plasmid

    #127981

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pH-A3A-PBE

    Plasmid

    #119774

    Purpose

    Conversion of C to T in rice plants transformation

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-NLS-UGI-NLS
    

    Use

    Tags

    NLS (flanking UGI)

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    maize Ubiquitin-1

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBT100.106b

    Plasmid

    #122601

    Purpose

    clone APOBEC1 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    APOBEC1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCfB6630

    Plasmid

    #106160

    Purpose

    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6371 into Yarrowia lipolytica chromosomal location IntC_3, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Insert

    unknown
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+)-ExRai-AKAR

    Plasmid

    #118405

    Purpose

    cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AKAR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETM6-HpaBC

    Plasmid

    #71260

    Purpose

    Expresses E. coli HpaBC in monocistronic form from ePathBrick vector, pETM6

    Depositor

    Mattheos Koffas

    Article

    Jones et al Biotechnol Prog. 2015 Oct 21. doi: 10.10

    Inserts

    HpaB
    HpaC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)-FKBP-(GGS)X6-HaloTag C58

    Plasmid

    #171428

    Purpose

    Expression of FKBP-(GGS)X6-HaloTag C58 in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    FKBP-(GGS)X6-HaloTag C58
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCfB6371

    Plasmid

    #106153

    Purpose

    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6630, integration into Yarrowia lipolytica chromosomal location IntC_3, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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