We narrowed to 23,593 results for: promoter

1. pscALPS gag-gfp/deltavpx

   Plasmid

   #115807

   Purpose

   SFFV promoter expresses gag-gfp fusion with no ORF after CypA promoter

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   gag-gfp
   

   Use

   Lentiviral

   Promoter

   SFFV

   Available Since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-hBcan

   Plasmid

   #18966

   Depositor

   Mariano Viapiano

   Article

   Hu et al J Biol Chem. 2008 Sep 5;283(36):24848-59

   Insert

   Brevican (BCAN Human)
   

   Expression

   Mammalian

   Available Since

   Dec. 12, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1 HA-rnSOX9

   Plasmid

   #62972

   Purpose

   Expresses HA-tagged rat SOX9

   Depositor

   Michael Ruppert

   Article

   Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

   Insert

   SOX9 (Sox9 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

4. His10-PS-SNAPf (XSB522)

   Plasmid

   #78512

   Purpose

   Construct for expressing His10 - SNAPf in E. Coli

   Depositor

   Ron Vale

   Article

   Su et al Science. 2016 Apr 29;352(6285):595-9. do

   Insert

   SNAPf
   

   Tags

   His10 followed by a prescission protease claevage…

   Expression

   Bacterial

   Available Since

   June 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACT2-CAS9c

   Plasmid

   #51055

   Purpose

   Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.

   Depositor

   Yunde Zhao

   Article

   Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

   Insert

   Cas9c
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Bacterial and Yeast

   Promoter

   yeast ADH1 promoter

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. hOC-GFPtpz

   Plasmid

   #110207

   Purpose

   Expresses GFP in mature bone cells.

   Depositor

   David Rowe

   Article

   Bilic-Curcic et al Genesis. 2005 Oct;43(2):87-98. doi: 10.1

   Insert

   eGFPtopaz
   

   Tags

   None

   Expression

   Mammalian

   Promoter

   Human osteocalcin (BGLAP)

   Available Since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. NK73

   Plasmid

   #176609

   Purpose

   To test bead engulfment of macrophages.

   Depositor

   Meghan Morrissey

   Article

   Kern et al eLife. 2021 Jun 3;10. pii: 68311. doi: 1

   Insert

   SNAP-CD86-FCERG (CD86 Human, Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   n/a

   Available Since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-E1.1-RFP

   Plasmid

   #22928

   Depositor

   Edward Callaway

   Article

   Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

   Insert

   E1.1 binding site from Scardigli et al., 2003 with minimal CMV
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   May 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-AAVS1-Ef1a-Zeo

   Plasmid

   #227268

   Purpose

   Empty AAVS1 targeting donor for the insertion of Zeo and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRE2-Bla(HA–RILP)

    Plasmid

    #102425

    Purpose

    HA tag fused to the N-terminus of RILP for the expression in mammalian cells.

    Depositor

    Steven Weinman

    Article

    Wozniak et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    Rab interacting lysosomal protein (RILP Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    Tet-responsive

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-nucGFP11-3xControlgRNA

    Plasmid

    #224568

    Purpose

    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    GFP11-H2B
    

    Use

    CRISPR

    Tags

    Histone H2B (nuclear localization)

    Mutation

    Codon optimized

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-nDsRedFL-intron

    Plasmid

    #177804

    Purpose

    An intron is inserted into the DsRed gene with a nuclear transfer signal and a 3xFlag tag. The intron can be digested with EcoRV and any pre-miRNA can be inserted.

    Depositor

    Rikako Sanuki

    Article

    Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

    Insert

    intron
    

    Expression

    Mammalian

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMADM-beta

    Plasmid

    #36891

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    Beta-Geo
    thymidine kinase
    

    Expression

    Mammalian

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available Since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZmUbi-5'UTR

    Plasmid

    #154057

    Purpose

    Level 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Wheat construct)

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Insert

    Promoter and 5' UTR Ubiquitin (Zea mays)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    N/A

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFA6a-kanMX6-PGAL1

    Plasmid

    #41605

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Available Since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT7-SpCas9_sgRNA-site1 (RTW443)

    Plasmid

    #160136

    Purpose

    T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #1

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    SpCas9 sgRNA with spacer #1 (spacer=GGGCACGGGCAGCTTGCCGG)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Promoter

    T7

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. GG-EBNA-OCT4

    Plasmid

    #69537

    Purpose

    Episomal plasmid encoding 5 gRNAS targeting human OCT4 promoter

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

    Insert

    5 concatenated gRNA transcriptional cassettes targeting OCT4
    

    Use

    CRISPR

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. 3xFLAG-dCas9/pMXs-IG

    Plasmid

    #51258

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS (nuclear localization signal)

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pL0-MtBCP1Pro

    Plasmid

    #159415

    Purpose

    Medicago truncatula BCP1 promoter sequence (1108 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.

    Depositor

    Sam Brockington

    Article

    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

    Insert

    MtBCP1 Promoter
    

    Use

    Puc19-derived

    Expression

    Bacterial

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHes1-GFP (CC#109)

    Plasmid

    #15133

    Depositor

    Connie Cepko

    Article

    Furukawa et al Neuron. 2000 May . 26(2):383-94.

    Insert

    Hes1 (Hes1 Rat)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only