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pMXS-IRES-BLAST flag-CAD S1345A

Plasmid

#188119

Purpose

Expresses C-terminal flag-tagged human CAD S1345A in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD T1348A

Plasmid

#188120

Purpose

Expresses C-terminal flag-tagged human CAD T1348A in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pSLED.CaRPv1

Plasmid

#193018

Purpose

Excitatory/Inhibitory neuron calcium indicator (hSyn promoter, Synrg exon, jRGECO1a/jGCaMP7b)

Depositor

Jonathan Ling

Article

Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

Insert

Bichromatic calcium indicator (jGCaMP7b and jRGECO1a)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-gRNA(SFTPCI73Tcorr)-SpCas9(BB)-2A-GFP

Plasmid

#187647

Purpose

Encodes gRNA to correct the SFTPC I73T mutation

Depositor

Darrell Kotton

Article

Alysandratos et al Cell Rep. 2021 Aug 31;36(9):109636. doi:

Insert

I73T gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5 rtTA BirA eIF4G +MIC

Plasmid

#158790

Purpose

pcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 including the microexon for generation of N2A FlpIn cell lines for BioID

Depositor

Benjamin Blencowe

Article

Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

Insert

eIF4G1 (with microexon)

Use

Tags

3xFlag and BirA*

Expression

Mammalian

Mutation

Promoter

miniCMV

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_ARSI_WT

Plasmid

#81898

Purpose

Gateway Donor vector containing ARSI , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ARSI (ARSI Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_GPR141_WT

Plasmid

#81876

Purpose

Gateway Donor vector containing GPR141 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

GPR141 (GPR141 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CNBD1_WT

Plasmid

#81877

Purpose

Gateway Donor vector containing CNBD1 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CNBD1 (CNBD1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HERPUD2_WT

Plasmid

#81880

Purpose

Gateway Donor vector containing HERPUD2 ,part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HERPUD2 (HERPUD2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

A108T

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IKZF5_WT

Plasmid

#81855

Purpose

Gateway Donor vector containing IKZF5 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IKZF5 (IKZF5 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

M204V; p.415_418delQHNQ; p.419_420-insITNID

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_C20orf72_WT

Plasmid

#81806

Purpose

Gateway Donor vector containing C20ORF72 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

C20orf72 (MGME1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_GZMA_WT

Plasmid

#81792

Purpose

Gateway Donor vector containing GZMA , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

GZMA (GZMA Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Y5N

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HRH4_WT

Plasmid

#81786

Purpose

Gateway Donor vector containing HRH4 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HRH4 (HRH4 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRT26_WT

Plasmid

#81763

Purpose

Gateway Donor vector containing KRT26 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRT26 (KRT26 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_EGFR_p.P622S

Plasmid

#81721

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

EGFR (EGFR Human)

Use

Gateway entry vector

Tags

Expression

Mutation

P622S

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HTR3B_p.I118T

Plasmid

#81730

Purpose

Gateway Donor vector containing HTR3B, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HTR3B (HTR3B Human)

Use

Gateway entry vector

Tags

Expression

Mutation

I118T

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HTR4_p.T105M

Plasmid

#81731

Purpose

Gateway Donor vector containing HTR4 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HTR4 (HTR4 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

T105M

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAP4K3_p.Q473H

Plasmid

#81706

Purpose

Gateway Donor vector containing MAP4K3, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAP4K3 (MAP4K3 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Q473H

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_BRAF_p.G466A

Plasmid

#81707

Purpose

Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BRAF (BRAF Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G466A

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_EGFR_p.G719D

Plasmid

#81708

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

EGFR (EGFR Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G719D

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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