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pAV-U6+27-Linear-3_PolyU(1)_Tornado-Corn

Plasmid

#159503

Purpose

Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-3_PolyU(1)

Expression

Mammalian

Promoter

U6-27

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-2_PolyU(7)_Tornado-Corn

Plasmid

#159493

Purpose

Tests for the impact of 7 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-2_PolyU(7)

Expression

Mammalian

Promoter

U6-27

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-2_PolyU(4)_Tornado-Corn

Plasmid

#159490

Purpose

Tests for the impact of 4 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-2_PolyU(4)

Expression

Mammalian

Promoter

U6-27

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-2_PolyU(2)_Tornado-Corn

Plasmid

#159488

Purpose

Tests for the impact of 2 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-2_PolyU(2)

Expression

Mammalian

Promoter

U6-27

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-U6+27-Linear-1_PolyU(4)_Tornado-Corn

Plasmid

#159482

Purpose

Tests for the impact of 4 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(4)

Expression

Mammalian

Promoter

U6-27

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-U6+27-Linear-1_PolyU(5)_Tornado-Corn

Plasmid

#159483

Purpose

Tests for the impact of 5 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(5)

Expression

Mammalian

Promoter

U6-27

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-U6+27-Linear-1_PolyU(6)_Tornado-Corn

Plasmid

#159484

Purpose

Tests for the impact of 6 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(6)

Expression

Mammalian

Promoter

U6-27

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-U6+27-Linear-1_PolyU(7)_Tornado-Corn

Plasmid

#159485

Purpose

Tests for the impact of 7 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(7)

Expression

Mammalian

Promoter

U6-27

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-U6+27-Linear-1_PolyU(8)_Tornado-Corn

Plasmid

#159486

Purpose

Tests for the impact of 8 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(8)

Expression

Mammalian

Promoter

U6-27

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-Avi-Coh (E154C)-HIS

Plasmid

#153447

Purpose

E. coli expression of Rc. CohE construct with E154C mutation.

Depositor

Michael Nash

Article

Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

Insert

Rc.CohE

Tags

6xHis and Avi-tag

Expression

Bacterial

Mutation

E154C mutation on CohE

Promoter

T7 promoter

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pEM03

Plasmid

#135417

Purpose

Expression of Hygromycin resistance from Spizellomyces Hsp70 promoter

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

SpunHsp70pr

Expression

Bacterial and Yeast

Promoter

Hsp70pr

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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Y365A-GFP Donor for iMTr EED

Plasmid

#131325

Purpose

Donor to generate i-MT-r system

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

i-MT-r.Donor

Use

CRISPR and Mouse Targeting

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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A365Y-GFP Donor for iWTr EED

Plasmid

#131324

Purpose

Donor to generate i-WT-r system

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

i-WT-r.Donor

Use

CRISPR and Mouse Targeting

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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EED-KO-gRNA-2

Plasmid

#131322

Purpose

gRNA to knockout EED. Use with EED-KO-gRNA-1

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

EED-KO-gRNA-2

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only
S3_007_026_MV1

Plasmid

#134419

Purpose

pYTKMV1-pTDH3_ NLS_FdeR-N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pTDH3

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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S3_008_026_MV1

Plasmid

#134420

Purpose

pYTKMV1-pRPL18B_ NLS_FdeR_N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pRPL18B

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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S3_009_026_MV1

Plasmid

#134421

Purpose

pYTKMV1-pREV1_ NLS_FdeR-N_2NLS-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pREV1

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
S3_007_026_MV2

Plasmid

#134424

Purpose

pYTKMV2-pTDH3_ NLS_FdeR-N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pTDH3

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
S3_007_026_096

Plasmid

#134410

Purpose

pYTK096-pTDH3_ NLS_FdeR-N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGPM1 and pTDH3

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
S3_008_026_096

Plasmid

#134411

Purpose

pYTK096-pRPL18B_ NLS_FdeR_N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGPM1 and pRPL18B

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
S3_009_026_096

Plasmid

#134412

Purpose

pYTK096-pREV1_ NLS_FdeR-N_2NLS-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pREV1

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
S3_001_026_096

Plasmid

#134402

Purpose

pYTK096-pTDH3_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGPM1 and pTDH3

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
S3_004_026_096

Plasmid

#134407

Purpose

pYTK096-pTDH3_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGPM1 and pTDH3

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
S3_002_026_MV1

Plasmid

#134414

Purpose

pYTKMV1-pRPL18B_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pRPL18B

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
S3_006_026_096

Plasmid

#134409

Purpose

pYTK096-pREV1_FdeR-N_2NLS_tPGK1- pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGPM1 and pREV1

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
S3_006_026_096

Plasmid

#134418

Purpose

pYTK096-pREV1_FdeR-N_2NLS_tPGK1- pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pREV1

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
S3_004_026_MV2

Plasmid

#134423

Purpose

pYTKMV2-pTDH3_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pTDH3

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
S3_003_026_096

Plasmid

#134406

Purpose

pYTK096-pREV1_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGPM1 and pREV1

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
S3_005_026_MV1

Plasmid

#134417

Purpose

pYTKMV1-pRPL18B_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pRPL18B

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
S3_003_026_MV1

Plasmid

#134415

Purpose

pYTKMV1-pREV1_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pREV1

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge