We narrowed to 22,830 results for: Sis

1. pKLBac-FluB-trimer

   Plasmid

   #229859

   Purpose

   Influenza B/Memphis/13/03 polymerase heterotrimer (self-cleaving polyprotein)

   Depositor

   Stephen Cusack

   Article

   Reich et al Nature. 2014 Dec 18;516(7531):361-6. doi

   Inserts

   PA, Influenza B (B/Memphis/13/03)
   PB1, Influenza B (B/Memphis/13/03)
   PB2, Influenza B (B/Los Angeles/1/02)
   

   Tags

   2x-Streptag and 8xHis-tag

   Expression

   Insect

   Available Since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PhyB-mCherry-Tom7

   Plasmid

   #66571

   Purpose

   Constitutively express mitochondrial localized PhyB in Saccharomyces cerevisiae

   Depositor

   Orion Weiner

   Article

   Jost et al ACS Synth Biol. 2015 Jun 2.

   Insert

   PhyB (PHYB Mustard Weed)
   

   Tags

   Tom7 and mCherry

   Expression

   Yeast

   Mutation

   PhyB portion contains aa's 1-908

   Promoter

   pADH1

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSH1/M-FRBl2-E

   Plasmid

   #15289

   Depositor

   David Spencer

   Article

   Li et al Gene Ther. 2002 Feb . 9(4):233-44.

   Insert

   FRAP-rapamycin binding domain (MTOR Human)
   

   Tags

   HA epitope and Myristoylation-targeting domain c-…

   Expression

   Mammalian

   Mutation

   res. 2025-2113, T2098L

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCK019

   Plasmid

   #105378

   Purpose

   Synthetic biology

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   promotor EXP7 (At1g12560; 750bp)
   

   Use

   Synthetic Biology

   Mutation

   BsaI/ BpiI restriction sites removed

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pVP2A-PIF6

   Plasmid

   #73369

   Purpose

   Expresses Adeno-associated virus VP2 with PIF6 insert.

   Depositor

   Junghae Suh

   Article

   Gomez et al ACS Nano. 2015 Nov 30.

   Insert

   PIF6 (PIL2 Mustard Weed)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCfB6630

   Plasmid

   #106160

   Purpose

   EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6371 into Yarrowia lipolytica chromosomal location IntC_3, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Insert

   unknown
   

   Expression

   Yeast

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. hOC-GFPtpz

   Plasmid

   #110207

   Purpose

   Expresses GFP in mature bone cells.

   Depositor

   David Rowe

   Article

   Bilic-Curcic et al Genesis. 2005 Oct;43(2):87-98. doi: 10.1

   Insert

   eGFPtopaz
   

   Tags

   None

   Expression

   Mammalian

   Promoter

   Human osteocalcin (BGLAP)

   Available Since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CMV-Cx35-Halo-C-end

   Plasmid

   #71512

   Purpose

   Mammalian expression plasmid encoding perch Cx35 with a C-terminal HaloTag.

   Depositor

   John O'Brien

   Article

   Wang et al J Cell Sci. 2015 Nov 1;128(21):3888-97.

   Insert

   Connexin 35
   

   Tags

   HaloTag

   Expression

   Mammalian

   Available Since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHACK(Gal4)-DONR(T2A-Cas9)

   Plasmid

   #194768

   Purpose

   To insert T2A-Cas9 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Cas9 transgene.

   Depositor

   Chun Han

   Article

   Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

   Insert

   T2A-Cas9
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Tensin-2 WT

    Plasmid

    #201797

    Purpose

    Expresses wild-type Tensin-2 protein in MAC-Tag-C backbone

    Depositor

    Vesa Hytönen

    Article

    Turkki et al Sci Rep. 2024 Aug 27;14(1):19862. doi: 1

    Insert

    Tensin-2 (Tns2 Mouse)
    

    Tags

    BirA, HA, strepIII

    Expression

    Bacterial and Mammalian

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TetO7-HSP30UTR-nLucPEST-MS2(v4)

    Plasmid

    #199764

    Purpose

    Control construct of elongation reporter to quantify elongation of LacZ

    Depositor

    Brian Zid

    Article

    Guzikowski et al RNA Biol. 2022;19(1):636-649. doi: 10.10

    Insert

    NanolucPEST
    

    Expression

    Yeast

    Promoter

    TetO7

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p7133 pHAGE-P-CMVt-N-HA-GAW-PRKAR2B

    Plasmid

    #100160

    Purpose

    Expresses PRKAR2B

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    PRKAR2B (PRKAR2B Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p415TEF cyto roGFP2-Grx1(yeast)

    Plasmid

    #65004

    Purpose

    Yeast expression plasmid for roGFP2-Grx1; yeast codon optimized roGFP; yeast Grx1

    Depositor

    Tobias Dick

    Article

    Morgan et al Nat Chem Biol. 2013 Feb;9(2):119-25. doi

    Insert

    Glutaredoxin-1
    

    Tags

    roGFP2

    Expression

    Yeast

    Available Since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. RNF168-Halo HRD

    Plasmid

    #207080

    Purpose

    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    HaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
    

    Expression

    Mammalian

    Promoter

    None

    Available Since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMaCTag-P33

    Plasmid

    #120018

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with FLAG

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    FLAG
    

    Use

    Pcr template

    Tags

    FLAG

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSuper mouse Drosha2

    Plasmid

    #14775

    Depositor

    Tyler Jacks

    Article

    Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

    Insert

    Drosha shRNA (Drosha Mouse)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

17. SHC003BSD-DelGFP

    Plasmid

    #133301

    Purpose

    EGFP reporter was removed and the selection marker was modified as blasticidin in the construct of SHC003. This construct could be used for expression of a gene by lentiviral approach.

    Depositor

    Jingshi Shen

    Article

    Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TetO7-HSP30UTR-LacZ-PKTlinker-nLucPEST-MS2(v4)

    Plasmid

    #199763

    Purpose

    Elongation reporter construct to quantify elongation of LacZ

    Depositor

    Brian Zid

    Article

    Guzikowski et al RNA Biol. 2022;19(1):636-649. doi: 10.10

    Insert

    LacZ-nLucPEST
    

    Expression

    Yeast

    Promoter

    TetO7

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET23a-HsRPS27a

    Plasmid

    #69561

    Purpose

    Bacterial expression; in vitro transcription/translation (T7)

    Depositor

    Jorge Eduardo Azevedo

    Article

    Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

    Insert

    RPS27A (RPS27A Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pCAG-EGFP-C-IFT70B

    Plasmid

    #218729

    Purpose

    Expresses N-terminally EGFP-tagged IFT70B in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Takei et al Biol Open. 2018 Apr 30;7(5). pii: bio.03

    Insert

    IFT70B (TTC30B Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Halo-WIPI2 HRD

    Plasmid

    #207544

    Purpose

    Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous WIPI2 locus in human cells

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    HaloTag flanked by human WIPI2 locus sequences
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET26_MraYaa

    Plasmid

    #100166

    Purpose

    recombinant expression of target protein as an MBP-His tag fusionat the N-terminus of target protein

    Depositor

    Seok-Yong Lee

    Article

    Chung et al Science. 2013 Aug 30;341(6149):1012-1016

    Insert

    mraY (phospho-MurNAc-pentapeptide translocase)
    

    Tags

    MBP-His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Col1a-3'probe

    Plasmid

    #20731

    Depositor

    Rudolf Jaenisch

    Article

    Beard et al Genesis. 2006 Jan . 44(1):23-8.

    Insert

    Col1a-3'probe (Col1a1 Mouse)
    

    Expression

    Bacterial

    Available Since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBT44c

    Plasmid

    #138511

    Purpose

    P2

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    inteinC-dSpCas9-UGI
    

    Use

    Synthetic Biology

    Available Since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRU53

    Plasmid

    #167679

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::spCas9
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMADM-beta

    Plasmid

    #36891

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    Beta-Geo
    thymidine kinase
    

    Expression

    Mammalian

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available Since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDEST_6xUAS:miRFP670-cetn4

    Plasmid

    #192357

    Purpose

    zfCETN4 tagged with miRFP670, expressed under the UAS promoter

    Depositor

    Darren Gilmour

    Article

    Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

    Insert

    CETN4 (cetn4 Zebrafish)
    

    Tags

    miRFP670

    Expression

    Mammalian

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-myc-hIFITM3

    Plasmid

    #58461

    Purpose

    Expresses human IFITM3 with an N-terminal myc tag in mammalian cells.

    Depositor

    Jacob Yount

    Article

    Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

    Insert

    IFITM3 (IFITM3 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV IE

    Available Since

    Sept. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pET-DUET-1-mRhubarb720-HO

    Plasmid

    #141201

    Purpose

    Expresses mRhubarb720 fluorescent protein and heme oxygenase

    Depositor

    Elad Firnberg

    Article

    Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

    Inserts

    mRhubarb720
    heme oxygenase
    

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    R134H, L196Q, T202D, V203I, W309R, Q310A compared…

    Promoter

    T7

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMaCTag-Z22

    Plasmid

    #120065

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with SNAP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    SNAP
    

    Use

    Pcr template

    Tags

    SNAP

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Lenti-sgRNA(MS2)-puro-barcode

    Plasmid

    #89493

    Purpose

    Cloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seq

    Depositor

    Gary Hon

    Article

    Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

    Insert

    sgRNA (with MS2 loop)
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLAS3W-S100P

    Plasmid

    #125009

    Purpose

    express in mammalian cell

    Depositor

    Ruey-Hwa Chen

    Article

    Tan et al Oncogene. 2019 Jul;38(28):5612-5626. doi

    Insert

    S100P (S100P Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pUC18-H1 RNAi

    Plasmid

    #87355

    Purpose

    RNAi expression vector based on H1 promoter and pUC18 vector backbone

    Depositor

    Saroj Mathupala

    Article

    Mathupala et al Neurosurgery. 2004 Dec;55(6):1410-9; dis

    Insert

    H1 RNA Promoter from U87MG glioma cells
    

    Use

    RNAi; Shrna expression vector

    Expression

    Mammalian

    Promoter

    H1 RNA

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. mEos4a-C1

    Plasmid

    #54810

    Purpose

    Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Loren Looger, Michael Davidson

    Article

    Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Type

    Empty backbone

    Tags

    mEos4a

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

35. pCDFDuet-trAtCCD7-OsD27(pYL726 )

    Plasmid

    #178285

    Purpose

    Co-Expresses OsD27 and trAtCCD7 in E. coli. pCDFDuet-1 carrying D27 from Oryza sativa and N-terminus 31 amino-acid truncated CCD7 from Arabidopsis

    Depositor

    Yanran Li

    Article

    Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

    Inserts

    OsD27
    trAtCCD7
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. MSCVhygro-F-G9adSET

    Plasmid

    #41722

    Purpose

    Retroviral expression of human G9a mutant in mammalian cells

    Depositor

    Kai Ge

    Article

    Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

    Insert

    G9adset (EHMT2 Human)
    

    Use

    Retroviral

    Tags

    flag

    Expression

    Mammalian

    Available Since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSG5-FER

    Plasmid

    #30191

    Depositor

    Nora Heisterkamp

    Article

    Hao et al Mol Cell Biol. 1989 Apr . 9(4):1587-93.

    Insert

    Fer tyrosine kinase (FER Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

38. pMaCTag-Z34

    Plasmid

    #120062

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 3xFLAG

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    3xFLAG
    

    Use

    Pcr template

    Tags

    3xFLAG

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMaCTag-P27

    Plasmid

    #120038

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 1xHA

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    1xHA
    

    Use

    Pcr template

    Tags

    1xHA

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRU54

    Plasmid

    #167680

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::spCas9
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge