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Showing: 11051 - 11100 of 79977 results

  7. pISV-DsRed1-nopP

    Plasmid
    #171025
    Purpose
    Agrobacterium rhizogenes-mediated hairy root transformation of Lotus japonicus with nopP, a type 3 effector gene of Sinorhizobium sp. NGR234.
    Depositor
    Christian Staehelin
    Insert
    nopP
    Expression
    Plant
    Promoter
    2 X EN 35S
    Available Since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    171025-plasmid-map-sequence-id-337715

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  19. pANY4-pL18-ccdB

    Plasmid
    #164898
    Purpose
    Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pL18-ccdB.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Tags
    His-tag
    Expression
    Bacterial
    Available Since
    April 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    164898-plasmid-map-sequence-id-327989

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  20. pUPD2 nickase D10A Cas9 (GB1691)

    Plasmid
    #160588
    Purpose
    Nickase D10A Cas9 for Nt fusion.
    Depositor
    Diego Orzaez
    Insert
    nickase D10A Cas9
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160588-plasmid-map-sequence-id-322078

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  21. J-CDAN1A-5

    Plasmid
    #166522
    Purpose
    scFv of a human scaffold targeting Codanin-1. Antigen coverage aa 840-1202 of 1227
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166522-plasmid-map-sequence-id-328268

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  22. Z-OPTN-10

    Plasmid
    #166562
    Purpose
    scFv of a human scaffold targeting Optineurin. Antigen coverage aa 1-510 of 577
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    166562-plasmid-map-sequence-id-327812

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  23. pcDNA-HisFlag-rUSP19ER-CRmut

    Plasmid
    #155253
    Purpose
    for mammalian overexpression of the mutated ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (ER isoform) CR mutant (Usp19 Rat)
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Cys(545) to Arg
    Promoter
    CMV
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155253-plasmid-map-sequence-id-311950

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  24. pEGFP-rUSP19CYT-CAmut

    Plasmid
    #155250
    Purpose
    for mammalian overexpression of the mutated CYT isoform of rat USP19 fused to GFP at N-terminus
    Depositor
    Simon Wing
    Insert
    USP19 (Cytoplasmic isoform) CA mutant (Usp19 Rat)
    Expression
    Mammalian
    Mutation
    Cys(545) to Ala
    Promoter
    CMV
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155250-plasmid-map-sequence-id-311949

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  25. pEGFP-rUSP19ER-CAmut

    Plasmid
    #155249
    Purpose
    for mammalian overexpression of the mutated ER isoform of rat USP19 fused to GFP at N-terminus
    Depositor
    Simon Wing
    Insert
    USP19 (ER isoform) CA mutant (Usp19 Rat)
    Expression
    Mammalian
    Mutation
    Cys(545) to Ala
    Promoter
    CMV
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155249-plasmid-map-sequence-id-311947

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  26. pcDNA-HisFlag-rUSP19ER deltaN3

    Plasmid
    #155243
    Purpose
    for mammalian overexpression of truncated (delta1-1631) ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (delta1-1631) (ER isoform) (Usp19 Rat)
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    155243-plasmid-map-sequence-id-311880

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  27. pcDNA-HisFlag-rUSP19ER deltaN2

    Plasmid
    #155242
    Purpose
    for mammalian overexpression of truncated (delta1-1088) ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (delta1-1088) (ER isoform) (Usp19 Rat)
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    155242-plasmid-map-sequence-id-311878

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  28. pcDNA-HisFlag-rUSP19ER deltaN1

    Plasmid
    #155241
    Purpose
    for mammalian overexpression of truncated (delta1-536) ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (delta1-536) (ER isoform) (Usp19 Rat)
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    155241-plasmid-map-sequence-id-311860

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  29. pcDNA-HisFlag-rUSP19CYT

    Plasmid
    #155238
    Purpose
    for mammalian overexpression of the cytoplasmic isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (Cytoplasmic isoform) (Usp19 Rat, Mouse)
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    155238-plasmid-map-sequence-id-311857

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  30. pCAS9id_TRP

    Plasmid
    #141254
    Purpose
    Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassettes with stuffer and Kpn restriction sites.
    Depositor
    Adam Bertl
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141254-plasmid-map-sequence-id-308894

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  31. pCRII-TOPO CMV-cGFP-SV40 pA + mMALAT1_3'

    Plasmid
    #91803
    Purpose
    Expresses a cGFP mRNA that ends in the MALAT1 triple helix when the upstream SV40 poly(A) signal fails to be used
    Depositor
    Jeremy Wilusz
    Insert
    Coral Green Fluorescent Protein (cGFP)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    91803-plasmid-map-sequence-id-180321

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  32. pSMPUW_8.3_M4_CAMKIIA-gateway-V5-SV40-Zeo

    Plasmid
    #128463
    Purpose
    lentiviral destination vector with CAMKIIA promoter, C-terminal V5 tag, SV40 promoter, and Zeo Resistance gene
    Depositor
    Lindy Barrett
    Insert
    CAMKIIA promoter, C-terminal V5 tag, SV40 promoter, and Zeocin Resistance gene
    Use
    Lentiviral
    Available Since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    128463-plasmid-map-sequence-id-270529

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  33. pX335 Mouse 5' Srcap gRNA B

    Plasmid
    #127905
    Purpose
    Cas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap gene
    Depositor
    Mark Groudine
    Insert
    Srcap gRNA
    Use
    CRISPR
    Available Since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127905-plasmid-map-sequence-id-249688

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  34. pX335 Mouse 5' Srcap gRNA A

    Plasmid
    #127904
    Purpose
    Cas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap gene
    Depositor
    Mark Groudine
    Insert
    Srcap gRNA
    Use
    CRISPR
    Available Since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127904-plasmid-map-sequence-id-249683

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  35. pX330 Mouse 3' Ubf gRNA

    Plasmid
    #127902
    Purpose
    WT Cas9 Vector targeting the 3' end of the mouse Ubf gene
    Depositor
    Mark Groudine
    Insert
    gRNA for Mouse 3' Ubf
    Use
    CRISPR
    Available Since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127902-plasmid-map-sequence-id-249774

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  36. pZDonor-U6-myogenin stop codon gRNA

    Plasmid
    #69549
    Purpose
    Expresses guide RNA targeting near the stop codon of myogenin in the mouse genome.
    Depositor
    Charles Gersbach
    Insert
    Myogenin C terminus-targeting guide RNA
    Use
    CRISPR
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69549-plasmid-map-sequence-id-122972

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  37. CMV-Lin28a-as

    Plasmid
    #120528
    Purpose
    ssRNA expression for dsRNA production
    Depositor
    Petr Svoboda
    Insert
    Lin28 (Lin28a Mouse)
    Use
    RNAi
    Available Since
    Feb. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    120528-plasmid-map-sequence-id-233984

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  39. U6Lin28a-as

    Plasmid
    #120536
    Purpose
    ssRNA expression for dsRNA production
    Depositor
    Petr Svoboda
    Insert
    Lin28 (Lin28a Mouse)
    Use
    RNAi
    Available Since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    120536-plasmid-map-sequence-id-233987

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  40. U6-Lin28a-s

    Plasmid
    #120535
    Purpose
    ssRNA expression for dsRNA production
    Depositor
    Petr Svoboda
    Insert
    Lin28 (Lin28a Mouse)
    Use
    RNAi
    Available Since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    120535-plasmid-map-sequence-id-233980

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  41. CMV-Lin28a-s

    Plasmid
    #120527
    Purpose
    ssRNA expression for dsRNA production
    Depositor
    Petr Svoboda
    Insert
    Lin28 (Lin28a Mouse)
    Use
    RNAi
    Available Since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    120527-plasmid-map-sequence-id-233968

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  42. CMV-Lin28aIR

    Plasmid
    #120524
    Purpose
    dsRNA expression
    Depositor
    Petr Svoboda
    Insert
    Lin28 (Lin28a Mouse)
    Use
    RNAi
    Available Since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    120524-plasmid-map-sequence-id-233961

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  43. pJR288

    Plasmid
    #78550
    Purpose
    For expressing small noncoding RNAs from U6 promoter. One step cloning of oiigonucleotide pairs containing CACC and AAAA overhangs. Ef1a driven mCherry visible marker.
    Depositor
    John Rossi
    Type
    Empty backbone
    Use
    RNAi
    Expression
    Mammalian
    Promoter
    U6, EF1a
    Available Since
    Jan. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    78550-plasmid-map-sequence-id-191096

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  44. pmSod1-Q42L-N’His

    Plasmid
    #117708
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    Sod1 (Sod1 Mouse)
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Q42L
    Promoter
    T7
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117708-plasmid-map-sequence-id-227535

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  45. pmSod1-Q123A-N’His

    Plasmid
    #117709
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    Sod1 (Sod1 Mouse)
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Q123A
    Promoter
    T7
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117709-plasmid-map-sequence-id-227504

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  46. pmSod1-D83G-N’His

    Plasmid
    #117702
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    Sod1 (Sod1 Mouse)
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    D83G
    Promoter
    T7
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117702-plasmid-map-sequence-id-227517

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  47. MoClo adapted pUC57-Amp Level 1 position 7

    Plasmid
    #113044
    Purpose
    Non binary level 1 position 7 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plants
    Depositor
    Elena Caro Bernat
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    Sept. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    113044-plasmid-map-sequence-id-223533

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  48. pHH0103 PQBP1 WW domain

    Plasmid
    #104287
    Purpose
    Bacterial expression of WW domain from PQBP1
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    PQBP1 WW domain (PQBP1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104287-plasmid-map-sequence-id-203724

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  49. pHH0103 HLA-DQA2 WW domain

    Plasmid
    #104297
    Purpose
    Bacterial expression of WW domain from HLA-DQA2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    HLA-DQA2 WW domain (HLA-DQA2 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104297-plasmid-map-sequence-id-203688

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  50. TAP-Amotl2

    Plasmid
    #112834
    Purpose
    transient/stable overexpression
    Depositor
    Tomasz Prószyński
    Insert
    Amotl2 (Amotl2 Mouse)
    Tags
    TAP
    Expression
    Mammalian
    Available Since
    Aug. 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    112834-plasmid-map-sequence-id-217933

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Showing: 11051 - 11100 of 79977 results