We narrowed to 6,994 results for: ef1a
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BII-ChBtW-PLAGL2
Plasmid#133369PurposePiggybac vector for constitutive expressionDepositorInsertPLAGL2 (PLAGL2 Human)
UseTagsFlagExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-BsmBI
Plasmid#162801PurposeSTELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expressionDepositorInsertUb-BsmBI
UsePiggybacTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-MCS-3AID-HA-2A-mTurquoiseBSD
Plasmid#225704PurposeEmpty backbone for lentiviral expression of AID degron tagged POI and mTurquoise fused BlasticidinRDepositorTypeEmpty backboneUseLentiviral and Synthetic BiologyTagsT2A-mTurquoiseExpressionMammalianMutationPromoterpEF1AAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-MCS-3AID-HA-2A-mCherryBSD
Plasmid#225700PurposeEmpty backbone for lentiviral expression of AID degron tagged POI and mCherry fused BlasticidinRDepositorTypeEmpty backboneUseLentiviral and Synthetic BiologyTagsT2A-mCherryExpressionMammalianMutationPromoterpEF1AAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL669
Plasmid#196817PurposeLrCas9 genome editing plasmid in tomatoDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterSlEF1aAvailable sinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
C453-E21: attB4-FerHp>-attB5
Plasmid#162978PurposeGateway attB4/attB5 entry clone for mammalian expression using the human EF1alpha promoterDepositorInserthuman Ferritin heavy chain promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E11: attB4-Grp78p>-attB1r
Plasmid#162970PurposeGateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoterDepositorInserthuman Glucose response protein 78 promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E10: attB4-FerHp>-attB1r
Plasmid#162969PurposeGateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoterDepositorInserthuman Ferritin heavy chain promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4xh7SKPylT_EF1_BsmBI_(C1A)-Intein_DD
Plasmid#162811PurposeControl STELLA destination plasmid for C-terminal labelled proteins. Contains restriction sites for golden gate cloning.DepositorInsertBsmBI_(C1A)-Intein_DD
UsePiggybacTagsExpressionMammalianMutationIntein(Cys1,Ala)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_M
Plasmid#162815PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_MDepositorInsertUb-(N-TAG)CoV2_M
UsePiggybacTagsExpressionMammalianMutationCoV2_M(Met1,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_9b
Plasmid#162816PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_9bDepositorInsertUb-(N-TAG)CoV2_9b
UsePiggybacTagsExpressionMammalianMutationCoV2_9b(Met1,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF6
Plasmid#162817PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF6DepositorInsertUb-(N-TAG)CoV2_ORF6
UsePiggybacTagsExpressionMammalianMutationCoV2_ORF6(Met1,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF7b
Plasmid#162818PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF7bDepositorInsertUb-(N-TAG)CoV2_ORF7b
UsePiggybacTagsExpressionMammalianMutationCoV2_ORF7b(Met1,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF10
Plasmid#162819PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF10DepositorInsertUb-(N-TAG)CoV2_ORF10
UsePiggybacTagsExpressionMammalianMutationCoV2_ORF10(Met1,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF9c/14
Plasmid#162820PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF9c/14DepositorInsertUb-(N-TAG)CoV2_ORF9c/14
UsePiggybacTagsExpressionMammalianMutationCoV2_ORF9c/14(Met1,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b_alt
Plasmid#162821PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b_altDepositorInsertUb-(N-TAG)CoV2_ORF3b_alt
UsePiggybacTagsExpressionMammalianMutationCoV2_ORF3b_alt(Met1,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b(CoV1h)
Plasmid#162822PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b(CoV1h)DepositorInsertUb-(N-TAG)CoV2_ORF3b(CoV1h)
UsePiggybacTagsExpressionMammalianMutationCoV2_ORF3b(CoV1h)(Met1,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA_IRES_BSD
Plasmid#162805PurposeSTELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integrationDepositorInsertUb-BsmBI-HA_IRES_Blasticidin
UsePiggybacTagsHAExpressionMammalianMutationPromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-PIR702W-Luc
Plasmid#133362PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertIRFP-702
UseTagsHA-tagged IRFPExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-PIR702W
Plasmid#133361PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertIRFP-702
UseTagsHA-tagged IRFPExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-PnGW-Luc
Plasmid#133360PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertGFP-NLS
UseTagsHA-tagged GFPExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB410
Plasmid#68675PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTags3xFLAGExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB413
Plasmid#68678PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsGlucocorticoid receptor (GR)ExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB210
Plasmid#68601PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTags3xFLAGExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
alpha7nACHR_Nacho_GCAMP7s
Plasmid#182940Purposealpha7 nACHR expression in mammalian cell lines with calcium reporterDepositorInsertNACH7 (CHRNA7 Human)
UseTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2-MG-IRES2-mNeonGreen
Plasmid#175508PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2-MG-IRES2-mTagBFP2
Plasmid#175509PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mTagBFP2 (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-cilia-APEX
Plasmid#73186PurposeExpresses cilia-APEX in mammalian cellsDepositorInsertcilia-APEX (Nphp3 Glycine max, Mouse)
UseTagsinternal GFP tagExpressionMammalianMutationPromoterEF1alphaAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-FHC-POLQ
Plasmid#64875Purposelentiviral expression of human POLQDepositorInsertPOLQ (POLQ Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationPromoterEF1alphaAvailable sinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only