We narrowed to 9,202 results for: sacs

1. pWN021

   Plasmid

   #233851

   Purpose

   MoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NLS-NS3-V2-VP48-2XOaf1-tADH1

   Depositor

   Jeff Hasty

   Article

   O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

   Insert

   pTDH3-LexA-NLS-NS3-V2-VP48-2XOaf1-tADH1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWN081

   Plasmid

   #233858

   Purpose

   MoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NES-NS3a-VP48-2XOaf1-tADH1

   Depositor

   Jeff Hasty

   Article

   O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

   Insert

   pTDH3-LexA-NES-NS3a-NLS-VP48-2XOaf1-tADH1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pWN103

   Plasmid

   #233860

   Purpose

   MoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NES-NS3-V1-NLS-VP48-2XOaf1-tADH1

   Depositor

   Jeff Hasty

   Article

   O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

   Insert

   pTDH3-LexA-NES-NS3-V1-NLS-VP48-2XOaf1-tADH1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pWN104

   Plasmid

   #233861

   Purpose

   MoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NES-NS3-V2-NLS-VP48-2XOaf1-tADH1

   Depositor

   Jeff Hasty

   Article

   O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

   Insert

   pTDH3-LexA-NES-NS3-V2-NLS-VP48-2XOaf1-tADH1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAL004

   Plasmid

   #233863

   Purpose

   MoClo-YTK URA multigene plasmid with MCS; Tet systems; pRNR2-rtTA-v16-tADH1; tetO7-pLEU2m-MCS-tENO2

   Depositor

   Jeff Hasty

   Article

   O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

   Insert

   pRNR2-rtTA-v16-tADH1; tetO7-pLEU2m-MCS-tENO2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAL003

   Plasmid

   #233864

   Purpose

   MoClo-YTK URA multigene plasmid with MCS; Tet systems; pRPL18B-rtTA3-tADH1; tetO7-pLEU2m-MCS-tENO2

   Depositor

   Jeff Hasty

   Article

   O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

   Insert

   pRPL18B-rtTA3-tADH1; tetO7-pLEU2m-MCS-tENO2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYO734

   Plasmid

   #235753

   Purpose

   Protein expression of ScVPS30 and ZZ-ScVPS38

   Depositor

   Roger Williams

   Article

   Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

   Inserts

   VPS30 (VPS30 Budding Yeast)
   VPS38 (VPS38 Budding Yeast)
   

   Use

   Tags

   ZZ-3xTEV

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pYO761

   Plasmid

   #235760

   Purpose

   Expression of ScVPS38 delta BARA2 (320-440)-x3FLAG under its own promoter

   Depositor

   Roger Williams

   Article

   Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

   Insert

   VSP38 delta BARA2
   

   Use

   Tags

   3xFLAG

   Expression

   Yeast

   Mutation

   aa 320-440 deleted

   Promoter

   ScVPS38 promoter

   Available since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYO766

   Plasmid

   #235763

   Purpose

   Expression of ScVPS38 delta BARA2 (320-440)-EGFP under its own promoter

   Depositor

   Roger Williams

   Article

   Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

   Insert

   VSP38 delta BARA2
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   aa 320-440 deleted

   Promoter

   ScVPS38 promoter

   Available since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYO933

    Plasmid

    #235765

    Purpose

    Expression of ScVPS34 delta C154-V202-3xFLAG under its own promoter

    Depositor

    Roger Williams

    Article

    Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

    Insert

    VPS34 delta C154-V202
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    aa 154-202 deleted

    Promoter

    ScVPS34 promoter

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFA6-CFAPoptim-NAT

    Plasmid

    #221129

    Purpose

    Plasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::NATMX

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRS413-TEF1pr-SEC61-FAPoptim

    Plasmid

    #221126

    Purpose

    FAP-tagged marker for the ER (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    SEC61-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCfB2188-PrARG8-GFP::URA3

    Plasmid

    #216512

    Purpose

    Integrative fluorescent reporter for amino acid biosynthesis sensing pathways, comprising GFP driven by the ARG8 promoter within the Easy clone vector, requires NotI digestion for integration in yeast

    Depositor

    Juha Saarikangas

    Article

    Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.

    Insert

    PrARG8-yeGFP
    

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ARG8

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCfB2197-PrGNP1-mK2-NatMX

    Plasmid

    #216513

    Purpose

    Integrative fluorescent reporter for amino acid uptake sensing pathway, comprising mKate2 driven by the GNP1 promoter within the Easy clone vector, requires NotI digestion for integration in yeast

    Depositor

    Juha Saarikangas

    Article

    Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.

    Insert

    PrGnp1-mKate2
    

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GNP1

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRS415-TEF1pr-STE3-GFP

    Plasmid

    #221113

    Purpose

    GFP-tagged STE3 (C-terminally tagged, optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    STE3-GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAG415Gal_Gcn4 SATtoG KtoRHA

    Plasmid

    #232994

    Purpose

    Galactose iduced expression of Gcn4 SATtoG KtoRHAin yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 SATtoG KtoR
    

    Use

    Tags

    3xHA and TEV cleavage site

    Expression

    Yeast

    Mutation

    S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAG415Gal_Gcn4 SATtoG KtoR

    Plasmid

    #232961

    Purpose

    Galactose iduced expression of Gcn4 ATtoG KtoR in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 SATtoG KtoR
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAG415Gal_Gcn4 ILVtoED solvvol

    Plasmid

    #232958

    Purpose

    Galactose iduced expression of Gcn4 LVtoED solvvol in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 ILVtoED solvvol
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAG415Gal_Gcn4 SATtoG KtoRGFP

    Plasmid

    #233006

    Purpose

    Galactose iduced expression of Gcn4 SATtoG KtoRGFP in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 SATtoG KtoR
    

    Use

    Tags

    HA-GFP-HA and TEV cleavage site

    Expression

    Yeast

    Mutation

    S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEW107

    Plasmid

    #232796

    Purpose

    COMPASS Fragment 2 (His-FLAG-ySet1, Cps30, Twinstrep-Cps40) in pBIG2ab

    Depositor

    Cynthia Wolberger

    Article

    Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1

    Insert

    COMPASS Fragment 2 (His-FLAG-ySet1, Cps30, Twinstrep-Cps40) (SET1, SPP1, SWD3 Budding Yeast)
    

    Use

    Tags

    His6-3xFLAG, Twinstrep

    Expression

    Insect

    Mutation

    ySet1(762-1080)

    Promoter

    polyhedrin promoter

    Available since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. pXTK121 [Misc_CEN6-ARS4-HIS3_PartType11]

    Plasmid

    #229358

    Purpose

    XanthoMoClo Golden Gate Part Plasmid for cloning, contains Misc_CEN6-ARS4-HIS3_PartType11

    Depositor

    Michael Springer

    Article

    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

    Insert

    CEN6-ARS4-HIS3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFA6a-FLAG-APEX2-NES-HIS3

    Plasmid

    #229230

    Purpose

    Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

    Depositor

    Fulvio Reggiori

    Article

    Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRS413-TEF1pr-RPA34-FAPoptim

    Plasmid

    #221123

    Purpose

    FAP-tagged marker for the nucleus (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    RPA34-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDest17_Gcn4 ILVtoED solvvol

    Plasmid

    #231858

    Purpose

    Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED solvvol

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 ILVtoED solvvol
    

    Use

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

    Promoter

    T7

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFA6a-FLAG-APEX2-NES-K.l.LEU2

    Plasmid

    #229229

    Purpose

    Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

    Depositor

    Fulvio Reggiori

    Article

    Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFA6a-FLAG-APEX2-NES-NatMX6

    Plasmid

    #229231

    Purpose

    Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

    Depositor

    Fulvio Reggiori

    Article

    Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFA6a-FLAG-APEX2-NES-TRP1

    Plasmid

    #229228

    Purpose

    Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

    Depositor

    Fulvio Reggiori

    Article

    Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLANT-2b

    Plasmid

    #231487

    Purpose

    Expresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.

    Depositor

    Venkat Gopalan

    Inserts

    SUMO^NIT (SMT3 Budding Yeast)
    FraB
    

    Use

    Tags

    His6 tag and His6-SUMO^NIT

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-6 (MEPEES). and M83L, E84Q,…

    Promoter

    T7 promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCas9-EcRT-Z3-Kan

    Plasmid

    #232096

    Purpose

    Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    Z3 promoter and Z3EV transcription factor
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Z3

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. YIplac128-TEF2p-LDH-CYC1t

    Plasmid

    #216120

    Purpose

    Yeast integrative vector for constitutive expression of LDH at the LEU2 locus

    Depositor

    Yvonne Nygård

    Article

    Choi et al Metab Eng. 2024 Jul;84:23-33. doi: 10.10

    Insert

    Lactate dehydrogenase (LDH Bovine)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF2 promoter

    Available since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge