We narrowed to 10,599 results for: cat.1

1. pTDpelB-CTwinStrep

   Plasmid

   #45941

   Purpose

   use to create N-terminal PelB and C-terminal Twin-Strep-tagged fusion proteins for periplasmic translocation via PelB signal sequence

   Depositor

   Thorben Dammeyer

   Article

   Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

   Type

   Empty backbone

   Use

   Tags

   PelB and Twin-Strep

   Expression

   Bacterial

   Mutation

   Promoter

   Trc (lactose/IPTG inducible)

   Available since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAGEN-SBP-DICER1

   Plasmid

   #50558

   Purpose

   purification of Dicer1 from mammalian cells

   Depositor

   Yukihide Tomari

   Article

   Betancur et al RNA. 2012 Jan;18(1):24-30. doi: 10.1261/

   Insert

   Dicer1 (Dicer1 Mouse)
   

   Use

   Tags

   SBP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pHPK1212

   Plasmid

   #8834

   Depositor

   David Waugh

   Article

   Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

   Insert

   tobacco vein mottling virus protease, catalytic domain
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a-SnoopTag-SpyTag-(AffiHER2)x3

   Plasmid

   #216281

   Purpose

   Three affibodies to HER2 linked in a chain for bacterial expression. SnoopTag and SpyTag allow isopeptide bond formation to their cognate Catcher

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   SnoopTag-SpyTag-(AffiHER2)x3
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6, SnoopTag, and SpyTag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pETM11-6xHis-TEV-hORF1p_R261A

   Plasmid

   #202586

   Purpose

   Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the R261A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   ORF1p
   

   Use

   Tags

   6x His and TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   Changed ORF1p Arginine 261 to Alanine

   Promoter

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLT237

   Plasmid

   #174300

   Purpose

   Replicating plasmid with homology region for introducing PolCC669Y mutation. Confers thiamphenicol resistance.

   Depositor

   Lee Lynd

   Article

   Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

   Insert

   PolC homology arm
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changed Cysteine 669 to Tyrosine

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. T7-TetO-GFP_ColE1

   Plasmid

   #173193

   Purpose

   Expresses GFP under the control of a T7-TetO promoter. Expression can be repressed via TetR, and repression can be relieved via addition of tetracycline. Contains the ColE1 origin of replication.

   Depositor

   Tara Deans

   Article

   MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

   Insert

   T7-TetO
   

   Use

   Tags

   ssrA degradation tag (DAS+4)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJH858

   Plasmid

   #172176

   Purpose

   PylB overexpression and purification

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   PT7/Lac-[His6-TEV-SUMO-PylB.3f2deopt]
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJH859

   Plasmid

   #172177

   Purpose

   PylB overexpression and purification

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   PT7/Lac-[His6-TEV-SUMO-PylB.JM10.1deopt]
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJH768v2

    Plasmid

    #172173

    Purpose

    PylB overexpression and purification

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    PT7/Lac-[His6-TEV-SUMO-PylB.3f2]
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB(N61S, E84…

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJH769v2

    Plasmid

    #172174

    Purpose

    PylB overexpression and purification

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    PT7/Lac-[His6-TEV-SUMO-PylB.JM10.1]
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB(N61S, E84…

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAV-Synapsin-JNKKTR -miRFP670-WPRE

    Plasmid

    #159976

    Purpose

    single wavelength translocating reporter of the JNK pathway

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    JNKKTR -miRFP670
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. A102-GAP-BoCCS

    Plasmid

    #123287

    Purpose

    Expression of truncated Glo3 (1-375) in E.Coli

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    Glo3 (GLO3 Budding Yeast)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    1-375

    Promoter

    T5

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. YAP1-V5 in pLX304

    Plasmid

    #42555

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR221_SLC33A1

    Plasmid

    #132264

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC33A1 (SLC33A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. FCM101

    Plasmid

    #194298

    Purpose

    E. coli expression vector for monobody adaptor, FCM101, that bind to the Fc region of mouse IgG1 subclass. It contains a single Cys residue at the C-terminus for chemical conjugation, N-terminal His6

    Depositor

    Shohei Koide

    Article

    Albert et al Nat Commun. 2022 Oct 11;13(1):5998. doi:

    Insert

    FCM101
    

    Use

    Tags

    His tag, FLAG tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. shCTNNB1 # 2

    Plasmid

    #42544

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    CTNNB1 (CTNNB1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. shBCL2L1 # 2

    Plasmid

    #42552

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    shBCL2L1 (BCL2L1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pCCL-PGK-SPdCas9-BFP-DNMT3A

    Plasmid

    #66819

    Purpose

    dCas9 fused to BFP and the human DNMT3A catalytic domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-BFP-DNMT3A, catalytic domain of DNMT3A
    

    Use

    Tags

    dSpCas9-BFP-DNMT3A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCCL-PGK-SPdCas9-BFP-DNMT3B

    Plasmid

    #66820

    Purpose

    dCas9 fused to BFP and the human DNMT3B catalytic domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-BFP-DNMT3B, catalytic domain of DNMT3B
    

    Use

    Tags

    dSpCas9-BFP-DNMT3B

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge