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pAc5.1B-EGFP-DmBam_AA

Plasmid

#148287

Purpose

Insect Expression of DmBam

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam (bam Fly)

Expression

Insect

Mutation

one non silent mutation A239 to S, described as …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmBam_AA

Plasmid

#148289

Purpose

Insect Expression of DmBam

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam (bam Fly)

Expression

Insect

Mutation

one non silent mutation A239 to S, described as …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-HsNot7_T

Plasmid

#147637

Purpose

Bacterial Expression of HsNot7

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

HsNot7 (CNOT7 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpH-HsNot10_25-707_Q

Plasmid

#147343

Purpose

Bacterial Expression of HsNot10_25-707

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

HsNot10_25-707 (CNOT10 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1-TSF-ε

Plasmid

#196583

Purpose

Expresses the two Strep/one FLAG (TSF)-tagged human AP-4 ε subunit in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

Insert

AP-4 ε subunit (AP4E1 Human)

Tags

two Strep/one FLAG (TSF)

Expression

Mammalian

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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Sandgrouse+R111Q–insD

Plasmid

#191229

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

removed aspartic acid at 121 and replaced arginin…

Promoter

PH and p10

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-MS2-HA-C1-DmBam_AG

Plasmid

#148837

Purpose

Mammalian Expression of DmBam

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam (bam Fly)

Expression

Mammalian

Mutation

one non silent mutation A239 to S, described as …

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-MS2-HA-C1-DmBam-L17EM24EL28EV32E_AG

Plasmid

#148838

Purpose

Mammalian Expression of DmBam-L17EM24EL28EV32E

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam-L17EM24EL28EV32E (bam Fly)

Expression

Mammalian

Mutation

one non silent mutation A239 to S, described as …

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-DmBam_AG

Plasmid

#148840

Purpose

Mammalian Expression of DmBam

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam (bam Fly)

Expression

Mammalian

Mutation

one non silent mutation A239 to S, described as …

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pPhiC31-actIIORF4

Plasmid

#190785

Purpose

The PhiC31-phage based integration vector containing the actII-ORF4 gene under the control of strong kasOp* promoter

Depositor

Jan Kormanec

Article

Novakova et al Appl Microbiol Biotechnol. 2022 Nov;106(

Inserts

T5 terminator
actII-4
kasOp*

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG-HUWE1_YH/ GG_delta3291-3298

Plasmid

#187127

Purpose

mammalian cell expression of FLAG-HUWE1 Y355G/H356G, delta3291-3298

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Y355G/H356G, delta3291-3298

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG-HUWE1_delta169-189

Plasmid

#187135

Purpose

mammalian cell expression of 3xFLAG-HUWE1 delta169-189

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

delta169-189

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG-HUWE1_delta3896-3951

Plasmid

#187133

Purpose

mammalian cell expression of 3xFLAG-HUWE1 delta3896-3951

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

delta3896-3951

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG-HUWE1_I3969A/F3982A

Plasmid

#187129

Purpose

mammalian cell expression of 3xFLAG-HUWE1 I3969A/F3982A

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

I3969A/F3982A

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_UBA/UIM/UBM-mut

Plasmid

#187153

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

L2976A/P2977A/M1328A/F1330A/A1376G/S1382A

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pST_DHN_TaDHN5 (pBS0801)

Plasmid

#185262

Purpose

For the mammalian expression of the durm wheat protein DHN_TaDHN5. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DHN_TaDHN5

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_DHN_OsLEA5 (pBS0793)

Plasmid

#185259

Purpose

For the mammalian expression of the rice plant protein DHN_OsLEA5. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DHN_OsLEA5

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pnYC-NvM-DmBam_141-442_AG

Plasmid

#148833

Purpose

Bacterial Expression of DmBam_141-422

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam_141-422 (bam Fly)

Expression

Bacterial

Mutation

one non silent mutation A239 to S, described as …

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-His10-BTLAicd (AA190-289)-TwinStrep

Plasmid

#180805

Purpose

Expression of 10*His tagged human BTLA intracellular domain (AA190-289)-TwinStrep

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

BTLAint (AA190-289)-TwinStrep (BTLA Human)

Expression

Bacterial

Promoter

T7

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-6p-2-GST-PD-1icd (AA194-288)

Plasmid

#180814

Purpose

Expression of GST fused human PD-1 intracellular domain (AA194-288)

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

PD-1int (AA194-288) (PDCD1 Human)

Expression

Bacterial

Promoter

Tac

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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