We narrowed to 9,933 results for: Uty
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Plasmid#213709PurposeTo express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.DepositorInsertEGFP
UseCRISPRTags3XFLAG and Twin-strep-tagExpressionMammalianMutationPromoterCMVAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-F14F15S-jGCaMP7s
Plasmid#178514PurposeAAV vector for Flpo recombinase dependent jGCaMP7sDepositorInsertjGCaMP7s
UseAAVTagsExpressionMammalianMutationPromoterAvailable SinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lnig-INTR-GFP
Plasmid#208904Purposeencodes the inotocin receptor (oxytocin/vasopressin-like) from the ant Lasius niger, tagged with GFPDepositorInsertINTR
UseTagsEGFPExpressionMammalianMutationPromoterAvailable SinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVTHM_mePOU_3xFlag
Plasmid#206395PurposeExpresses 3xflag fused mouse ePOU in mammalian cells, for lentivirus generation.DepositorInsertePOU
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterAvailable SinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
186_pETcon_SARS2_XBB1-5
Plasmid#208086Purposeyeast surface display of the SARS-CoV-2 XBB.1.5 variant RBDDepositorInsertSARS-CoV-2 XBB.1.5 RBD (S Budding Yeast, SARS-CoV-2 virus)
UseTagsHA and c-MycExpressionYeastMutationPromoterAvailable SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pminiTol2-4kb wnt3 promoter-Fzd1mApple
Plasmid#203795PurposeTol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of Fzd1-mApple for zebrafish transgenesisDepositorInsertFzd1 (fzd1 Zebrafish)
UseZebrafish expressionTagsmAppleExpressionMutationPromoter4kb Wnt3 promoterAvailable SinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24_T24_pRham-ilvA-relE_RBS3_CB2
Plasmid#201533PurposeExpressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorInsertilvA-relE_RBS3_CB2
UseSynthetic BiologyTagsExpressionMutationrhaR L128+1bp (frameshift), ilvA G455CPromoterPrhaBAD (rhamnose)Available SinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24_T24_pRham-ilvA-relE_RBS3_CB4
Plasmid#201535PurposeExpressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorInsertilvA-relE_RBS3_CB4
UseSynthetic BiologyTagsExpressionMutationrhaS ΔM1-H5, PrhaSR Δ179bpPromoterPrhaBAD (rhamnose)Available SinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRANS-Pho2-1/2-2-Csy4
Plasmid#161763PurposepTRANS T-DNA vector with nptII selection and 35S:Cas9 with 6x Csy4-spliced gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113)DepositorInsertCas9 and gRNAs
UseTagsExpressionPlantMutationPromoterCmYLCV PromoterAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRANS-Pho2-1/2-2-tRNA
Plasmid#161762PurposepTRANS T-DNA vector with nptII selection and 35S:Cas9 with 6x tRNA-spliced gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113)DepositorInsertCas9 and gRNAs
UseTagsExpressionPlantMutationPromoterCmYLCV PromoterAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-ZNF354C_mutant_M41K-L42K-E43K
Plasmid#194185PurposeExpresses ZNF354C with mutated KRAB domain (M41K, L42K, E43K mutation)DepositorInsertZNF354C (ZNF354C Human)
UseTagsMyc-DDKExpressionMammalianMutationKRAB domain mutant M41K/L42K/E43KPromoterCMVAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
iEscSnFR
Plasmid#182807PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiEscSnFR
UseTags6xHis, HA and MycExpressionBacterialMutationPromoterT7Available SinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
iFluoxSnFR
Plasmid#182808PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiFluoxSnFR
UseTags6xHis, HA and MycExpressionBacterialMutationPromoterT7Available SinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.6RT4-Pct5.1-crRNA(hcdR)-RT(ΔhcdR)
Plasmid#191646PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(hcdR-targeting spacer) hcdR-deleting repair template, used for hcdR deletionDepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Chinchilla–insM
Plasmid#191226PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationremoved methionine at 122 in the protein sequencePromoterPH and p10Available SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Chinchilla+N122D–insM
Plasmid#191227PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationremoved methionine at 122 and replaced asparagine…PromoterPH and p10Available SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sandgrouse–insD
Plasmid#191228PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationremoved aspartic acid at 121 in the protein seque…PromoterPH and p10Available SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjT-tdTomato-PGK-Neo
Plasmid#186173PurposeKnock-in gene targeting in marmoset cells at TBXT locusDepositorInserttdTomato
UseMarmoset targetingTagsExpressionMutationPromoterAvailable SinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjOSR1-EGFP-PGK-Puro
Plasmid#186170PurposeKnock-in gene targeting in marmoset cells at OSR1 locusDepositorInsertEGFP
UseMarmoset targetingTagsExpressionMutationPromoterAvailable SinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjSF1-tdTomato-PGK-Neo
Plasmid#186172PurposeKnock-in gene targeting in marmoset cells at SF1 locusDepositorInserttdTomato
UseMarmoset targetingTagsExpressionMutationPromoterAvailable SinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only