We narrowed to 39,261 results for: lat
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Plasmid#172611PurposeRetroviral vector for the constitutive expression of FLAG-2xSTREP-tagged p27 and a puromycin resistance marker in mammalian cellsDepositorInsertCDKN1B (CDKN1B Human)
UseRetroviralTagsFLAG-2xSTREPExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSF-DUET1-DHDDS/Sumo-NgBR
Plasmid#203162PurposeExpression of human cis-prenyltransferase complex for purificationDepositorUseTagsHis6-SUMOExpressionBacterialMutationtruncationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Venus1-WTSTAT3
Plasmid#123164PurposeExpresses wild type STAT3 fused to the 1-157 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
UseTagsVenus1 (aa 1-158) for bimolecular fluorescence co…ExpressionMammalianMutationnonePromoterpCMVAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MKK1K97A
Plasmid#107974Purposeexpression of a dominant negative MAP Kinase KinaseDepositorInsertMKK1 K97A (MAP2K1 Human)
UseTagsExpressionMammalianMutationdominant negative; see Depositor Comments belowPromoterCMVAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR207 SARS-CoV-2 NSP1 Delta RC
Plasmid#164522PurposeGateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions K164A/H165A)DepositorInsertNSP1 Delta RC (ORF1ab )
UseTagsExpressionMammalianMutationInsert of NSP1 gene's CDS from SARS-CoV-2 is…PromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-KDM2B-P2A-mCherry
Plasmid#203852PurposeTet-inducible lentiviral plasmid expressing KDM2B-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertKDM2B (KDM2B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIRESh-GFPII-MB-Dlk1
Plasmid#108930PurposeGFP-tagged vector expressing membrane-bound Dlk1DepositorInsertDlk1 (Dlk1 Mouse)
UseTagsGFPIIExpressionMammalianMutationlacks coding sequence for the juxtamembrane motif…PromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-PuroR_BACH2-sgRNA8
Plasmid#71828PurposeExpression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a controlDepositorInsertBACH2-sgRNA8 (BACH2 S. pyogenes, Human, Synthetic)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-PuroRExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterU6Available sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-pGC-A
Plasmid#186626PurposepFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)DepositorInsertNPR1 (NPR1 Human)
UseTagshemagglutinin (HA) signal peptide + His10-tag + T…ExpressionInsectMutationdeleted amino acids 1-32PromoterpolyhedringAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-FOSL2-P2A-mCherry
Plasmid#203860PurposeTet-inducible lentiviral plasmid expressing FOSL2-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertFOSL2 (FOSL2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Htt171-18Q-myc-WPRE
Plasmid#107936PurposeAdeno-Associated viral (AAV) vector plasmid expressing exon1 of wild type HTT (wtHTT, 18 polyQ repeats) in neuronsDepositorInsertExon 1 of wild type HTT (HTT Human)
UseAAVTagsExpressionMutationPromoterhSynapsinAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
Venus2-WTSTAT3
Plasmid#123165PurposeExpresses wild type STAT3 fused to the 158-238 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
UseTagsVenus2 (aa 158-238) for bimolecular fluorescence …ExpressionMammalianMutationnonePromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
SARS CoV-2 HexaPro spike (bio-His)
Plasmid#166856PurposeHuman expression vector encoding full length SARS CoV-2 spike protein, containing biotinylation site and 8His-tag. Modification of Addgene plasmid #154754.DepositorInsertSARS CoV-2 HexaPro full length spike protein (S Severe acute respiratory syndrome-related coronavirus)
UseTags8xHis and Biotinylation siteExpressionMammalianMutationPromoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mNeon-PuroR [M1G]
Plasmid#171800PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and puromycin resistance cassette [p.M1G]DepositorInsertmNeonGreen-T2A-PuroR
UseGene taggingTagsExpressionMutationPuroR: Changed Methionin 1 to Glycine.PromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIRESh-GFPII-S-Dlk1
Plasmid#108931PurposeGFP-tagged vector expressing secreted isoform of Dlk1DepositorInsertDlk1 (Dlk1 Mouse)
UseTagsGFPIIExpressionMammalianMutationcontains sequences from full length Dlk1A up to t…PromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28-Dnmt3a3L-sc27
Plasmid#71827PurposeFor bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.DepositorInsertDnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)
UseTags6xHis tagExpressionBacterialMutationPromoterAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV-BRAF-REG-Halo
Plasmid#194771PurposeMammalian expression construct encoding the BRAF regulatory (REG) domain (AA 1-435) with a C-terminal HaloTag.DepositorInsertBRAF regulatory (REG) domain (AA 1-435) (BRAF Human)
UseTagsHaloTagExpressionMammalianMutationPromoterCMV51Available sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-mFgf21pro2-luc
Plasmid#101798PurposeReporter for mouse Fgf21 promoter activityDepositorInsertFibroblast growth factor 21 promoter (Fgf21 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
Venus2-Y705F/S727A-STAT3
Plasmid#123177PurposeExpresses Y705F/S727A STAT3 double mutant fused to the 158-238 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
UseTagsVenus2 (aa 158-238) for bimolecular fluorescence …ExpressionMammalianMutationDouble Y705F and S727A substitutionsPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mNeon-BlastR [M1G]
Plasmid#171805PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and blasticidin resistance cassette [p.M1G]DepositorInsertmNeonGreen-T2A-BlastR
UseGene taggingTagsExpressionMutationBlastR: Changed Methionin 1 to Glycine.PromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only