We narrowed to 24,466 results for: nes

1. pAAV-FLEXfrt-SaCas9-U6-sgGabrg3

   Plasmid

   #124872

   Purpose

   Mutagenesis of Gabrg3

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gabrg3 (Gabrg3 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-FLEXfrt-SaCas9-U6-sgGabrg2

   Plasmid

   #124871

   Purpose

   Mutagenesis of Gabrg2

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gabrg2 (Gabrg2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJSC272 - Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant

   Plasmid

   #101236

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant K918A/V922A/R925A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   K918A, V922A, R925A and Q926A

   Promoter

   T7

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. TET-pLKO.1 PURO shId2 #1

   Plasmid

   #83088

   Purpose

   Lentiviral shRNA vector for inducible knockdown of mouse Id2

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   shId2 (Id2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

5. pXL-BacII-CAG-Zeo-t2A-sHRPaNLG1

   Plasmid

   #79914

   Purpose

   piggyBac transposon vector, Zeocin-2A-sHRPbNLG1

   Depositor

   Joshua Sanes

   Article

   Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   Zeocin-t2A-splitHRP-aNLG1 (Nlgn1 Mouse)
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. exon L-firefly

   Plasmid

   #81006

   Purpose

   luciferase based mini-gene to report splicing at exon 25 in Drosophila parayltic

   Depositor

   Richard Baines

   Article

   Lin et al Brain. 2015 Apr;138(Pt 4):891-901. doi:

   Insert

   paralytic (para Fly)
   

   Use

   Tags

   firefly luciferase

   Expression

   Insect

   Mutation

   spanning exon 24 to exon 26, termination codon in…

   Promoter

   P-AC5 (Actin 5C)

   Available since

   Aug. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-SENP2 WT

   Plasmid

   #126591

   Purpose

   Expresses siRNA resistant SENP2 (WT) in mammalian cells, Dox inducible in TetR cell lines

   Depositor

   Joanna Morris

   Article

   Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

   Insert

   SENP2 (SENP2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   siRNA resistant to GAGGAGAUAUUCAGACAUU

   Promoter

   CMV

   Available since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-C1-MKLP1 S708A

   Plasmid

   #70146

   Purpose

   mammalian expression of MKLP1 with an eGFP fusion and an S708A mutation

   Depositor

   Masanori Mishima

   Article

   Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

   Insert

   MKLP1 (KIF23 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   S708A

   Promoter

   CMV

   Available since

   Nov. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5-FRT/TO-neo-C11orf51-3xFlag (2765)

   Plasmid

   #39859

   Depositor

   Jonathon Pines

   Article

   Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

   Insert

   C11orf51 (ANAPC15 Human)
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA5/FRT/TO_TAP_FAM86A

    Plasmid

    #85116

    Purpose

    mammalian expression of FAM86A

    Depositor

    Pal Falnes

    Article

    Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

    Insert

    EEF2KMT (FAM86A Human)
    

    Use

    Tags

    CBP-SBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDRF'- AmTryoshka1;3-LS-F138I

    Plasmid

    #100922

    Purpose

    Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    AmTryoshka1;3-LS-F138I (AMT1;3 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    F138I

    Promoter

    PMA

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDRF'- AmTryoshka1;3-LS-L255I

    Plasmid

    #100964

    Purpose

    Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    AmTryoshka1;3-LS-L255I (AMT1;3 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    L255I

    Promoter

    PMA

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDRF'- AmTryoshka1;3-GS-F138I

    Plasmid

    #100965

    Purpose

    Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    AmTryoshka1;3-GS-F138I (AMT1;3 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    F138I

    Promoter

    PMA

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

14. pDRF'- AmTryoshka1;3-GS-L255I

    Plasmid

    #100966

    Purpose

    Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    AmTryoshka1;3-GS-L255I (AMT1;3 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    L255I

    Promoter

    PMA

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFP-C1-MKLP1 S710A

    Plasmid

    #70149

    Purpose

    mammalian expression of MKLP1 with an eGFP fusion and an S710A mutation

    Depositor

    Masanori Mishima

    Article

    Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

    Insert

    MKLP1 (KIF23 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S710A

    Promoter

    CMV

    Available since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJSC236 - Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant

    Plasmid

    #101235

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant F491A/M495A/T496A/N497A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    F491A, M495A, T496A, N497A and Q926A

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJSC239 - Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant

    Plasmid

    #101234

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant T657A/G658A/W659A/R661A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    T657A, G658A, W659A, R661A and Q926A

    Promoter

    T7

    Available since

    Nov. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJSC269 - Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant

    Plasmid

    #101220

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant N692A/M694A/Q695A/H698A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A, H698A and Q926A

    Promoter

    T7

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJSC197 - Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant

    Plasmid

    #101232

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant G528A/V583A/E584A/D585A/N588A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    G528A, V583A, E584A, D585A, N588A and Q926A

    Promoter

    T7

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. tet-pLKO shMKP7.v1 puro

    Plasmid

    #87794

    Purpose

    Expresses an inducible short hairpin targeting human MKP7 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shMKP7 (DUSP16 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE tight

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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