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pcDNA5/FRT/TO_STf

Plasmid

#182000

Purpose

CMV overexpression of SNAP-tag fast in mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

STf

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_STf_SKL

Plasmid

#182007

Purpose

CMV overexpression of SNAP-tag fast in the peroxisome of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

STf_SKL

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pSLS.601

Plasmid

#184955

Purpose

Express -Eco1 ncRNA library variants

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 ncRNA (variants)

Expression

Bacterial

Mutation

Library members with mutations described in manus…

Promoter

T7/lac

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB (TIR23)-sfGFP

Plasmid

#189676

Purpose

titratable expression of sfGFP in bacteria

Depositor

Daniel Daley

Article

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

sfGFP

Tags

His-T7-EK

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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LDB221

Plasmid

#185427

Purpose

Bacterial expression of Nup1 C-terminal residues 777-1076 as GST fusion

Depositor

Ken Belanger, Laura Davis

Article

Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

Insert

NUP1

Tags

GST

Expression

Bacterial

Mutation

Nup1 C-terminal residues 777-1076

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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LDB203

Plasmid

#185424

Purpose

Bacterial expression of N-terminus of NUP1 nucleoporin as GST-Nup1-N fusion

Depositor

Ken Belanger, Laura Davis

Article

Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

Insert

NUP1

Tags

GST

Expression

Bacterial

Mutation

Nup1 N-terminal domain only

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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LDB205

Plasmid

#185425

Purpose

Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion

Depositor

Ken Belanger, Laura Davis

Article

Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

Insert

NUP1

Tags

GST

Expression

Bacterial

Mutation

Nup1 C-terminal domain only

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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KBB101

Plasmid

#185064

Purpose

Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion truncated after the last FXFG repeat

Depositor

Ken Belanger

Article

Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

Insert

NUP1

Tags

GST

Expression

Bacterial

Mutation

Nup1 C-terminal region, truncated

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-mouse Mdm2 mutant M3 (399-489)-IRES-GFP

Plasmid

#180490

Purpose

Role of Mdm2 variants in tumorigenesis

Depositor

Martine Roussel

Article

Dang et al Cancer Res. 2002 Feb 15;62(4):1222-30.

Inserts

Mouse Mdm2 mutant
Mouse Mdm2 mutant

Expression

Mammalian

Mutation

399-489

Promoter

MSCV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-mouse Mdm2 mutant M2 (299-489)-IRES-GFP

Plasmid

#180489

Purpose

Role of Mdm2 variants in tumorigenesis

Depositor

Martine Roussel

Article

Dang et al Cancer Res. 2002 Feb 15;62(4):1222-30.

Insert

Mouse Mdm2 mutant

Use

Retroviral

Expression

Mammalian

Mutation

299-489

Promoter

MSCV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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CAG-loxP-3xpA-loxP-td-sfGFP

Plasmid

#180150

Purpose

td-sfGFP cre reporter

Depositor

Mario Capecchi

Article

Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

Insert

td-sfGFP

Use

Cre/Lox

Expression

Mammalian

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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CAG-loxP-3xpA-H2BsfGFP-tdTomato - linker E

Plasmid

#125797

Purpose

Histone H2B-superfolder GFP and tdTomato fused by unskippable linker

Depositor

Mario Capecchi

Article

Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

Insert

H2BsfGFP-tdTomato

Use

Cre/Lox

Expression

Mammalian

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pZS039

Plasmid

#134233

Purpose

For in vitro transcription of Miniata CENP-A

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

Miniata CENP-A n-terminus (nt 1-204, aa 1-68)

Tags

GST

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pZS077

Plasmid

#134235

Purpose

Bacterial expression of CENP-C

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

GST-CENP-C

Tags

GST

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCCG 306

Plasmid

#131167

Purpose

PCR template for genomic C-terminal Cub-R-URA3 tag, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutant

Depositor

Nils Johnsson

Article

Dunkler et al Methods Mol Biol. 2015;1346:151-68. doi:

Type

Empty backbone

Tags

CCG

Expression

Yeast

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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Yes-10kb

Plasmid

#112996

Purpose

P element vector for transposon insertion of the 10Kb Drosophila Amplicon in Follicle Cells (DAFC-62D) into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Xie et al Proc Natl Acad Sci U S A. 2008 Jul 15;10

Insert

10Kb DAFC-62D amplicon

Use

P insertion vector

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMD-SsoPCNA1

Plasmid

#107076

Purpose

S. solfataricus PCNA1

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA1

Use

Cloning vector

Promoter

SP6, lac

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMD-SsoPCNA3

Plasmid

#107078

Purpose

S. solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA3

Use

Cloning vector

Promoter

SP6, lac

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMD-MsePCNA3

Plasmid

#107075

Purpose

M. sedula PCNA3

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA3

Use

Cloning vector

Promoter

SP6, lac

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMD-SsoPCNA2

Plasmid

#107077

Purpose

S. solfataricus PCNA2

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA2

Use

Cloning vector

Promoter

SP6, lac

Available Since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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