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pLenti-CMV-FlaviA-mNeptune-puro

Plasmid

#140091

Purpose

Fluorescence activatable reporter of flavivirus NS2B-NS3 protease activity

Depositor

Jorge Luis Arias-Arias

Article

Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

Insert

FlaviA-mNeptune

Use

Lentiviral; High expression

Tags

mNeptune

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-ArgiNLS-AausFP1

Plasmid

#220595

Purpose

Constitutive expression of a single-cell discriminating version of AausFP1 fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

AausFP1

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

EF1a

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-pAce-Kv2.1PR

Plasmid

#195535

Purpose

Flp recombinase-dependent green fluorescent, positive response-polarity voltage indicator; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAce-Kv2.1 proximal restriction sequence

Use

AAV

Expression

Mammalian

Mutation

Ace-mNeon 78K, 81D, 92N, 178F; SY linker

Promoter

CAG

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.NES-jRCaMP1b.WPRE.SV40

Plasmid

#100851

Purpose

AAV expression of jRCaMP1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1 and AAV9

Insert

jRCaMP1b

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA AXL

Plasmid

#105932

Purpose

Mammalian expression of myc-His tagged AXL

Depositor

Rosa Marina Melillo

Article

Krishnamoorthy et al J Biol Chem. 2013 Jun 14;288(24):17481-9

Insert

AXL (AXL Human)

Tags

His and myc

Expression

Mammalian

Promoter

tet-inducible CMV

Available Since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-Flpo

Plasmid

#174378

Purpose

also known as: pAAV-synP-FLPo. AAV virus for expressing FLPo recombinase

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Has Service

AAV Retrograde

Insert

FLPo recombinase

Use

AAV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-GalT-IRES-Puromycin

Plasmid

#134862

Purpose

Expresses EGFP-tagged Golgi marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

beta 1,4-galactosyltransferase (B4GALT1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 83-398

Promoter

EF1a

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pGG-B1

Plasmid

#78504

Purpose

Trans plasmid for production of recombinant AAV-B1 vectors. Plasmid backbone may yield higher titers than pAR-B1 and encodes AAV2 Rep and AAV-B1 capsid

Depositor

Miguel Sena-Esteves

Article

Choudhury et al Mol Ther. 2016 Apr 27. doi: 10.1038/mt.2

Inserts

AAV2 Rep
AAV-B1 capsid

Use

AAV

Expression

Mammalian

Available Since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2t-Tubulin-C-18

Plasmid

#231779

Purpose

Visualization of tubulin in mammalian cells using mGold2t

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t-Tubulin-C-18 (TUBA1B Human)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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phage UbiC G3BP1-SNAP

Plasmid

#119949

Purpose

Lentiviral vector expressing G3BP1-SNAP fusion protein

Depositor

Jeffrey Chao

Article

Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

G3BP1 (G3BP1 Human)

Use

Lentiviral

Tags

SNAP

Expression

Bacterial and Mammalian

Promoter

UbiC

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNAFLAG-ATM-kd

Plasmid

#43908

Depositor

Stephen Elledge

Article

Cortez et al Science. 1999 Nov 5. 286(5442):1162-6.

Insert

ataxia telangiectasia mutated (ATM Human)

Tags

FLAG and His

Expression

Mammalian

Mutation

kinase dead (D2870A and N2875K)

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA3-AUP1-FLAG

Plasmid

#185336

Purpose

Mammalian expression of AUP1

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Tags

FLAG, 6xHis

Expression

Mammalian

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe-HA-Chk2-D311V

Plasmid

#41921

Depositor

Stephen Elledge

Article

Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

Insert

Chk2 (CHEK2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

D311V

Available Since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-hSynapsin1-FLEx-axon-GCaMP6s-P2A-mRuby3

Plasmid

#112008

Purpose

For cre-dependent enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Insert

axon-GCaMP6s-P2A-mRuby3

Use

AAV and Cre/Lox

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Promoter

hSynapsin1

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-ExRai-AKAR2(T/A)

Plasmid

#161754

Purpose

Negative-control mutant for ExRai-AKAR2 biosensor.

Depositor

Jin Zhang

Article

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Insert

ExRai-AKAR2(T/A)

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Clathrin-mGreenLantern

Plasmid

#164462

Purpose

Localization of mGreenLantern fluorescent protein to clathrin

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Tags

Clathrin

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

CMV

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pTP261

Plasmid

#104166

Purpose

Bacterial expression plasmid of anti-GFP nanobody with 1x Cysteine for maleimide labeling

Depositor

Dirk Görlich

Article

Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

Insert

Anti-GFP nanobody Enhancer PDB 3K1K (1x Cysteine)

Tags

14x Histidine tag and NEDD8 from Brachypodium dis…

Expression

Bacterial

Mutation

1x Cysteine located at bps 493-495 in full sequen…

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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peGFP C3-SPG20-PAAA

Plasmid

#218581

Purpose

Expresses GFP-tagged PPAY motif mutant (PAAA) of human SPG20 in mammalian cells; this mutant does not interact with ITCH

Depositor

Hemmo Meyer

Article

Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

Insert

SPG20 (SPART Human)

Tags

eGFP

Expression

Mammalian

Mutation

changed Proline 172 to Alanine and Tyrosine 174 t…

Promoter

CMV

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAcBac1.tR4-AcdRS82

Plasmid

#174101

Purpose

Expression of orthogonal Mb tRNA and Acd82 tRNA synthetase for incorporation of acridone in eukaryotic cells.

Depositor

Ryan Mehl

Article

Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

Insert

M. barkeri tRNA synthetase for acridone82

Expression

Mammalian

Mutation

N311S, C313G, V366A, W382T

Promoter

CMV

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40

Plasmid

#100849

Purpose

AAV expression of Cre recombinase-activated jRCaMP1b, a red fluorescent calcium sensor protein, from the CAG promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1

Insert

jRCaMP1b

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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