We narrowed to 10,664 results for: t7

1. pET-Himar-dCas9-mammalian

   Plasmid

   #138414

   Purpose

   Protein purification of Himar-dCas9 with 3x-FLAG, SV40 NLS, and 6x-His tag

   Depositor

   Harris Wang

   Article

   Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

   Insert

   Himar-dCas9
   

   Tags

   3xFLAG, 6xHis, and SV40 NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-AlpJ

   Plasmid

   #139598

   Purpose

   expresses AlpJ in E. coli

   Depositor

   Emily Balskus

   Article

   Wang et al J Am Chem Soc. 2017 Mar 1;139(8):2864-28

   Insert

   alpJ
   

   Tags

   N terminal hexahistidine

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-KinO1

   Plasmid

   #139597

   Purpose

   expresses KinO1 in E. coli

   Depositor

   Emily Balskus

   Article

   Wang et al J Am Chem Soc. 2017 Mar 1;139(8):2864-28

   Insert

   kinO1
   

   Tags

   N terminal hexahistidine

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-KinL

   Plasmid

   #139595

   Purpose

   expresses KinL in E. coli

   Depositor

   Emily Balskus

   Article

   Wang et al Nat Commun. 2018 Sep 11;9(1):3687. doi:

   Insert

   kinL
   

   Tags

   hexahistidine

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-KinM

   Plasmid

   #139594

   Purpose

   expresses KinM in E. coli

   Depositor

   Emily Balskus

   Article

   Wang et al Nat Commun. 2018 Sep 11;9(1):3687. doi:

   Insert

   kinM
   

   Tags

   hexahistidine

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-KinN

   Plasmid

   #139593

   Purpose

   expresses KinN in E. coli

   Depositor

   Emily Balskus

   Article

   Wang et al Nat Commun. 2018 Sep 11;9(1):3687. doi:

   Insert

   kinN
   

   Tags

   hexahistidine

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-SznF H407A H409A H448A

   Plasmid

   #139566

   Purpose

   expressed SznF H407A H409A H448A in E. coli

   Depositor

   Emily Balskus

   Article

   Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

   Insert

   SznF H407A H409A H448A
   

   Tags

   hexahistidine

   Expression

   Bacterial

   Mutation

   changed glutamate 215 to alanine

   Promoter

   T7

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pst39-NST-clone14

   Plasmid

   #133421

   Purpose

   S. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).

   Depositor

   Gerhard Wagner

   Insert

   NIP1/SUI1/TIF5 (NIP1, TIF5, SUI1 Budding Yeast)
   

   Expression

   Bacterial

   Mutation

   ORF for Nip1 encodes aa 4-156 (not complete ORF),…

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSB1C3-pT7-sfGFP_RED20

   Plasmid

   #135173

   Purpose

   Expresses sfGFP recoded in the RED20 genetic code off the T7 promoter

   Depositor

   Drew Endy

   Article

   Calles et al Nucleic Acids Res. 2019 Sep 12. pii: 556

   Insert

   sfGFP_RED20
   

   Use

   Synthetic Biology

   Mutation

   Recoded ORF of sfGFP to only use one codon per am…

   Promoter

   taatacgactcactata

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHBDuet259

    Plasmid

    #121911

    Purpose

    NpuDnaBΔ283ΔC39 intein

    Depositor

    Hideo Iwai

    Article

    Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

    Insert

    NpuDnaBΔ283ΔC39 intein
    

    Tags

    His6-GB1

    Expression

    Bacterial

    Mutation

    Deletion of 283-residue endonuclease domain

    Promoter

    T7

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZF-BBB

    Plasmid

    #127470

    Purpose

    coding sequence for synthetic zinc finger ZF-BBB

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-BBB synthetic transcription factor
    

    Use

    Cell-free transcription–translation

    Promoter

    T7

    Available Since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZF-BCC

    Plasmid

    #127484

    Purpose

    coding sequence for synthetic zinc finger ZF-BCC

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-BCC synthetic transcription factor
    

    Use

    Cell-free transcription–translation

    Promoter

    T7

    Available Since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBW016

    Plasmid

    #131820

    Purpose

    pET28b derrivative vector with T7-driven IPTG inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 55 and 56

    Depositor

    Jonathan Silberg

    Article

    Wu et al bioRxiv May 23, 2019

    Insert

    M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 55 and 56
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAc-Cdc3-Cdc10-SNAP

    Plasmid

    #131161

    Purpose

    Expression of Septin rods

    Depositor

    Nils Johnsson

    Article

    Renz et al BMC Biotechnol. 2013 Jul 26;13:60. doi:

    Insert

    Cdc3 + Cdc10-SNAP
    

    Use

    Bicistronic

    Tags

    Cdc10: SNAP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZF-ACA

    Plasmid

    #127466

    Purpose

    coding sequence for synthetic zinc finger ZF-ACA

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-ACA synthetic transcription factor
    

    Use

    Cell-free transcription–translation

    Promoter

    T7

    Available Since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZF-BCD

    Plasmid

    #127472

    Purpose

    coding sequence for synthetic zinc finger ZF-BCD

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-BCD synthetic transcription factor
    

    Use

    Cell-free transcription–translation

    Promoter

    T7

    Available Since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZF-BAB

    Plasmid

    #127469

    Purpose

    coding sequence for synthetic zinc finger ZF-BAB

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-BAB synthetic transcription factor
    

    Use

    Cell-free transcription–translation

    Promoter

    T7

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZF-BDC

    Plasmid

    #127473

    Purpose

    coding sequence for synthetic zinc finger ZF-BDC

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-BDC synthetic transcription factor
    

    Use

    Cell-free transcription–translation

    Promoter

    T7

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZF-BDD

    Plasmid

    #127474

    Purpose

    coding sequence for synthetic zinc finger ZF-BDD

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-BDD synthetic transcription factor
    

    Use

    Cell-free transcription–translation

    Promoter

    T7

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZF-ADD

    Plasmid

    #127468

    Purpose

    coding sequence for synthetic zinc finger ZF-ADD

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-ADD synthetic transcription factor
    

    Use

    Cell-free transcription–translation

    Promoter

    T7

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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