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pEF5/FRT-DEST EVC2ala5-TEV-YFP-Flag

Plasmid

#41012

Depositor

Rajat Rohatgi

Article

Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

Insert

Ellis van Creveld syndrome 2 (EVC2 Mouse)

Tags

TEV-YFP-Flag

Expression

Mammalian

Mutation

changed amino acids 1185-1188 to Alanine

Available Since

Nov. 15, 2012

Availability

Academic Institutions and Nonprofits only
pEF5/FRT-DEST EVC2ala4-TEV-YFP-Flag

Plasmid

#41011

Depositor

Rajat Rohatgi

Article

Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

Insert

Ellis van Creveld syndrome 2 (EVC2 Mouse)

Tags

TEV-YFP-Flag

Expression

Mammalian

Mutation

changed amino acids 1183-1186 to Alanine

Available Since

Nov. 15, 2012

Availability

Academic Institutions and Nonprofits only
pDest17_E3 ubiquitin-protein ligase (Topors) LCD (379-1045)

Plasmid

#235005

Purpose

Bacterial expression of N-terminally 6His tagged E3 ubiquitin-protein ligase (Topors) LCD

Depositor

Tanja Mittag

Insert

E3 ubiquitin-protein ligase (Topors) (TOPORS Human)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-DEST-Hsp70-zCreI-mTagBFP2d-2xins (JDW 1002)

Plasmid

#229817

Purpose

A gateway compatible Tol2 destination vector containing the Hsp70 promoter driving Cre and TagBFP followed by 2 cHS4 insulator cores.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

Tol2 destination vector

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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Tgfb-Induced-Enhancer:Beta-globin-minimal-promoter-EGFP:pA pDestTol2pA2

Plasmid

#220508

Purpose

TGFb-inducible fluorescent reporter (TIE:EGFP)

Depositor

Leonard Zon

Inserts

TGFb inducible enhancer
beta-globin minimal promoter
SV40 PolyA signal

Use

Zebrafish expression (tol2)

Tags

EGFP

Available Since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dEsCas13d-EGFP-NLS-3xFlag

Plasmid

#132410

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dEsCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

R295A; H300A; R849A; H854A

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+i)

Plasmid

#192123

Purpose

Lentiviral deliviery of OFF VIPER CAR (anti-CD19)-candidate design(d+i)

Depositor

Wilson Wong

Article

Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

Insert

pEF1a-anti-CD19scFV_CD28__41BB_peptide_P2A_DAP10_41BB_NS3(s139a)_CD3z-mCherry

Use

Lentiviral

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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HSP70l-APEX2-GBP in pDEST-Tol2-pA2-acrys-mCherry

Plasmid

#71282

Purpose

Zebrafish expression vector for heat-shock inducible expression of APEX2-GBP

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Use

Zebrafish expression with tol2

Promoter

HSP70l

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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Bact2-APEX2-GBP in pDEST-Tol2-pA2-acrys-cherry

Plasmid

#67668

Purpose

Zebrafish expression vector for ubiquitous expression of APEX2-GBP

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Use

Zebrafish expression with tol2

Available Since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

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Desmoplakin I acceptor only control (F40-based tension sensor)

Plasmid

#119189

Purpose

The acceptor (mEYFP) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration or with the donor only control to determine intermolecular FRET.

Depositor

Alex Dunn

Article

Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

Insert

human Desmoplakin I-[mTFP1(Y72G)-F40-mEYFP] (internal-1945) (DSP Human)

Use

Transposon

Tags

mTFP1-F40-mEYFP

Expression

Mammalian

Mutation

inserted F40-based tension sensor module after aa…

Promoter

TCE

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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