We narrowed to 5,377 results for: pcdna3.1
-
Plasmid#124494PurposeExpresses FlipGFP (caspase-1 cleavage sequence) and T2A mCherryDepositorInsertFlipGFP(Caspase-1 cleavage seq) T2A mCherry
UseSynthetic BiologyAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
NanoLuc-PM
Plasmid#164784PurposeNanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptorsDepositorInsertNanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Oplophorus gracilirostris, Human)
TagsNanoLucExpressionMammalianPromoterCMVAvailable SinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
OTR untagged
Plasmid#208913Purposeencodes the human oxytocin receptor, without any tagsDepositorAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
hHSF1 RNAi resistant
Plasmid#118347PurposeThis plasmid expresses RNAi resistance version of hHSF1 to use for rescue experiments with HSF1 shRNA vector (plasmid #118345).DepositorInserthHSF1 (HSF1 Human)
Tagsmyc and 6xHISExpressionMammalianMutationRNAi resistance mutations (does not change amino …Available SinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-his-BACH1 K52R
Plasmid#17643DepositorInsertBACH1 (BRIP1 Human)
TagsHis and MycExpressionMammalianMutationThe lysine residue at position 52 was changed to …Available SinceApril 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
CMV-CASANOVA
Plasmid#113035PurposeCASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9DepositorInsertCASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
UseCRISPR and Synthetic BiologyExpressionMammalianAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTFG011_Spike_Parental_D614G_3xFlag
Plasmid#191571PurposeSARS-CoV-2 Spike protein under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. For expression and pseudotyping lentiviral particles.DepositorInsertSARS-CoV-2 Spike Protein (S Synthetic)
Tags3x FLAGExpressionMammalianMutationD614G mutation. Last 19 amino acids truncated.PromoterCMVAvailable SinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mito-mGreenLantern
Plasmid#164461PurposeLocalization of mGreenLantern fluorescent protein to mitochondriaDepositorInsertmGreenLantern
TagsHuman COX8a first 29 amino acidsExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-mCherry-TAG-EGFP
Plasmid#232156PurposeA fluorescence reporter to show incorporation of target unnatural amino acids.DepositorInsertmCherry-linker(TAG)-EGFP
UseSynthetic BiologyExpressionMammalianAvailable SinceJuly 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 TAK1/F
Plasmid#44161DepositorAvailable SinceOct. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
FAT FAK biosensor
Plasmid#78303PurposeFRET biosensor. Visualization of FAK activity at focal adhesions in live cellsDepositorInsertFAT FAK biosensor
ExpressionMammalianPromoterCMVAvailable SinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/BLM
Plasmid#111766PurposeTo express human BLM protein in human cellsDepositorInsertBLM (BLM Human)
TagsFlag epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys…ExpressionMammalianPromoterCMVAvailable SinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
GLuc-PM
Plasmid#164783PurposeGaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptorsDepositorInsertGaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Human, Gaussia princeps)
TagsGLucExpressionMammalianPromoterCMVAvailable SinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pY094
Plasmid#84743PurposeExpresses huAsCpf1-T2A-GFP and crRNA guideDepositorInsertshuAsCpf1
GFP
Tags3xHA, NLS, and T2AExpressionMammalianPromoterCMVAvailable SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-SREBP-1 (A317-318)
Plasmid#204193PurposeMammalian expression of SREBP-1 (isoform 1)DepositorAvailable SinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-SREBP-1 (A317-318; A341)
Plasmid#204194PurposeMammalian expression of SREBP-1 (Isoform 1)DepositorInsertSREBP-1 (SREBF1 Human)
TagsE-tag and T7x2ExpressionMammalianMutationGly to Ala 317,318,341Available SinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
RF-118-UB-BRCA1
Plasmid#78960PurposeExpression of the C-terminal fragment of BRCA1 that is endogenously generated by caspase cleavage in mammalian cellsDepositorInsertBRCA1 (BRCA1 Human)
Tags3x Flag and ubiquitinExpressionMammalianMutationdelta 1-1155PromoterCMVAvailable SinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
CaMK2rep-S603A
Plasmid#239623PurposeInactive CaMKII activity reporter. The plasmid contains sGFP2 fluorescent protein, 3xmyc tags and double mutated phospho-site 3 from rat Synapsin-1a.DepositorInsertSpotNES-sGFP2-SynP3-3xmyc (Syn1 Rat, Synthetic)
TagsSpot, myc, and sGFP2ExpressionMammalianMutationchanged Serine 603 to AlaninePromoterCMVAvailable SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
WN10150
Plasmid#80443PurposeExpresses dead (908A) AsCpf1DepositorInsertnuclease dead (908A) AsCpf1
TagsNLS-3xHAExpressionMammalianMutationnuclease dead (D908A)Available SinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR149
Plasmid#106049PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR149; Opto-GPR149; E3)DepositorInsertOpto-GPR149 (GPR149 Bovine, Human)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only