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pAAV-U6sgp53-U6sgbbSapI-GFAPCre

Plasmid

#100275

Purpose

AAV-CRISPR mediated genome editing in glia / astrocytes

Depositor

Sidi Chen

Article

Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

Type

Empty backbone

Use

AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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NSK021 CMV-TO-p53R248Q (FLP-IN)

Plasmid

#191167

Purpose

Expression of human p53 mutant R248Q in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

p53(R248Q) (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

R248Q

Promoter

CMV-TO

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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IRSp53/BAIAP2 scFv [L117/1]

Plasmid

#190506

Purpose

Mammalian Expression of IRSp53/BAIAP2 scFV. Derived from hybridoma L117/1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

IRSp53/BAIAP2 (Homo sapiens) recombinant scFV (BAIAP2 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-TP53RK_IDG-K

Plasmid

#170589

Purpose

Gateway destination clone of TP53RK (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-TP53RK (TP53RK Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pMKO.1 puro p53 shRNA 2

Plasmid

#10672

Depositor

William Hahn

Article

Masutomi et al Cell. 2003 Jul 25. 114(2):241-53.

Insert

p53 shRNA (TP53 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

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pRRLNeo-pEF1a-p53ashL344A-mCerulean

Plasmid

#69580

Purpose

Expresses p53-L344A mutant tagged with mCerulean

Depositor

Galit Lahav

Article

Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

Insert

p53-L344A (TP53 Human)

Use

Lentiviral

Tags

mCerulean

Expression

Mammalian

Mutation

p53 L344A mutation that prevents tetramerization …

Promoter

EF1alpha

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pIRES2-mCherry-p53 deltaN

Plasmid

#49243

Purpose

expresses human p53 deltaN and mCherry in mammalian cells

Depositor

Dylan Taatjes

Article

Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

Insert

p53 deltaN (TP53 Human)

Use

Tags

IRES-mCherry

Expression

Mammalian

Mutation

deltaN ( lacks 39 residues at the N-terminus)

Promoter

CMV

Available since

Dec. 27, 2013

Availability

Academic Institutions and Nonprofits only

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HA-HIF2α P531A-pBabe-puro

Plasmid

#26091

Depositor

William Kaelin

Article

Kondo et al Cancer Cell. 2002 Apr . 1(3):237-46.

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA with point mutation P531A

Promoter

Available since

Sept. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pET28a SUMO Nup53xl 1-320

Plasmid

#85998

Purpose

bacterial expression of his6 and SUMO tagged Xenopus laevis Nup53

Depositor

Wolfram Antonin

Article

Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

Insert

Xenopus laevis Nup53 (nup35.L Frog)

Use

Tags

SUMO

Expression

Bacterial

Mutation

codon optimzed for bacterial expression

Promoter

T7

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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Degron-KI p53 donor vector

Plasmid

#65619

Purpose

Donor vector to generate Degron-KI DD-p53

Depositor

Frank Stegmeier

Article

Zhou et al Cancer Res. 2015 Mar 18. pii: canres.293

Insert

p53 Degron-KI left and right HR arms

Use

Tags

Expression

Mutation

Promoter

Available since

July 13, 2015

Availability

Academic Institutions and Nonprofits only