We narrowed to 1,212 results for: Ncl

Showing: 661 - 690 of 1212 results

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pJH126

Plasmid

#172147

Purpose

Microscopy inclusion body assay

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

pCDF.SUMO-B.(SD4).CD

Expression

Bacterial

Mutation

PylBCD operon (SUMO tag inserted for PylB; RBS in…

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pEE22

Plasmid

#161787

Purpose

Hi-SC2 reporter delivery plasmid

Depositor

François-Xavier Barre

Article

Espinosa et al Mol Cell. 2020 Sep 3;79(5):857-869.e3. d

Insert

Hi-SC2 reporter

Use

Conjugative plasmid to deliver hi-sc2 reporter

Mutation

Himar site included MmeI site, tandem loxP sites,…

Promoter

n/a

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pXF90

Plasmid

#139044

Purpose

MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

halotag::HDEL (includes STOP codon, PATC introns)

Expression

Worm

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLY131

Plasmid

#130956

Purpose

Engineered sgRNA-LEB2 generator including Anderson promoter J23100 for golden gate assembly

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB2

Use

Synthetic Biology

Expression

Bacterial

Promoter

Anderson promoter J23100

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLY76

Plasmid

#130963

Purpose

Engineered sgRNA-LEA2 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEA2

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plux2

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLY74

Plasmid

#130945

Purpose

Engineered sgRNA-G6 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-G6

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plux2

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLY111

Plasmid

#130942

Purpose

Engineered sgRNA-LEB4 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB4

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plux2

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLY110

Plasmid

#130940

Purpose

Engineered sgRNA-LEB3 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB3

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plux2

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLY77

Plasmid

#130964

Purpose

Engineered sgRNA-LEB2 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB2

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plux2

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLY124

Plasmid

#130946

Purpose

Engineered sgRNA-G6-2MS2 (2 MS2 aptamers) generator circuit including promoter Pbad

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-G6-2MS2

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pbad

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLY112

Plasmid

#130944

Purpose

Engineered sgRNA-LEB5 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB5

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plux2

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLY107

Plasmid

#130943

Purpose

Engineered sgRNA-LEA5 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEA5

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plux2

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLY105

Plasmid

#130939

Purpose

Engineered sgRNA-LEA3 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEA3

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plux2

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLY106

Plasmid

#130941

Purpose

Engineered sgRNA-LEA4 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEA4

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plux2

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(2)_T2AGeneSwitch

Plasmid

#125216

Purpose

Artificial phase 2 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GeneSwitch

Use

Other

Tags

T2A

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(2)_T2AGAL80

Plasmid

#125219

Purpose

Artificial phase 2 exon to include a spliced T2A-GAL80 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GAL80

Use

Other

Tags

T2A

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(1)_T2AGeneSwitch

Plasmid

#125215

Purpose

Artificial phase 1 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GeneSwitch

Use

Other

Tags

T2A

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMDR1-4

Plasmid

#69485

Purpose

Deletion Construct

Depositor

Kathleen Scotto

Article

Jin et al Mol Cell Biol. 1998 Jul;18(7):4377-84.

Insert

MDR1 promoter (ABCB1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Insert includes MDR1 promoter sequence from -4 to…

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only
GLRX3

Plasmid

#39036

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

GLRX3 (GLRX3 Human)

Tags

His Tev

Expression

Bacterial

Mutation

A PCR artifact adds a C-terminal sequence includi…

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pLG-deltaA

Plasmid

#33151

Depositor

Michael Rosbash

Article

Legrain et al Cell. 1989 May 19;57(4):573-83.

Insert

RP51A-synthetic minimal intron (RPS17A Budding Yeast)

Tags

LacZ

Expression

Yeast

Mutation

The RP51A sequence was modified by digestion with…

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pFloxin-Ofd1-Myc-S437R

Plasmid

#24577

Depositor

Jeremy Reiter

Article

Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Insert

Ofd1 (Ofd1 Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

Myc

Mutation

Serine 437 changed to Arginine (codon AGT to CGT)…

Available Since

May 27, 2010

Availability

Academic Institutions and Nonprofits only

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p077 pCS xTRPS1 (M)

Plasmid

#11047

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

TRPS1 mut (trps1 Frog)

Use

Mammalian, avian, and zebrafish

Mutation

Two Cys residues in the GATA-type zinc finger wer…

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
pCI SMN2

Plasmid

#72287

Purpose

SMN2 exon 7 splicing cassette

Depositor

Elliot Androphy

Article

Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

Insert

SMN2 (SMN2 Human)

Expression

Mammalian

Mutation

contains exons 6-7-8 including introns, multiple …

Promoter

CMV

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti TurboRFP BlastR

Plasmid

#102343

Purpose

Lentiviral expression of cytoplasmic TurboRFP with Blasticidin selection in cells including neurons

Depositor

Ghassan Mouneimne

Article

Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

Insert

TurboRFP

Use

Lentiviral

Tags

N/A

Promoter

CMV immediate early

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-HaloTag-CNA35

Plasmid

#215457

Purpose

Encodes the bacterial collagen-binding protein CNA35 with N-terminal HaloTag.

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

CNA35

Tags

HaloTag

Expression

Bacterial

Mutation

Includes stop codon

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLX-TRV2

Plasmid

#180516

Purpose

Agrobacterium tumefaciens-based expression of TRV2 including a PEBV heterologous promoter and a polylinker

Depositor

Jose-Antonio Daros

Article

Aragones et al Biotechnol J. 2022 Mar 24:e2100504. doi:

Insert

Tobacco rattle virus RNA2 with a PEBV heterologous promoter and a polylinker

Expression

Plant

Promoter

35S CaMV

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pRDV5 containing Sb-loop

Plasmid

#132695

Purpose

pRDV5 plasmid containing a loop Sybody for the generation of a qPCR standard curve

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

Sb_loop including 5' and 3' flanking sequences used for ribosome display

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pRDV5 containing Sb-convex

Plasmid

#132696

Purpose

pRDV5 plasmid containing a convex Sybody for the generation of a qPCR standard curve

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

Sb_convex including 5' and 3' flanking sequences used for ribosome display

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQAT51

Plasmid

#223423

Purpose

T-DNA vector for LbCas12a-RRV based mutagenesis for dicot plants; highly efficient including TTV PAM; LbCas12a-RRV and the crRNA were driven by separate 2x35s promoter; Kanamycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

2x35s-LbCas12a-RRV-2x35s-crRNA scaffold

Use

CRISPR

Tags

NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pTara:500

Plasmid

#60717

Purpose

Expresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORF

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

T7 RNAP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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