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We narrowed to 4,045 results for: golden gate

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Showing: 1101 - 1150 of 4045 results

  1. pAV10.HO3.loxP

    Plasmid
    #63205
    Purpose
    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63205-plasmid-map-sequence-id-104905

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  2. pAV10.HO6

    Plasmid
    #63211
    Purpose
    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into HO locus (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63211-plasmid-map-sequence-id-115802

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  3. pAIP4_1UXZ

    Plasmid
    #234166
    Purpose
    Heterologous protein expression of cellulase B in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1UXZ
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234166-plasmid-map-sequence-id-464132

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  4. pKS1576

    Plasmid
    #239524
    Purpose
    Express Vba2p membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    ScVba2p-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239524-plasmid-map-sequence-id-475895

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  5. pHIE096 CMV Bxb1

    Plasmid
    #139252
    Purpose
    Encodes a Bxb1 recombinase for Landing Pad integration.
    Depositor
    Joshua Leonard
    Insert
    Bxb1 Recombinase
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    139252-plasmid-map-sequence-id-325167

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  6. pPD1019 TRE3GV DsRedExpress2 in TU2

    Plasmid
    #139254
    Purpose
    TRE3GV promoter and DsRedExpress2 in TUPV2
    Depositor
    Joshua Leonard
    Insert
    DsRedExpress2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3GV
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    139254-plasmid-map-sequence-id-325170

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  7. pJM467 TRE3GV EYFP in TU3

    Plasmid
    #139255
    Purpose
    TRE3GV promoter and EYFP in TUPV3
    Depositor
    Joshua Leonard
    Insert
    EYFP
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3GV
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    139255-plasmid-map-sequence-id-325172

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  8. pKS1338

    Plasmid
    #239511
    Purpose
    Express UTR1 membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    AtUTR1-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    Nov. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    239511-plasmid-map-sequence-id-489601

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  9. pKS1337

    Plasmid
    #239512
    Purpose
    Express Yea4p membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    ScYea4p-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    Nov. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    239512-plasmid-map-sequence-id-489589

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  10. pKS1335

    Plasmid
    #239509
    Purpose
    Express Ymd8p membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    ScYmd8p-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239509-plasmid-map-sequence-id-491723

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  11. pKS1336

    Plasmid
    #239510
    Purpose
    Express Hut1p membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    ScHut1p-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    239510-plasmid-map-sequence-id-487900

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  12. PACE_AP_Neg_pBADProm

    Plasmid
    #242141
    Purpose
    pBAD-SD8-GIII Neg; AP Neg Circuit for AraC phage-assisted evolution
    Depositor
    Dominik Niopek
    Insert
    Gene III Negative
    Expression
    Bacterial
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242141-plasmid-map-sequence-id-486018

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  13. PACE_AP_Pos_pBADProm

    Plasmid
    #242139
    Purpose
    pBAD-sd8-GIII Cassette; AP Pos Circuit for AraC phage-assisted evolution
    Depositor
    Dominik Niopek
    Insert
    Gene III
    Expression
    Bacterial
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242139-plasmid-map-sequence-id-486013

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  14. pKS1466

    Plasmid
    #239516
    Purpose
    Express Idi1p and Erg20WW for synthesis of GPP in the peroxisome; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    ScErg20p(F96W_N127W)-ePTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239516-plasmid-map-sequence-id-475876

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  15. pKS1137

    Plasmid
    #239498
    Purpose
    Express GAL-inducible peroxisomal sfCherry3C large fragment and inactive Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-ePTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239498-plasmid-map-sequence-id-475872

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  16. pKS1135

    Plasmid
    #239497
    Purpose
    Express synthetic sfCherry3C small fragment membrane protein cargo and peroxisomal mTagBFP2
    Depositor
    John Dueber
    Insert
    Ost1ss-sfCherry3C(11)-Pex3_TMD(+/-6AA)-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239497-plasmid-map-sequence-id-475868

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  17. pKS1169

    Plasmid
    #239503
    Purpose
    Express GAL-inducible cytosolic sfCherry3C large fragment and inactive Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-dPTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239503-plasmid-map-sequence-id-475865

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  18. pKS1025

    Plasmid
    #239496
    Purpose
    Express inactive peroxisomal RsAS as negative control
    Depositor
    John Dueber
    Insert
    Ubi-Y-RsAS(H360E)-Venus-ePTS1
    Expression
    Yeast
    Mutation
    H360E
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239496-plasmid-map-sequence-id-475861

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  19. pAIP4_3S60

    Plasmid
    #234231
    Purpose
    Heterologous protein expression of lectin OAA in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3S60
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 7, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234231-plasmid-map-sequence-id-464330

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  20. pAIP4_5M2Y

    Plasmid
    #234193
    Purpose
    Heterologous protein expression of B7LG64_ECO55 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5M2Y
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234193-plasmid-map-sequence-id-464158

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  21. pAIP4_3H6Q

    Plasmid
    #234185
    Purpose
    Heterologous protein expression of macrocypin-1a in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3H6Q
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234185-plasmid-map-sequence-id-464150

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  22. pAIP4_4Q2S

    Plasmid
    #234177
    Purpose
    Heterologous protein expression of C20G4.08 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4Q2S
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234177-plasmid-map-sequence-id-464142

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  23. pAIP4_4O6G

    Plasmid
    #234175
    Purpose
    Heterologous protein expression of uncharacterized protein in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4O6G
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234175-plasmid-map-sequence-id-464140

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  24. pAIP4_3K1H

    Plasmid
    #234157
    Purpose
    Heterologous protein expression of O25709_HELPY in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3K1H
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234157-plasmid-map-sequence-id-464123

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  25. PT7-alphaHL

    Plasmid
    #227650
    Purpose
    pT7-driven expression of alpha-hemolysin, a pore-forming protein in lipid bilayers.
    Depositor
    Richard Murray
    Insert
    alpha-hemolysin
    Use
    Synthetic Biology
    Available Since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    227650-plasmid-map-sequence-id-452398

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  26. pBD006

    Plasmid
    #201306
    Purpose
    D16: Promoter of TraesCS5D02G320700 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D16: Promoter of TraesCS5D02G320700
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    201306-plasmid-map-sequence-id-409554

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  27. pBD004

    Plasmid
    #201304
    Purpose
    D14: Promoter of TraesCS3D02G368800 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D14: Promoter of TraesCS3D02G368800
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    201304-plasmid-map-sequence-id-409541

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  28. pBD005

    Plasmid
    #201305
    Purpose
    D15: Promoter of TraesCS3D02G517500 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D15: Promoter of TraesCS3D02G517500
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201305-plasmid-map-sequence-id-409522

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  29. pPD1023 TRE3GV DsRedExpress2 in TU5

    Plasmid
    #139257
    Purpose
    TRE3GV promoter and DsRedExpress2 in TUPV5
    Depositor
    Joshua Leonard
    Insert
    DsRedExpress2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3GV
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    139257-plasmid-map-sequence-id-325370

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  30. C77m

    Plasmid
    #120989
    Purpose
    MoClo golden gate assembly CD partfor AmpR (ampicillin/carbenicillin resistance protein). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    AmpR
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120989-plasmid-map-sequence-id-236056

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  31. C78m

    Plasmid
    #120990
    Purpose
    MoClo golden gate assembly CD cloning part for ChlorR (chloramphenicol resistance protein). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ChlorR
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120990-plasmid-map-sequence-id-236059

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  32. pET26b-BsaI

    Plasmid
    #233523
    Purpose
    Purification vector with BsaI cut sites for streamlined cloning
    Depositor
    Carl Denard
    Type
    Empty backbone
    Tags
    6xHis-tag and pelB leader
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    233523-plasmid-map-sequence-id-474147

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  33. C80m

    Plasmid
    #120992
    Purpose
    MoClo golden gate assembly CD part for mfLon protease (see doi: [10.1038/nbt.3053]). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    mfLon protease
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120992-plasmid-map-sequence-id-236095

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  34. pICSL11085_GG_Wheat_live_Cas9_for

    Plasmid
    #126881
    Purpose
    Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    126881-plasmid-map-sequence-id-249630

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  35. C86m

    Plasmid
    #120997
    Purpose
    MoClo golden gate assembly CD part (phage lysis protein; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    X174E
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120997-plasmid-map-sequence-id-236126

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  36. C11m

    Plasmid
    #120959
    Purpose
    MoClo golden gate assembly CD part for Citrine (modified YFP; variant of https://www.fpbase.org/protein/citrine/) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    Citrine
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120959-plasmid-map-sequence-id-238041

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  37. C5m

    Plasmid
    #120957
    Purpose
    MoClo golden gate assembly CD part for mRFP (monomeric red fluorescent protein; see https://doi.org/10.1073/pnas.082243699 and https://www.fpbase.org/protein/mrfp1/).
    Depositor
    Richard Murray
    Insert
    mRFP
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120957-plasmid-map-sequence-id-236154

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  38. C115m

    Plasmid
    #121014
    Purpose
    MoClo golden gate assembly CD part for TP901 integrase (Integrase from lactococcal phage TP901). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    TP901 integrase
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121014-plasmid-map-sequence-id-236321

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  39. pL0M-SC-RUBY

    Plasmid
    #245485
    Purpose
    Golden Gate Level 0 "SC" RUBY module
    Depositor
    Beth Rowan
    Insert
    RUBY
    Expression
    Plant
    Available Since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    245485-plasmid-map-sequence-id-488120

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  40. pDest_PANCEd_AraC_sd2_R5_C1

    Plasmid
    #242150
    Purpose
    Expression Plasmid containing a LacI repressed sd2 AraC R5 PANCE variant cassette
    Depositor
    Dominik Niopek
    Insert
    AraC R5
    Expression
    Bacterial
    Mutation
    A2E, L156I, R251H
    Available Since
    Aug. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    242150-plasmid-map-sequence-id-484658

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  41. pKS1170

    Plasmid
    #239504
    Purpose
    Express GAL-inducible cytosolic sfCherry3C large fragment and Pex22 peroxisomal chauffeur with medium affinity SH3 peptide
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-dPTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239504-plasmid-map-sequence-id-475911

    Enlarge

  42. pKS1171

    Plasmid
    #239505
    Purpose
    Express GAL-inducible cytosolic sfCherry3C large fragment and Pex22 peroxisomal chauffeur with high affinity SH3 peptide
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-dPTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239505-plasmid-map-sequence-id-475889

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  43. pKS1517

    Plasmid
    #239518
    Purpose
    Express ClPT1(37-407) membrane enzyme cargo and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    2xCrk_SH3-mTurquoise2-ClPT1(37-407)
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239518-plasmid-map-sequence-id-475888

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  44. pKS1520

    Plasmid
    #239520
    Purpose
    Express CpPT1(52-408) membrane enzyme cargo and inactive Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    2xCrk_SH3-mTurquoise2-CpPT1(52-408)
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239520-plasmid-map-sequence-id-475881

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  45. pKS1518

    Plasmid
    #239519
    Purpose
    Express CpPT1(52-408) membrane enzyme cargo and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    2xCrk_SH3-mTurquoise2-CpPT1(52-408)
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239519-plasmid-map-sequence-id-475880

    Enlarge

  46. pKS1138

    Plasmid
    #239499
    Purpose
    Express GAL-inducible peroxisomal sfCherry3C large fragment and Pex22 peroxisomal chauffeur with medium affinity SH3 peptide
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-ePTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239499-plasmid-map-sequence-id-475874

    Enlarge

  47. pKS1139

    Plasmid
    #239500
    Purpose
    Express GAL-inducible peroxisomal sfCherry3C large fragment and Pex22 peroxisomal chauffeur with high affinity SH3 peptide
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-ePTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239500-plasmid-map-sequence-id-475873

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  48. pAIP4_2H7W

    Plasmid
    #234220
    Purpose
    Heterologous protein expression of chagasin in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2H7W (Tc00.1047053506801.80 Trypanosoma cruzi)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234220-plasmid-map-sequence-id-464185

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  49. pAIP4_2NT3

    Plasmid
    #234208
    Purpose
    Heterologous protein expression of response regulator homolog in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2NT3
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234208-plasmid-map-sequence-id-464173

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  50. U22m

    Plasmid
    #121025
    Purpose
    MoClo golden gate assembly BC part (high expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD6
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121025-plasmid-map-sequence-id-245900

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Showing: 1101 - 1150 of 4045 results