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We narrowed to 689 results for: intein

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Showing: 441 - 460 of 689 results

  5. IBMc226

    Plasmid
    #161944
    Purpose
    Contains Type IIS restriction site free elements for expressing protein to be split in IBM. TetR expression plasmid
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR-Ter
    Expression
    Bacterial
    Promoter
    PBAD
    Available Since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    161944-plasmid-map-sequence-id-326341

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  6. IBMc397

    Plasmid
    #161955
    Purpose
    Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to acVHH
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T
    Expression
    Bacterial
    Promoter
    PBAD
    Available Since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    161955-plasmid-map-sequence-id-326361

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  7. IBMc227

    Plasmid
    #161948
    Purpose
    Contains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on TetR
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR(1-20)-BsaI-BsaI-TetR(188-207)-Ter
    Expression
    Bacterial
    Promoter
    PBAD
    Available Since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    161948-plasmid-map-sequence-id-326357

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  8. IBMc367

    Plasmid
    #161947
    Purpose
    Contains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on ECF20
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1C3(BsmBI-,BsaI+)-BsaI-ECF20(6-188)-BsaI_v3
    Expression
    Bacterial
    Available Since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    161947-plasmid-map-sequence-id-326355

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  9. IBMc228

    Plasmid
    #161946
    Purpose
    Contains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on TetR
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1C3(BsmBI-,BsaI+)-BsaI-TetR(20-188)-BsaI
    Expression
    Bacterial
    Available Since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    161946-plasmid-map-sequence-id-326343

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  10. pANY8

    Plasmid
    #164900
    Purpose
    Containing His-ELP-intein multifunctional tag for protein purification by combining Ni-NTA-based and ELP-based strategy with a specially designed purification protocol.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Tags
    His-ELP-Intein-tag
    Expression
    Bacterial
    Available Since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    164900-plasmid-map-sequence-id-330483

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  12. pJK237

    Plasmid
    #73339
    Purpose
    Produces Acetobacter aceti 1023 citrate synthase with cleavable N-terminal His6/CBD/Ssp-intein fusion
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Tags
    His6, Ssp intein, and chitin-binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    73339-plasmid-map-sequence-id-138207

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  13. pET30a-HS-Tn5

    Plasmid
    #127916
    Purpose
    Prokaryotic expression of Hyperstable Tn5 (HS-Tn5) for Illumina library preperation. Developed for the large scale ChIP-Seq and ATAC-Seq experiments for the fruitENCODE and Maize Cistrome projects.
    Depositor
    Silin Zhong
    Article
    Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:
    Insert
    Hyperstable Tn5
    Tags
    E coli elongation factor Ts and Mxe intein - Chit…
    Expression
    Bacterial
    Mutation
    E54K, L372P
    Promoter
    T7
    Available Since
    July 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    127916-plasmid-map-sequence-id-248754

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  17. pcDNA3-FRB-VMAc-CSarcin

    Plasmid
    #70223
    Purpose
    Expresses VMA intein with C-terminal portion of alpha-sarcin
    Depositor
    Perry Howard
    Article
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Insert
    alpha Sarcin (C-terminal portion)
    Tags
    FKBP-rapamycin binding (FRB) domain of the mammal…
    Expression
    Mammalian
    Mutation
    Changed Asp 28 to Cys, deleted amino acids 1-27
    Promoter
    CMV
    Available Since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. pTXB1-CI

    Plasmid
    #51565
    Purpose
    Expression of bacteriophage lambda CI for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone
    Depositor
    Sankar Adhya, Francisco Malagon
    Insert
    cI
    Tags
    Intein-Chiting Binding Domanin
    Expression
    Bacterial
    Available Since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51565-plasmid-map-sequence-id-78820

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  20. pcDNA3-NGFP-VMAn-FKBP

    Plasmid
    #70225
    Purpose
    Expresses N-terminal portion of GFP along with VMA intein for protein splicing
    Depositor
    Perry Howard
    Article
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Insert
    eGFP
    Tags
    FK506- and rapamycin-binding protein (FKBP), Link…
    Expression
    Mammalian
    Mutation
    Changed Glu 125 to Ile, Deleted amino acids 129-2…
    Promoter
    CMV
    Available Since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 441 - 460 of 689 results