We narrowed to 1,516 results for: pet-28a

1. pET28a-DONSON-DEW

   Plasmid

   #208802

   Purpose

   Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A)

   Depositor

   Aga Gambus

   Article

   Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

   Insert

   DONSON (donson.L Frog)
   

   Use

   Tags

   His and Myc

   Expression

   Bacterial

   Mutation

   D374A; E377A; W381A

   Promoter

   T7

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-DONSON-S437A

   Plasmid

   #208803

   Purpose

   Xenopus laevis His6-Myc-DONSON (S437A)

   Depositor

   Aga Gambus

   Article

   Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

   Insert

   DONSON (donson.L Frog)
   

   Use

   Tags

   His and Myc

   Expression

   Bacterial

   Mutation

   S437A

   Promoter

   T7

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-DONSON-ΔD1

   Plasmid

   #208804

   Purpose

   Xenopus laevis His6-DONSON (1-155aa removed)

   Depositor

   Aga Gambus

   Article

   Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

   Insert

   DONSON (donson.L Frog)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   ΔD1, deletion of 1-155aa

   Promoter

   T7

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-6xHis-TrxF

   Plasmid

   #207454

   Purpose

   E. coli expression of Mesembryanthemum crystallinum Thioredoxin F with thrombin-cleavable N-terminal 6xHis tag

   Depositor

   Sarah Stainbrook, Joseph Jez

   Article

   Stainbrook et al bioRxiv 2022.08.24.505184

   Insert

   Thioredoxin F
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for E coli expression

   Promoter

   T7

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a LZ control-FLAG

   Plasmid

   #199678

   Purpose

   Bacterial expression of Leucine Zipper (LZ) control-Flag

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   Leucine zipper control
   

   Use

   Tags

   6xHis and Flag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a LZ LED WT-FLAG

   Plasmid

   #199679

   Purpose

   Bacterial expression of Leucine Zipper (LZ) LYCHOS LED-Flag

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   Leucine zipper LYCHOS LED WT (GPR155 Human)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a Cdk2ap1CAN-MutTER

   Plasmid

   #178033

   Purpose

   Expression vector for the purpose of protein purification.

   Depositor

   Lin He

   Article

   Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

   Insert

   Cdk2ap1 (Cdk2ap1 Mouse)
   

   Use

   Nonviral

   Tags

   6xHIS - Thrombin - MBP- TEV-TRS

   Expression

   Bacterial

   Mutation

   Changed T(108)A, E(109)A, R(110)A

   Promoter

   T7LacI

   Available Since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-matNODALvar-HIS

   Plasmid

   #115265

   Purpose

   For bacterial recombinant protein production of the mature peptide of a human NODAL splice variant

   Depositor

   Lynne Postovit

   Article

   Findlay et al (2018) bioRxiv 276170

   Insert

   NODAL variant mature peptide (NODAL Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-eGFP-Pep-H

   Plasmid

   #185788

   Purpose

   Encodes enhanced green flourescent protein linked to Pep-H self-assembling peptide via a GS-linker sequence

   Depositor

   Lizhong He

   Article

   Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

   Insert

   eGFP-Pep4
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a Cdk2ap1ΔN (MT2B2)

    Plasmid

    #178034

    Purpose

    Expression vector for the purpose of protein purification.

    Depositor

    Lin He

    Article

    Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

    Insert

    Cdk2ap1 (Cdk2ap1 Mouse)
    

    Use

    Nonviral

    Tags

    6xHIS - Thrombin - MBP- TEV-TRS

    Expression

    Bacterial

    Mutation

    First N-Terminal 27aa are deleted

    Promoter

    T7LacI

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. 6xHis-CPL2/pET28a

    Plasmid

    #172095

    Purpose

    cDNA of CER11/CPL2 cloned into pET28a for protein expression

    Depositor

    George Haughn

    Article

    Shi et al Plant Physiol. 2019 Nov;181(3):901-915.

    Insert

    CPL2 (CPL2 Mustard Weed)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pET28a-XPO5-His6

    Plasmid

    #171562

    Purpose

    Encodes human XPO5 protein

    Depositor

    Yan Zeng

    Insert

    XPO5 (XPO5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a SznF E281A

    Plasmid

    #139591

    Purpose

    expresses SznF E281A

    Depositor

    Emily Balskus

    Article

    Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

    Insert

    SznF E281A
    

    Use

    Tags

    hexahistidine

    Expression

    Bacterial

    Mutation

    changed glutamate 281 to alanine

    Promoter

    T7

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a SznF H225A

    Plasmid

    #139590

    Purpose

    expresses SznF H225A

    Depositor

    Emily Balskus

    Article

    Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

    Insert

    SznF H225A
    

    Use

    Tags

    hexahistidine

    Expression

    Bacterial

    Mutation

    changed histidine 225 to alanine

    Promoter

    T7

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-SznF E215A

    Plasmid

    #139565

    Purpose

    expresses SznF E215A in E. coli

    Depositor

    Emily Balskus

    Article

    Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

    Insert

    SznF E215A
    

    Use

    Tags

    hexahistidine

    Expression

    Bacterial

    Mutation

    changed glutamate 215 to alanine

    Promoter

    T7

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-DDX43-NT-G46D

    Plasmid

    #134588

    Purpose

    Express human DDX43 N-terminal region (1-253 aa, with G46D mutation) in E. coli

    Depositor

    Yuliang Wu

    Article

    Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

    Insert

    DDX43-N-terminal (1-253 aa) with G46D mutation (DDX43 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    full-length (G154D)

    Promoter

    T7

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-DDX43-FL-G84D

    Plasmid

    #134577

    Purpose

    Express human DDX43 mutant (full-length with G84D mutation) in E. coli

    Depositor

    Yuliang Wu

    Article

    Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

    Insert

    DDX43-G84D (DDX43 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    full-length (G84D)

    Promoter

    T7

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-clbH-C-A2-PCP

    Plasmid

    #114157

    Purpose

    Expresses ClbH-C-A2-PCP

    Depositor

    Emily Balskus

    Article

    Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

    Insert

    clbH
    

    Use

    Tags

    6xHis-thrombin-T7 tag

    Expression

    Bacterial

    Mutation

    Only contains the C-A2-PCP domains of ClbH

    Promoter

    Available Since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a_6HT-TBP6.7(P46A)

    Plasmid

    #112721

    Purpose

    This mutation (P46A) marks the first residue within the evolved region of U1A.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(P46A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    P46A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a_6HT-TBP6.7(R47A)

    Plasmid

    #112722

    Purpose

    Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R47A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R47A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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