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Plasmid#132511PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertZNF624 (ZNF624 Human)
UseCRISPRTagsGFPExpressionMutationnonePromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZNF8-donor
Plasmid#136689PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertZNF8 (ZNF8 Human)
UseCRISPRTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
LncRNA overexpression EF1α_BbsI_SV40 polyA_EGFP
Plasmid#219828PurposeOverexpression vector for non-coding RNAs driven by EF1α promoter with GFP. Cloning using BbsI.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 31, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
AbVec2.0-mIghg3
Plasmid#127160PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG3 isotypeDepositorInsertIghg3 (Ighg3 Mouse)
UseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPATZ1-donor
Plasmid#132522PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertPATZ1 (PATZ1 Human)
UseCRISPRTagsGFPExpressionMutationnonePromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(30-120)
Plasmid#191391PurposeDonor for targeted integration of hCdt1(30-120) probe to the hROSA26 safe harbor locusDepositorInserthCdt1(30-120)
UseCRISPR and Synthetic BiologyTagsmScarlet-IExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)
Plasmid#191389PurposeDonor for targeted integration of hCdt1(1-100) probe to the hROSA26 safe harbor locusDepositorInserthCdt1(1-100)
UseTagsmScarlet-IExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
hSLBP_mNG_FS_Donor
Plasmid#191387PurposeDonor for C-terminal tagging of endogenous hSLBPDepositorInsertmNeonGreen(mNG) + FS(3xFLAG_TwinStrep)Tag for hSLBP tagging at the C-terminus (SLBP Synthetic, Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pUC119
Plasmid#50010PurposepUC cloning vectorDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pINER5
Plasmid#185895PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).DepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PSk.GAL2>YFAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 25, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUA97
Plasmid#69340PurposeIt encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategyDepositorInsertceBFP SEC 3xFlag
UseTags3xFlag and ceBFP (mTagBFP)ExpressionWormMutationsqt-1(e1350)PromoterAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLinker-6xHA-HXGPRT-LoxP
Plasmid#86552PurposeThe plasmid is used for endogenous tagging using CRISPR technology in ToxoplasmaDepositorInsertLinker-6xHA-HGPRT-LoxP
UseExpression in toxoplasma gondiiTagsExpressionMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRARB-donor
Plasmid#132517PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertRARB (RARB Human)
UseCRISPRTagsGFPExpressionMutationnonePromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pINER2R
Plasmid#185882PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (11 copy).DepositorInsertPRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B>RPL25(partial; Arm2)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
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