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pET28C-mCherry-MED1LCD

Plasmid

#204404

Purpose

E. coli expression of MED1 low-complexity domain (947-1581) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

MED1(947-1581) (MED1 Human)

Use

Tags

6x-His; mCherry

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNAz-mBACEflag (C474A/C478A/C482A/C485A)

Plasmid

#26736

Depositor

Gopal Thinakaran

Article

Vetrivel et al J Biol Chem. 2009 Feb 6. 284(6):3793-803

Insert

BACE (Bace1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Cys 474, 478, 482, and 485 mutated to Ala

Promoter

Available since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
pCAG-OSF-KATNA1

Plasmid

#160051

Purpose

Mammalian expression vector for KATNA1 (KATANIN-P60) with N-terminal One-Strep-Flag tag. Internal ID: WISP20-18.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNA1 (KATNA1 Human)

Use

Tags

One-Strep-Flag (OSF)

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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Frt-V5-hspB6

Plasmid

#63107

Purpose

Expression of HSPB6 (small HSP) tagged with V5 in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb6 (HSPB6 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pBiFC-HA-hCaspase 2 FL (C303S)-VC155

Plasmid

#49257

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

caspase-2 (CASP2 Human)

Use

Tags

HA and split Venus C

Expression

Mammalian

Mutation

C303S

Promoter

CMV

Available since

March 14, 2014

Availability

Academic Institutions and Nonprofits only
His-GST-Cys-M87 Spastin

Plasmid

#184804

Purpose

Bacterial expression of M87 Spastin (A2C mutation)

Depositor

James Hurley

Article

Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

Insert

M87 Spastin (SPAST Human)

Use

Tags

His6-GST

Expression

Bacterial

Mutation

A2C

Promoter

T7

Available since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-mCherry-SPASTIN, F124D

Plasmid

#180648

Purpose

Lentiviral expression vector for SPASTIN. Has F124D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-44.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Starts at M87, F124D mutation, has siRNA resistan…

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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Frt-hspB6

Plasmid

#63097

Purpose

Expression of HSPB6 (small HSP) in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb6 (HSPB6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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mApple-M1 SpastinHFDD

Plasmid

#134462

Purpose

M1 SpastinHFDD expression

Depositor

Jennifer Lippincott-Schwartz

Article

Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

Insert

M1 Spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

Met to Ala at amino acid 87; His to Asp at amino …

Promoter

CMV

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pRK-FLAG-TRAF4(K190R)

Plasmid

#58317

Purpose

FLAG-tagged mouse TRAF4 K190R mutant

Depositor

Ying Zhang

Article

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

K 190 to R

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP-TRAF4(K190R)

Plasmid

#58319

Purpose

EGFP-tagged TRAF4 K190R mutant

Depositor

Ying Zhang

Article

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

K 190 to R

Promoter

CMV

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
pLVX-mCherry-SPASTIN, L177D

Plasmid

#180649

Purpose

Lentiviral expression vector for SPASTIN. Has L177D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-45.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Starts at M87, L177D mutation, has siRNA resistan…

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-THTAP-VPS9D1, residues 1-97

Plasmid

#180640

Purpose

Bacterial expression for VPS9D1 MIT domain, residues 1-97. Has N-terminal HIS-GST tag. Internal ID: WISP20-89.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

VPS9D1 (C16orf7 Human)

Use

Tags

HIS-GST

Expression

Bacterial

Mutation

Residues 1-97

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 KATNAL1 MIT residues 1-87

Plasmid

#180618

Purpose

Bacterial expression for KATNAL1 MIT domains, residues 1-87. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-54.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNAL1 (KATNAL1 Human)

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-87

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 KATNAL2 residues 1-98

Plasmid

#180619

Purpose

Bacterial expression for KATNAL2 , residues 1-98. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-28.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNAL2 (KATNAL2 Human)

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-98

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 RPS6KC1 MIT, residues 235-316

Plasmid

#180622

Purpose

Bacterial expression for RPS6KC1 MIT domain, residues 235-316. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-87.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

RPS6KC1 (RPS6KC1 Human)

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 235-316

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Myc-KATNB1

Plasmid

#160054

Purpose

Mammalian expression vector for KATNB1, (Katanin p80) with N-terminal Myc tag. Internal ID: WISP20-16.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNB1 (KATNB1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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gh109

Plasmid

#106768

Purpose

expression of gRNA targeting B3GALT6

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

B3GALT6 (B3GALT6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Zeo_BACE1

Plasmid

#227105

Purpose

BACE1 enzyme overexpression for Nrg1 activation in luciferase and barcode assays

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

BACE1 (BACE1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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Frt-V5-hspB8

Plasmid

#63109

Purpose

Expression of HSPB8 (small HSP) tagged with V5 in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb8 (HSPB8 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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