12,469 results

1. ELL3 Streptag CtoA

   Plasmid

   #127283

   Purpose

   Expresses mouse ELL3 with streptactin tag; To interrogate if the mutation decreases putative Myc activity

   Depositor

   Christine Milcarek

   Insert

   ELL3 (NM_145973) (ELL3 Mouse)
   

   Use

   Tags

   Twin strep-tag

   Expression

   Mammalian

   Mutation

   CEYLHNK to AEYLHQK

   Promoter

   EF1a

   Available since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MSCV-Lrrc40-trunc-IRES-BFP

   Plasmid

   #117268

   Purpose

   Retroviral overexpression of Lrrc40 - truncated version with seemingly same DE genes in Th2 as MSCV-Lrrc40-IRES-BFP

   Depositor

   Sarah Teichmann

   Article

   Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

   Insert

   Lrrc40 (Lrrc40 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   The insert is truncated - deletion of bp 334-407 …

   Promoter

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-hSyn-NOS-IN133.3xFLAG-WPRE

   Plasmid

   #127869

   Purpose

   pAAV plasmid expressing an NOS-IN133.3xFLAG fusion protein under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

   Insert

   Nos1 (Nos1 Mouse)
   

   Use

   AAV

   Tags

   3xFLAG

   Expression

   Mutation

   Amino acids 1-133 only

   Promoter

   hSyn

   Available since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA-FLAG-Intu

   Plasmid

   #128869

   Purpose

   For mammalian expression of FLAG-tagged mouse Inturned

   Depositor

   Aimin Liu

   Insert

   Inturned (Intu Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFB-U6-control-gRNA-hSyn-mCherry-WPRE-SV40pA

   Plasmid

   #128347

   Purpose

   pAAV encoding control gRNA for CRISPR gRNAs listed above

   Depositor

   Ryan Drenan

   Article

   Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

   Insert

   control (negative)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFB-U6-Chrnb4-gRNA-hSyn-mCherry-WPRE-SV40pA

   Plasmid

   #128345

   Purpose

   pAAV encoding gRNA sequence for loss-of-function indels

   Depositor

   Ryan Drenan

   Article

   Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

   Insert

   Chrnb4 (Chrnb4 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFB-U6-Chrna6-gRNA-hSyn-mCherry-WPRE-SV40pA

   Plasmid

   #128341

   Purpose

   pAAV encoding gRNA sequence for loss-of-function indels

   Depositor

   Ryan Drenan

   Article

   Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

   Insert

   Chrna6 (Chrna6 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ELL1 Streptag

   Plasmid

   #127278

   Purpose

   Expresses mouse ELL1 with streptactin tag in mammalian cells

   Depositor

   Christine Milcarek

   Insert

   ELL1 (NM_007924) (ELL Mouse)
   

   Use

   Tags

   Twin strep-tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Myc var1 K397R

   Plasmid

   #127285

   Purpose

   Expresses Myc with putative Ub site mutated

   Depositor

   Christine Milcarek

   Insert

   Myc (NM_001177352) (Myc Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K397R by Q5 mutagenesis

   Promoter

   CMV

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Myc var1

    Plasmid

    #127284

    Purpose

    Expresses Myc to see if degraded by ELLs

    Depositor

    Christine Milcarek

    Insert

    Myc (NM_001177352) (Myc Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    transcript variant 1

    Promoter

    CMV

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Myc var 1 K396/397 to NQ

    Plasmid

    #127286

    Purpose

    Expresses Myc with putative Ub site mutated

    Depositor

    Christine Milcarek

    Insert

    Myc (NM_001177352) (Myc Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K396 and 397 to N and Q by Q5 mutagenesi

    Promoter

    CMV

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. IgH intron enhancer

    Plasmid

    #127247

    Purpose

    To maximize gene expression in B cells

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    heavy chain enhancer
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FoxO6deltaCt

    Plasmid

    #124486

    Purpose

    Expresses dominant negative form of mouse FoxO6 with deleted C-term transactivation domain. T26A/S184A mutations increase nuclear localization of FoxO6.

    Depositor

    Anne Brunet

    Article

    Salih et al Genes Dev. 2012 Dec 15;26(24):2780-801.

    Insert

    FoxO6 (Foxo1, Foxo4, Foxo3, Foxo6 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T26A, S184A, transactivation domain deleted from …

    Promoter

    Available since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDNA3 mSmoothened NQ4

    Plasmid

    #126407

    Purpose

    Expression of Smoothened NQ4 in mammalian cell lines.

    Depositor

    Stacey Ogden

    Article

    Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

    Insert

    m-Smoothened (Smo Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Smo glycosylation site NQ4

    Promoter

    Available since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDNA3 mSmoothened NQ3

    Plasmid

    #126406

    Purpose

    Expression of Smoothened NQ3 in mammalian cell lines.

    Depositor

    Stacey Ogden

    Article

    Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

    Insert

    m-Smoothened (Smo Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Smo glycosylation site NQ3

    Promoter

    Available since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RV-mCD4-HH7-8

    Plasmid

    #124991

    Purpose

    Express the Helicobacter hepaticus specific TCRa/b HH7-8 in T cell hybridoma cells

    Depositor

    Dan Littman

    Article

    Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

    Insert

    HH7-8 TCRa P2A TCRb
    

    Use

    retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RV-mCD4-HH7-11

    Plasmid

    #124993

    Purpose

    Express the Helicobacter hepaticus specific TCRa/b HH7-11 in T cell hybridoma cells

    Depositor

    Dan Littman

    Article

    Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

    Insert

    HH7-11 TCRa P2A TCRb
    

    Use

    retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. RV-mCD4-HH7-9

    Plasmid

    #124992

    Purpose

    Express the Helicobacter hepaticus specific TCRa/b HH7-9 in T cell hybridoma cells

    Depositor

    Dan Littman

    Article

    Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

    Insert

    HH7-9 TCRa P2A TCRb
    

    Use

    retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RV-mCD4-HH7-6

    Plasmid

    #124990

    Purpose

    Express the Helicobacter hepaticus specific TCRa/b HH7-6 in T cell hybridoma cells

    Depositor

    Dan Littman

    Article

    Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

    Insert

    HH7-6 TCRa P2A TCRb
    

    Use

    retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RV-mCD4-HH5-4

    Plasmid

    #124984

    Purpose

    Express the Helicobacter hepaticus specific TCRa/b HH5-4 in T cell hybridoma cells

    Depositor

    Dan Littman

    Article

    Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

    Insert

    HH5-4 TCRa P2A TCRb
    

    Use

    retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. RV-mCD4-HH5-3

    Plasmid

    #124983

    Purpose

    Express the Helicobacter hepaticus specific TCRa/b HH5-3 in T cell hybridoma cells

    Depositor

    Dan Littman

    Article

    Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

    Insert

    TCR-HH5-3
    

    Use

    retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28B-PU.1-ETS-Wing

    Plasmid

    #124552

    Purpose

    Chimeric ETS domain: Wing of Ets-1 in PU.1 scaffold

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    The 5 residues making up the "wing" in …

    Promoter

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28B-PU.1-ETS-Loop

    Plasmid

    #124551

    Purpose

    Chimeric ETS domain: Loop of Ets-1 in PU.1 scaffold

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Residues 216 to 223 replaced with corresponding s…

    Promoter

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Anti-Synaptotagmin-12 [N277/7R]

    Plasmid

    #114482

    Purpose

    Mammalian Expression Plasmid of anti-Synaptotagmin-12 (Mouse). Derived from hybridoma N277/7.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Synaptotagmin-12 (Mus musculus) recombinant mouse monoclonal antibody (Syt12 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJET1.2_Bod1l_Anti-Sense

    Plasmid

    #124444

    Purpose

    Plasmid for anti-sense in situ probe in vitro transcription

    Depositor

    Yingguang Frank Chan

    Article

    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

    Insert

    Biorientation of chromosomes in cell division 1-like (Bod1l Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJET1.2_Nkx3-2_Anti-Sense

    Plasmid

    #124438

    Purpose

    Plasmid for anti-sense in situ probe in vitro transcription

    Depositor

    Yingguang Frank Chan

    Article

    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

    Insert

    NK3 homeobox 2 (Nkx3-2 Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. sgMlst8_1

    Plasmid

    #124909

    Purpose

    Targeting mouse Mlst8 gene by CRISPR-Cas9

    Depositor

    Jin Chen

    Article

    Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

    Insert

    Mlst8 (Mlst8 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. sgMlst8_2

    Plasmid

    #124910

    Purpose

    Targeting mouse Mlst8 gene by CRISPR-Cas9

    Depositor

    Jin Chen

    Article

    Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

    Insert

    Mlst8 (Mlst8 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Vmn2r1-5Hprt-loxP TV

    Plasmid

    #108079

    Purpose

    Tergeting vector for Vmn2r1 gene knockout mice (∆C1 strain)

    Depositor

    Peter Mombaerts

    Article

    Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

    Insert

    Vmn2r1-Hprt (5')-loxP-neo (Vmn2r1 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Vmn2r1-D-tauGFP TV

    Plasmid

    #108080

    Purpose

    Targeting vector for Vmn2r1 gene knockout mice (∆C1-GFP strain)

    Depositor

    Peter Mombaerts

    Article

    Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

    Insert

    Vmn2r1-D-tauGFP-pA-ACNF (Vmn2r1 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pISH-Vmn2r65

    Plasmid

    #108083

    Purpose

    Preparation of RNA probe for ISH of mouse Vmn2r65 gene

    Depositor

    Peter Mombaerts

    Article

    Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

    Insert

    Vmn2r65 (Vmn2r65 Mouse)
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pISH-Vmn2r76

    Plasmid

    #108084

    Purpose

    Preparation of RNA probe for ISH of mouse Vmn2r76 gene

    Depositor

    Peter Mombaerts

    Article

    Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

    Insert

    Vmn2r76 (Vmn2r76 Mouse)
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pISH-Vmn2r76-VR2

    Plasmid

    #108085

    Purpose

    Preparation of RNA probe for ISH of mouse Vmn2r76 gene

    Depositor

    Peter Mombaerts

    Article

    Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

    Insert

    Vmn2r76 (Vmn2r76 Mouse)
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pISH-Vmn2r118

    Plasmid

    #108086

    Purpose

    Preparation of RNA probe for ISH of mouse Vmn2r118 gene

    Depositor

    Peter Mombaerts

    Article

    Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

    Insert

    Vmn2r118 (Vmn2r118 Mouse)
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    changed T to C at nt 830 of NM_001104582.1

    Promoter

    Available since

    May 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pISH-Vmn2r120-TR

    Plasmid

    #108087

    Purpose

    Preparation of RNA probe for ISH of mouse Vmn2r120 gene

    Depositor

    Peter Mombaerts

    Article

    Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

    Insert

    Vmn2r120 (Vmn2r120 Mouse)
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    changed A to G at nt 1196 of NM_001104591.1

    Promoter

    Available since

    May 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA-mTRAF3ip3∆TM-CFP

    Plasmid

    #125182

    Purpose

    Expresses mouse TRAF3IP3 ∆TM in mammalian cells

    Depositor

    Shao-Cong Sun

    Insert

    TRAF3ip3 (Traf3ip3 Mouse)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    K87R and S359P- See depositor comments below

    Promoter

    CMV

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCLXSN(GFP)-HA-Tbkbp1 1-280

    Plasmid

    #125178

    Purpose

    Retroviral expression of mouse Tbkbp1 (1-280)

    Depositor

    Shao-Cong Sun

    Article

    Zhu et al Nat Commun. 2018 Jul 18;9(1):2812. doi:

    Insert

    Tbkbp1 (Tbkbp1 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-280

    Promoter

    CMV

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJET1.2_Nkx3-2_Sense

    Plasmid

    #124437

    Purpose

    Plasmid for sense in situ probe in vitro transcription

    Depositor

    Yingguang Frank Chan

    Article

    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

    Insert

    NK3 homeobox 2 (Nkx3-2 Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJET1.2_Gli3_Sense

    Plasmid

    #124439

    Purpose

    Plasmid for sense in situ probe in vitro transcription

    Depositor

    Yingguang Frank Chan

    Article

    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

    Insert

    GLI-Kruppel family member GLI3 (Gli3 Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJET1.2_Rab28_Sense

    Plasmid

    #124441

    Purpose

    Plasmid for sense in situ probe in vitro transcription

    Depositor

    Yingguang Frank Chan

    Article

    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

    Insert

    RAB28, member RAS oncogene family (Rab28 Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pJET1.2_Rab28_Anti-Sense

    Plasmid

    #124442

    Purpose

    Plasmid for anti-sense in situ probe in vitro transcription

    Depositor

    Yingguang Frank Chan

    Article

    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

    Insert

    RAB28, member RAS oncogene family (Rab28 Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pJET1.2_Bod1l_Sense

    Plasmid

    #124443

    Purpose

    Plasmid for sense in situ probe in vitro transcription

    Depositor

    Yingguang Frank Chan

    Article

    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

    Insert

    Biorientation of chromosomes in cell division 1-like (Bod1l Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCLXSN(GFP)-HA-Tbkbp1 1-330

    Plasmid

    #125179

    Purpose

    Retroviral expression of mouse Tbkbp1 (1-330)

    Depositor

    Shao-Cong Sun

    Article

    Zhu et al Nat Commun. 2018 Jul 18;9(1):2812. doi:

    Insert

    Tbkbp1 (Tbkbp1 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-330

    Promoter

    CMV

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET28B-PU.1-ETS-H3/S3

    Plasmid

    #124627

    Purpose

    Chimeric ETS domain: H3/S3 of Ets-1 in PU.1 scaffold

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Residues 237 to 241 replaced with corresponding s…

    Promoter

    Available since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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45. pET28B-PU.1-ETS-S3

    Plasmid

    #124550

    Purpose

    Chimeric ETS domain: S3 of Ets-1 in PU.1 scaffold

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Residues 242 to 246 replaced with corresponding s…

    Promoter

    Available since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pET28b-PU.1-ETS-H2

    Plasmid

    #123358

    Purpose

    Chimeric ETS domain: H2 of Ets-1 in PU.1 scaffold

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Residues 207 to 215 replaced with corresponding s…

    Promoter

    Available since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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47. pKLV2-U6(BsmBI)-EF1a-BFP-BSD-Nkx2-5

    Plasmid

    #117137

    Purpose

    Expression vector encoding BFP-BSD-Nkx2-5

    Depositor

    Kosuke Yusa

    Article

    Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste

    Insert

    Nkx2-5 CDS (Nkx2-5 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    modified BFP

    Promoter

    human U6 promoter, human EF1a promoter

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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48. KA1270_pPB-gPou5f1_3.5kb-UBCmin-IP

    Plasmid

    #124181

    Purpose

    piggyBac-based reporter (Puro) driven by the Pou5f1 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    IRES-Pac, Pou5f1 enhancer
    

    Use

    Reporter

    Tags

    Expression

    Mutation

    Promoter

    UBCmin

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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49. pZDonor-U6-myogenin stop codon gRNA

    Plasmid

    #69549

    Purpose

    Expresses guide RNA targeting near the stop codon of myogenin in the mouse genome.

    Depositor

    Charles Gersbach

    Insert

    Myogenin C terminus-targeting guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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50. pMX-eSOX2AHK

    Plasmid

    #118826

    Purpose

    Retroviral expression of a mutant of Sox2 that improves efficiency of iPSC generation

    Depositor

    Ralf Jauch

    Article

    Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

    Insert

    eSox2AHK (Sox2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    AHK: E46A, I53H

    Promoter

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge